Relation Extraction Examples

(0)

CheY - dependent methylation of the asparagine receptor, McpB, during chemotaxis in Bacillus subtilis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9977	Methylation	CheY - dependent Methylationmethylation of the asparagine receptor, ProteinMcpB , during chemotaxis in Bacillus subtilis.

(1)

For the Gram - positive organism Bacillus subtilis, chemotaxis to the attractant asparagine is mediated by the chemoreceptor McpB.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(2)

In this study, we show that rapid net demethylation of B. subtilis McpB results in the immediate production of methanol, presumably due to the action of CheB.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Relation	Text (Head - Tail)
0.9996	Methylation	In this study, we show that rapid net Methylationdemethylation of B. subtilis ProteinMcpB results in the immediate production of methanol, presumably due to the action of CheB.
	Methylation	In this study, we show that rapid net Demethylationdemethylation of B. subtilis ProteinMcpB results in the immediate production of methanol, presumably due to the action of CheB.

(3)

We also show that net demethylation of McpB occurs upon both addition and removal of asparagine.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	We also show that net Methylationdemethylation of ProteinMcpB occurs upon both addition and removal of asparagine.
	Methylation	We also show that net Demethylationdemethylation of ProteinMcpB occurs upon both addition and removal of asparagine.

(4)

After each demethylation event, McpB is remethylated to nearly prestimulus levels.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	After each demethylation event, ProteinMcpB is Methylationremethylated to nearly prestimulus levels.

(5)

Both remethylation events are attributable to CheR using S - adenosylmethionine as a substrate.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	Both Methylationremethylation events are attributable to ProteinCheR using S - adenosylmethionine as a substrate.

(6)

Therefore, no methyl transfer to an intermediate carrier need be postulated to occur during chemotaxis in B. subtilis as was previously suggested.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(7)

Furthermore, we show that the remethylation of asparagine - bound McpB requires the response regulator, CheY - P, suggesting that CheY - P acts in a feedback mechanism to facilitate adaptation to positive stimuli during chemotaxis in B. subtilis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9997	Methylation	Furthermore, we show that the Methylationremethylation of asparagine - bound ProteinMcpB requires the response regulator, CheY - P, suggesting that CheY - P acts in a feedback mechanism to facilitate adaptation to positive stimuli during chemotaxis in B. subtilis.

(8)

This hypothesis is supported by two observations : a cheRBCD mutant is capable of transient excitation and subsequent oscillations that bring the flagellar rotational bias below the prestimulus value in the tethered cell assay, and the cheRBCD mutant is capable of swarming in a Tryptone swarm plate.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(9)

Transglycosylation reactions of Bacillus stearothermophilus maltogenic amylase with acarbose and various acceptors.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(10)

It was observed that Bacillus stearothermophilus maltogenic amylase cleaved the first glycosidic bond of acarbose to produce glucose and a pseudotrisaccharide ( PTS ) that was transferred to C - 6 of the glucose to give an alpha - ( 1 - - > 6 ) glycosidic linkage and the formation of isoacarbose.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(11)

The addition of a number of different carbohydrates to the digest gave transfer products in which PTS was primarily attached alpha - ( 1 - - > 6 ) to D - glucose, D - mannose, D - galactose, and methyl alpha - D - glucopyranoside.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(12)

With D - fructopyranose and D - xylopyranose, PTS was linked alpha - ( 1 - - > 5 ) and alpha - ( 1 - - > 4 ), respectively.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(13)

PTS was primarily transferred to C - 6 of the nonreducing residue of maltose, cellobiose, lactose, and gentiobiose.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(14)

Lesser amounts of alpha - ( 1 - - > 3 ) and / or alpha - ( 1 - - > 4 ) transfer products were also observed for these carbohydrate acceptors.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(15)

The major transfer product to sucrose gave PTS linked alpha - ( 1 - - > 4 ) to the glucose residue. alpha, alpha - Trehalose gave two major products with PTS linked alpha - ( 1 - - > 6 ) and alpha - ( 1 - - > 4 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(16)

Maltitol gave two major products with PTS linked alpha - ( 1 - - > 6 ) and alpha - ( 1 - - > 4 ) to the glucopyranose residue.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(17)

Raffinose gave two major products with PTS linked alpha - ( 1 - - > 6 ) and alpha - ( 1 - - > 4 ) to the D - galactopyranose residue.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(18)

Maltotriose gave two major products with PTS linked alpha - ( 1 - - > 6 ) and alpha - ( 1 - - > 4 ) to the nonreducing end glucopyranose residue.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(19)

Xylitol gave PTS linked alpha - ( 1 - - > 5 ) as the major product and D - glucitol gave PTS linked alpha - ( 1 - - > 6 ) as the only product.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(20)

The structures of the transfer products were determined using thin - layer chromatography, high - performance ion chromatography, enzyme hydrolysis, methylation analysis and 13C NMR spectroscopy.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(21)

The best acceptor was gentiobiose, followed closely by maltose and cellobiose, and the weakest acceptor was D - glucitol.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(22)

A Plasmodium chabaudi chabaudi high molecular mass glycoprotein translocated to the host cell membrane by a non - classical secretory pathway.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(23)

We have purified and characterized a novel high molecular mass glycoprotein of P. chabaudi chabaudi ( Pc550gp ) that is transported to the erythrocyte membrane during the intraerythrocytic cycle.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(24)

Immuno fluorescence assays with polyclonal monospecific antibodies against Pc550gp show that the protein to be localized in the periphery of young trophozoite stages i. e., on the plasma membrane or parasitophorous vacuole membrane.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(25)

However, in late trophozoites and schizonts the antigen is distributed in both parasite and host cell membranes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(26)

These results were confirmed by immunoblotting of isolated parasites and infected host cell membranes at different stages of parasite development.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(27)

Moreover, alkali extraction of purified infected erythrocyte membranes at mature stages of parasite development does not solubilize Pc550gp, suggesting that it is an integral membrane protein.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(28)

In addition proteinase K digestion of intact infected host cells induced the disappearance of Pc550gp.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(29)

Further indicating its transmembrane nature and that it presents extracellular domains susceptible to proteolysis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(30)

Brefeldin A or low temperature ( 15 degrees C ) treatment did not affect the translocation of Pc550gp from the parasite compartments to the erythrocyte membrane, indicating that the secretion of Pc550gp does not follow the classical transport pathway described in most eukaryotic cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(31)

Novel roles for classical factors at the interface between translation termination and initiation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(32)

The pathway of bacterial ribosome recycling following translation termination has remained obscure.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(33)

Here, we elucidate two essential steps and describe the roles played by the three translation factors EF - G, RRF, and IF3.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(34)

Release factor RF3 is known to catalyze the dissociation of RF1 or RF2 from ribosomes after polypeptide release.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(35)

We show that the next step is dissociation of 50S subunits from the 70S posttermination complex and that it is catalyzed by RRF and EF - G and requires GTP hydrolysis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(36)

Removal of deacylated tRNA from the resulting 30S : mRNA : tRNA posttermination complex is then necessary to permit rapid 30S subunit recycling.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(37)

We show that this step requires initiation factor IF3, whose role was previously thought to be restricted to promoting specific 30S initiation complex formation from free 30S subunits.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(38)

Effect of alternative glycosylation on insulin receptor processing.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	Effect of alternative Glycosylationglycosylation on Proteininsulin receptor processing.

(39)

The mature insulin receptor is a cell surface heterotetrameric glycoprotein composed of two alpha - and two beta - subunits.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(40)

In 3T3 - L1 adipocytes as in other cell types, the receptor is synthesized as a single polypeptide consisting of uncleaved alpha - and beta - subunits, migrating as a 190 - kDa glycoprotein.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(41)

To examine the importance of N - linked glycosylation on insulin receptor processing, we have used glucose deprivation as a tool to alter protein glycosylation.

Precision: 11.11

Recall: 100.00

F1: 20.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	To examine the importance of GlycosylationN - linked glycosylation on Proteininsulin receptor processing, we have used glucose deprivation as a tool to alter protein glycosylation.
1.0000	Methylation	To examine the importance of N - linked Glycosylationglycosylation on Proteininsulin receptor processing, we have used glucose deprivation as a tool to alter protein glycosylation.
0.9868	Methylation	To examine the importance of GlycosylationN - linked glycosylation on Proteininsulin receptor processing, we have used glucose deprivation as a tool to alter protein glycosylation.
0.8955	Methylation	To examine the importance of GlycosylationN - linked glycosylation Glycosylationglycosylation on insulin receptor processing, we have used glucose deprivation as a tool to alter protein glycosylation.
0.8526	Methylation	To examine the importance of GlycosylationN - linked glycosylation Glycosylationglycosylation on insulin receptor processing, we have used glucose deprivation as a tool to alter protein glycosylation.
0.7830	Methylation	To examine the importance of GlycosylationN - linked GlycosylationN - linked glycosylation on insulin receptor processing, we have used glucose deprivation as a tool to alter protein glycosylation.
0.7803	Methylation	To examine the importance of GlycosylationN - linked Glycosylationglycosylation on insulin receptor processing, we have used glucose deprivation as a tool to alter protein glycosylation.
0.7278	Methylation	To examine the importance of GlycosylationN - linked Glycosylationglycosylation on insulin receptor processing, we have used glucose deprivation as a tool to alter protein glycosylation.
0.6470	Methylation	To examine the importance of GlycosylationN - linked glycosylation GlycosylationN - linked glycosylation on insulin receptor processing, we have used glucose deprivation as a tool to alter protein glycosylation.

(42)

Western blot analysis shows that glucose deprivation led to a time - dependent accumulation of an alternative proreceptor of 170 kDa in a subcellular fraction consistent with endoplasmic reticulum localization.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(43)

Co - precipitation assays provide evidence that the alternative proreceptor bound GRP78, an endoplasmic reticulum molecular chaperone.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(44)

N - Glycosidase F treatment shows that the alternative proreceptor contained N - linked oligosaccharides.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9957	Methylation	N - Glycosidase F treatment shows that the alternative proreceptor contained GlycosylationN - linked Entityoligosaccharides .

(45)

Yet, endoglycosidase H insensitivity indicates an aberrant oligosaccharide structure.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(46)

Using pulse - chase methodology, we show that the synthetic rate was similar between the normal and alternative proreceptor.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(47)

However, the normal proreceptor was processed into alpha - and beta - subunits ( t ( ( 1 ) / ( 2 ) ) = 1. 3 + / - 0. 6 h ), while the alternative proreceptor was degraded ( t ( ( 1 ) / ( 2 ) ) = 5. 1 + / - 0. 6 h ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(48)

Upon refeeding cells that were initially deprived of glucose, the alternative proreceptor was processed to a higher molecular weight form and gained sensitivity to endoglycosidase H.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(49)

This " intermediate " form of the proreceptor was also degraded, although a small fraction escaped degradation, resulting in cleavage to the alpha - and beta - subunits.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(50)

These data provide evidence for the first time that glucose deprivation leads to the accumulation of an alternative proreceptor, which can be post - translationally glycosylated with the readdition of glucose inducing both accelerated degradation and maturation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(51)

Meat, metabolic genotypes and risk for colorectal cancer.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(52)

Persuasive data exist as to the importance of environmental factors in the pathogenesis of sporadic colorectal cancer.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(53)

One possibility is that the effect of environmental factors varies between individuals, perhaps on the basis of inherited variation ( polymorphism ) in genes which influence the activation or inactivation of dietary carcinogens.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(54)

Thus far, the focus has been on acetylator genes ( NAT1, NAT2 ) and the activation of heterocyclic amines, carcinogens generated by cooking meat for prolonged periods at high temperature.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(55)

Three case - control studies and one prospective study have shown a consistent trend towards higher risks for cancer with higher intakes of meat in rapid acetylators for NAT1, NAT2 or both genotypes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(56)

Other links between meat, cooking methods, metabolic genotypes and risk for cancer might include enhanced activation of polycyclic aromatic hydrocarbons and N - nitroso compounds by variant genotypes of CYP1A1 and CYP2E1, respectively, and modulation by meat of the protective effect of the E4 allele of apolipoprotein E on risk for cancer of the proximal colon.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(57)

Differential expression of human lysyl hydroxylase genes, lysine hydroxylation, and cross - linking of type I collagen during osteoblastic differentiation in vitro.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(58)

The pattern of lysyl hydroxylation in the nontriple helical domains of collagen is critical in determining the cross - linking pathways that are tissue specific.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(59)

We hypothesized that the tissue specificity of type I collagen cross - linking is, in part, due to the differential expression of lysyl hydroxylase genes ( Procollagen - lysine, 2 - oxyglutarate, 5 - dioxygenase 1, 2, and 3 [ PLOD1, PLOD2, and PLOD3 ] ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(60)

In this study, we have examined the expression patterns of these three genes during the course of in vitro differentiation of human osteoprogenitor cells ( bone marrow stromal cells [ BMSCs ] ) and normal skin fibroblasts ( NSFs ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(61)

In addition, using the medium and cell layer / matrix fractions in these cultures, lysine hydroxylation of type I collagen alpha chains and collagen cross - linking chemistries have been characterized.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(62)

High levels of PLOD1 and PLOD3 genes were expressed in both BMSCs and NSFs, and the expression levels did not change in the course of differentiation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(63)

In contrast to the PLOD1 and PLOD3 genes, both cell types showed low PLOD2 gene expression in undifferentiated and early differentiated conditions.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(64)

However, fully differentiated BMSCs, but not NSFs, exhibited a significantly elevated level ( 6 - fold increase ) of PLOD2 mRNA.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(65)

This increase coincided with the onset of matrix mineralization and with the increase in lysyl hydroxylation in the nontriple helical domains of alpha chains of type I collagen molecule.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(66)

Furthermore, the collagen cross - links that are derived from the nontriple helical hydroxylysine - aldehyde were found only in fully differentiated BMSC cultures.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(67)

The data suggests that PLOD2 expression is associated with lysine hydroxylation in the nontriple helical domains of collagen and, thus, could be partially responsible for the tissue - specific collagen cross - linking pattern.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(68)

Phenylalanine residues in the active site of tyrosine hydroxylase : mutagenesis of Phe300 and Phe309 to alanine and metal ion - catalyzed hydroxylation of Phe300.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(69)

Residues Phe300 and Phe309 of tyrosine hydroxylase are located in the active site in the recently described three - dimensional structure of the enzyme, where they have been proposed to play roles in substrate binding.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(70)

Also based on the structure, Phe300 has been reported to be hydroxylated due to a naturally occurring posttranslational modification [ Goodwill, K.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(71)

E., Sabatier, C., and Stevens, R.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(72)

C. ( 1998 ) Biochemistry 37, 13437 - 13445 ].

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(73)

Mutants of tyrosine hydroxylase with alanine substituted for Phe300 or Phe309 have now been purified and characterized.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(74)

The F309A protein possesses 40 % less activity than wild - type tyrosine hydroxylase in the production of DOPA, but full activity in the production of dihydropterin.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(75)

The F300A protein shows a 2. 5 - fold decrease in activity in the production of both DOPA and dihydropterin.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(76)

The K ( 6 - MPH4 ) value for F300A tyrosine hydroxylase is twice the wild - type value.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(77)

These results are consistent with Phe309 having a role in maintaining the integrity of the active site, while Phe300 contributes less than 1 kcal / mol to binding tetrahydropterin.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(78)

Characterization of Phe300 by MALDI - TOF mass spectrometry and amino acid sequencing showed that hydroxylation only occurs in the isolated catalytic domain after incubation with a large excess of 7, 8 - dihydropterin, DTT, and Fe ( 2 + ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(79)

The modification is not observed in the untreated catalytic domain or in the full - length protein, even in the presence of excess iron.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(80)

These results establish that hydroxylation of Phe300 is an artifact of the crystallography conditions and is not relevant to catalysis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(81)

Development of an enzyme - linked immunosorbent assay, using a monoclonal antibody against alpha2 - macroglobulin, for the diagnosis of systemic lupus erythematosus.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(82)

OBJECTIVES : To develop an enzyme - linked immunosorbent assay ( ELISA ) using a monoclonal antibody ( mab ) directed against abnormally glycosylated serum alpha2 - macroglobulin ( alpha2 - M ) from patients with systemic lupus erythematosus ( SLE ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	OBJECTIVES : To develop an enzyme - linked immunosorbent assay ( ELISA ) using a monoclonal antibody ( mab ) directed against abnormally Glycosylationglycosylated serum Proteinalpha2 - macroglobulin ( alpha2 - M ) from patients with systemic lupus erythematosus ( SLE ).

(83)

DESIGN AND METHODS : Serum alpha2 - M purified by HPLC from patients with SLE was injected in a Balb / c, CB6 F1 female mouse and hybrid cell lines were screened using alpha2 - M Glu - C fragments derived from SLE and normal donors ( NHS ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(84)

A mab was selected and used to develop an ELISA by which sera from NHS ( n = 14 ), SLE ( n = 34 ), rheumatoid arthritis ( n = 15 ), Sjogren's syndrome ( n = 11 ), mixed connective tissue diseases ( n = 12 ), and liver diseases ( n = 11 ) were analyzed.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(85)

RESULTS : The affinity of the mab for alpha2 - M from SLE, but not from the other diseases, was higher compared to NHS, as demonstrated by immunoblotting and ELISA.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(86)

CONCLUSIONS : The ELISA was capable of recognizing changes of glycosylation of alpha2 - M in SLE and may be useful for its differential diagnosis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	CONCLUSIONS : The ELISA was capable of recognizing changes of Glycosylationglycosylation of Proteinalpha2 - M in SLE and may be useful for its differential diagnosis.

(87)

Efficient adaptational demethylation of chemoreceptors requires the same enzyme - docking site as efficient methylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(88)

The mechanistic basis of sensory adaptation and gradient sensing in bacterial chemotaxis is reversible covalent modification of transmembrane chemoreceptors, methylation, and demethylation at specific glutamyl residues in their cytoplasmic domains.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(89)

These reactions are catalyzed by a dedicated methyltransferase CheR and a dedicated methylesterase CheB.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(90)

The esterase is also a deamidase that creates certain methyl - accepting glutamyls by hydrolysis of glutamine side chains.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(91)

We investigated the action of CheB and its activated form, phospho - CheB, on a truncated form of the aspartate receptor of Escherichia coli that was missing the last 5 aa of the intact receptor.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(92)

The deleted pentapeptide is conserved in several chemoreceptors in enteric and related bacteria.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(93)

The truncated receptor was much less efficiently demethylated and deamidated than intact receptor, but essentially was unperturbed for kinase activation or transmembrane signaling.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(94)

CheB bound specifically to an affinity column carrying the isolated pentapeptide, implying that in the intact receptor the pentapeptide serves as a docking site for the methylesterase / deamidase and that the truncated receptor was inefficiently modified because the enzyme could not dock.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(95)

It is striking that the same pentapeptide serves as an activity - enhancing docking site for the methyltransferase CheR, the other enzyme involved in adaptational covalent modification of chemoreceptors.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(96)

A shared docking site raises the tantalizing possibility that relative rates of methylation and demethylation could be influenced by competition between the two enzymes at that site.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(97)

Roles of cell division and gene transcription in the methylation of CpG islands.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(98)

De novo methylation of CpG islands within the promoters of eukaryotic genes is often associated with their transcriptional repression, yet the methylation of CpG islands located downstream of promoters does not block transcription.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(99)

We investigated the kinetics of mRNA induction, demethylation, and remethylation of the p16 promoter and second - exon CpG islands in T24 cells after 5 - aza - 2'- deoxycytidine ( 5 - Aza - CdR ) treatment to explore the relationship between CpG island methylation and gene transcription.

Precision: 42.86

Recall: 60.00

F1: 50.00

Score	Relation	Text (Head - Tail)
0.9980	Methylation	We investigated the kinetics of mRNA induction, demethylation, and DNA_methylationremethylation of the p16 Entitypromoter and second - exon CpG islands in T24 cells after 5 - aza - 2'- deoxycytidine ( 5 - Aza - CdR ) treatment to explore the relationship between CpG island methylation and gene transcription.
0.9950	Methylation	We investigated the kinetics of mRNA induction, demethylation, and DNA_methylationremethylation of the p16 promoter and Entitysecond - exon CpG islands in T24 cells after 5 - aza - 2'- deoxycytidine ( 5 - Aza - CdR ) treatment to explore the relationship between CpG island methylation and gene transcription.
0.9788	Methylation	We investigated the kinetics of mRNA induction, demethylation, and remethylation of the p16 promoter and second - exon CpG islands in T24 cells after 5 - aza - 2'- deoxycytidine ( 5 - Aza - CdR ) treatment to explore the relationship between EntityCpG island DNA_methylationmethylation and gene transcription.
0.9943	Methylation	We investigated the kinetics of mRNA induction, DNA_methylationdemethylation , and remethylation of the p16 Entitypromoter and second - exon CpG islands in T24 cells after 5 - aza - 2'- deoxycytidine ( 5 - Aza - CdR ) treatment to explore the relationship between CpG island methylation and gene transcription.
0.9931	Methylation	We investigated the kinetics of mRNA induction, DNA_methylationdemethylation , and remethylation of the p16 promoter and Entitysecond - exon CpG islands in T24 cells after 5 - aza - 2'- deoxycytidine ( 5 - Aza - CdR ) treatment to explore the relationship between CpG island methylation and gene transcription.
0.9921	Methylation	We investigated the kinetics of mRNA induction, demethylation, and DNA_methylationremethylation of the p16 promoter and second - exon EntityCpG islands in T24 cells after 5 - aza - 2'- deoxycytidine ( 5 - Aza - CdR ) treatment to explore the relationship between CpG island methylation and gene transcription.
0.9882	Methylation	We investigated the kinetics of mRNA induction, DNA_methylationdemethylation , and remethylation of the p16 promoter and second - exon EntityCpG islands in T24 cells after 5 - aza - 2'- deoxycytidine ( 5 - Aza - CdR ) treatment to explore the relationship between CpG island methylation and gene transcription.
	Methylation	We investigated the kinetics of mRNA induction, DNA_demethylationdemethylation , and remethylation of the p16 promoter and Entitysecond - exon CpG islands in T24 cells after 5 - aza - 2'- deoxycytidine ( 5 - Aza - CdR ) treatment to explore the relationship between CpG island methylation and gene transcription.
	Methylation	We investigated the kinetics of mRNA induction, DNA_demethylationdemethylation , and remethylation of the p16 Entitypromoter and second - exon CpG islands in T24 cells after 5 - aza - 2'- deoxycytidine ( 5 - Aza - CdR ) treatment to explore the relationship between CpG island methylation and gene transcription.

(100)

The rates of remethylation of both CpG islands were associated with time but not with the rate of cell division, and remethylation of the p16 exon 2 CpG island occurred at a higher rate than that of the p16 promoter.

Precision: 40.00

Recall: 100.00

F1: 57.14

Score	Relation	Text (Head - Tail)
0.9988	Methylation	The rates of remethylation of both CpG islands were associated with time but not with the rate of cell division, and DNA_methylationremethylation of the p16 Entityexon 2 CpG island occurred at a higher rate than that of the p16 promoter.
0.9892	Methylation	The rates of remethylation of both CpG islands were associated with time but not with the rate of cell division, and DNA_methylationremethylation of the p16 exon 2 CpG island occurred at a higher rate than that of the p16 Entitypromoter .
0.9999	Methylation	The rates of DNA_methylationremethylation of both EntityCpG islands were associated with time but not with the rate of cell division, and remethylation of the p16 exon 2 CpG island occurred at a higher rate than that of the p16 promoter.
0.9935	Methylation	The rates of remethylation of both CpG islands were associated with time but not with the rate of cell division, and DNA_methylationremethylation of the p16 exon 2 EntityCpG island occurred at a higher rate than that of the p16 promoter.
0.4095	Methylation	The rates of DNA_methylationremethylation of both CpG islands were associated with time but not with the rate of cell division, and remethylation of the p16 Entityexon 2 CpG island occurred at a higher rate than that of the p16 promoter.

(101)

We also examined the relationship between the remethylation of coding sequence CpG islands and gene transcription.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(102)

The kinetics of remethylation of the p16 exon 2, PAX - 6 exon 5, c - ABL exon 11, and MYF - 3 exon 3 loci were examined following 5 - Aza - CdR treatment because these genes contain exonic CpG islands which are hypermethylated in T24 cells.

Precision: 66.67

Recall: 100.00

F1: 80.00

Score	Relation	Text (Head - Tail)
0.9991	Methylation	The kinetics of DNA_methylationremethylation of the p16 Entityexon 2 , PAX - 6 exon 5, c - ABL exon 11, and MYF - 3 exon 3 loci were examined following 5 - Aza - CdR treatment because these genes contain exonic CpG islands which are hypermethylated in T24 cells.
0.9985	Methylation	The kinetics of remethylation of the p16 exon 2, PAX - 6 exon 5, c - ABL exon 11, and MYF - 3 Entityexon 3 loci were examined following 5 - Aza - CdR treatment because these genes contain exonic CpG islands which are DNA_methylationhypermethylated in T24 cells.
0.9966	Methylation	The kinetics of remethylation of the p16 exon 2, PAX - 6 Entityexon 5 , c - ABL exon 11, and MYF - 3 exon 3 loci were examined following 5 - Aza - CdR treatment because these genes contain exonic CpG islands which are DNA_methylationhypermethylated in T24 cells.
0.9964	Methylation	The kinetics of remethylation of the p16 exon 2, PAX - 6 exon 5, c - ABL Entityexon 11 , and MYF - 3 exon 3 loci were examined following 5 - Aza - CdR treatment because these genes contain exonic CpG islands which are DNA_methylationhypermethylated in T24 cells.
0.9959	Methylation	The kinetics of DNA_methylationremethylation of the p16 exon 2, PAX - 6 Entityexon 5 , c - ABL exon 11, and MYF - 3 exon 3 loci were examined following 5 - Aza - CdR treatment because these genes contain exonic CpG islands which are hypermethylated in T24 cells.
0.9953	Methylation	The kinetics of DNA_methylationremethylation of the p16 exon 2, PAX - 6 exon 5, c - ABL exon 11, and MYF - 3 Entityexon 3 loci were examined following 5 - Aza - CdR treatment because these genes contain exonic CpG islands which are hypermethylated in T24 cells.
0.9943	Methylation	The kinetics of DNA_methylationremethylation of the p16 exon 2, PAX - 6 exon 5, c - ABL Entityexon 11 , and MYF - 3 exon 3 loci were examined following 5 - Aza - CdR treatment because these genes contain exonic CpG islands which are hypermethylated in T24 cells.
0.9932	Methylation	The kinetics of remethylation of the p16 Entityexon 2 , PAX - 6 exon 5, c - ABL exon 11, and MYF - 3 exon 3 loci were examined following 5 - Aza - CdR treatment because these genes contain exonic CpG islands which are DNA_methylationhypermethylated in T24 cells.
0.9966	Methylation	The kinetics of remethylation of the p16 exon 2, PAX - 6 exon 5, c - ABL exon 11, and MYF - 3 Entityexon 3 loci were examined following 5 - Aza - CdR treatment because these genes contain exonic CpG islands which are DNA_methylationhypermethylated in T24 cells.
0.9911	Methylation	The kinetics of DNA_methylationremethylation of the p16 exon 2, PAX - 6 exon 5, c - ABL exon 11, and MYF - 3 Entityexon 3 loci were examined following 5 - Aza - CdR treatment because these genes contain exonic CpG islands which are hypermethylated in T24 cells.
0.9892	Methylation	The kinetics of remethylation of the p16 exon 2, PAX - 6 Entityexon 5, c - ABL exon 11, and MYF - 3 exon 3 loci were examined following 5 - Aza - CdR treatment because these genes contain exonic CpG islands which are DNA_methylationhypermethylated in T24 cells.
0.9873	Methylation	The kinetics of DNA_methylationremethylation of the p16 exon 2, PAX - 6 Entityexon 5, c - ABL exon 11, and MYF - 3 exon 3 loci were examined following 5 - Aza - CdR treatment because these genes contain exonic CpG islands which are hypermethylated in T24 cells.

(103)

Remethylation occurred most rapidly in the p16, PAX - 6, and c - ABL genes, shown to be transcribed prior to drug treatment.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9997	Methylation	DNA_methylationRemethylation occurred most rapidly in the p16, ProteinPAX - 6 , and c - ABL genes, shown to be transcribed prior to drug treatment.
0.9996	Methylation	DNA_methylationRemethylation occurred most rapidly in the Proteinp16 , PAX - 6, and c - ABL genes, shown to be transcribed prior to drug treatment.
0.9994	Methylation	DNA_methylationRemethylation occurred most rapidly in the p16, PAX - 6, and Proteinc - ABL genes, shown to be transcribed prior to drug treatment.

(104)

These regions also exhibited higher levels of remethylation in single - cell clones and subclones derived from 5 - Aza - CdR - treated T24 cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(105)

Our data suggest that de novo methylation is not restricted to the S phase of the cell cycle and that transcription through CpG islands does not inhibit their remethylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(106)

Distinct methylation pattern and microsatellite instability in sporadic gastric cancer.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(107)

Aberrant 5'CpG island methylation is an alternative mechanism of gene inactivation during the development of cancer as demonstrated for several tumor - suppressor genes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(108)

Also, marked relationship of microsatellite instability ( MSI ) and DNA methylation has been reported in sporadic colorectal cancer, which is a result of epigenetic inactivation of hMLH1 in association of promoter hypermethylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9997	Methylation	Also, marked relationship of microsatellite instability ( MSI ) and DNA methylation has been reported in sporadic colorectal cancer, which is a result of epigenetic inactivation of hMLH1 in association of Entitypromoter DNA_methylationhypermethylation .

(109)

In the present study, we investigated the 5'CpG island hypermethylation of hMLH1, E - cadherin and p16 in 61 primary gastric cancers ( GCs ) by using combined bisulfite restriction analysis ( COBRA ) and methylation - specific PCR ( MSP ), and their MSI status.

Precision: 25.00

Recall: 100.00

F1: 40.00

Score	Relation	Text (Head - Tail)
0.9990	Methylation	In the present study, we investigated the Entity5'CpG island DNA_methylationhypermethylation of hMLH1, E - cadherin and p16 in 61 primary gastric cancers ( GCs ) by using combined bisulfite restriction analysis ( COBRA ) and methylation - specific PCR ( MSP ), and their MSI status.
0.9993	Methylation	In the present study, we investigated the 5 ' EntityCpG island DNA_methylationhypermethylation of hMLH1, E - cadherin and p16 in 61 primary gastric cancers ( GCs ) by using combined bisulfite restriction analysis ( COBRA ) and methylation - specific PCR ( MSP ), and their MSI status.
0.8317	Methylation	In the present study, we investigated the 5 ' EntityCpG island DNA_methylationhypermethylation of hMLH1, E - cadherin and p16 in 61 primary gastric cancers ( GCs ) by using combined bisulfite restriction analysis ( COBRA ) and methylation - specific PCR ( MSP ), and their MSI status.
0.7899	Methylation	In the present study, we investigated the Entity5'CpG island DNA_methylationhypermethylation of hMLH1, E - cadherin and p16 in 61 primary gastric cancers ( GCs ) by using combined bisulfite restriction analysis ( COBRA ) and methylation - specific PCR ( MSP ), and their MSI status.

(110)

Of 61 GCs investigated, 5 ( 8. 1 % ) tumors presented hMLH1 methylation, 16 ( 26. 2 % ) and 25 ( 40. 9 % ) showed E - cadherin and p16 methylation respectively, and 8 ( 13. 1 % ) presented high - frequency MSI ( MSI - H ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(111)

Of the 8 MSI - H patients, 5 presented hMLH1 methylation, whereas no low - frequency MSI ( MSI - L ) and microsatellite stable ( MSS ) cases exhibited hMLH1 methylation ( 5 / 8 vs. 0 / 43, p < 0. 00001 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9982	Methylation	Of the 8 MSI - H patients, 5 presented hMLH1 methylation, whereas no low - frequency MSI ( MSI - L ) and microsatellite stable ( MSS ) cases exhibited ProteinhMLH1 DNA_methylationmethylation ( 5 / 8 vs. 0 / 43, p < 0. 00001 ).
0.9978	Methylation	Of the 8 MSI - H patients, 5 presented ProteinhMLH1 DNA_methylationmethylation , whereas no low - frequency MSI ( MSI - L ) and microsatellite stable ( MSS ) cases exhibited hMLH1 methylation ( 5 / 8 vs. 0 / 43, p < 0. 00001 ).

(112)

Furthermore, these patients also presented E - cadherin and p16 hypermethylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9997	Methylation	Furthermore, these patients also presented ProteinE - cadherin and p16 DNA_methylationhypermethylation .
0.9979	Methylation	Furthermore, these patients also presented E - cadherin and Proteinp16 DNA_methylationhypermethylation .

(113)

Our data showed a significant correlation between hMLH1 methylation and MSI in GC, and suggested that a common mechanism of aberrant de novo methylation can be postulated in these cancers.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9984	Methylation	Our data showed a significant correlation between ProteinhMLH1 DNA_methylationmethylation and MSI in GC, and suggested that a common mechanism of aberrant de novo methylation can be postulated in these cancers.

(114)

Effect of HSP47 on prolyl 4 - hydroxylation of collagen model peptides.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(115)

Prolyl 4 - hydroxylation, the most important post - translational modification in collagen biosynthesis, is catalyzed by prolyl 4 - hydroxylase, an endoplasmic reticulum - resident enzyme.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(116)

HSP47 is a collagen - binding stress protein which also resides in the endoplasmic reticulum ( Nagata, K. and Yamada, K. M. ( 1986 ) J.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(117)

Biol.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(118)

Chem., 261, 7531 - 7536 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(119)

Both prolyl 4 - hydroxylase and HSP47 interact with procollagen alpha - chains during their folding and / or modification in the endoplasmic reticulum.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(120)

Recent study has revealed that a simple collagen model peptide, ( Pro - Pro - Gly ) n, is recognized by HSP47 as well as by prolyl 4 - hydroxylase in vitro ( Koide et al., manuscript submitted ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(121)

In the present study, we investigated the effect of HSP47 on the prolyl 4 - hydroxylation of such collagen model peptides.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(122)

To monitor the enzymatic hydroxylation of the peptides, we developed a non - RI assay system based on reversed - phase HPLC.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(123)

When HSP47 was added to the reaction mixture, substrate and less - hydroxylated materials accumulated.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(124)

This effect depended on the peptide - binding activity of HSP47, because a mutant HSP47 without collagen - binding activity did not show any inhibitory effect on prolyl 4 - hydroxylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(125)

Kinetic analysis and other biochemical analyses suggest that HSP47 retards the enzymatic reaction competing for the substrate peptide.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(126)

Mouse K - Cl cotransporter KCC1 : cloning, mapping, pathological expression, and functional regulation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(127)

Although K - Cl cotransporter ( KCC1 ) mRNA is expressed in many tissues, K - Cl cotransport activity has been measured in few cell types, and detection of endogenous KCC1 polypeptide has not yet been reported.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(128)

We have cloned the mouse erythroid KCC1 ( mKCC1 ) cDNA and its flanking genomic regions and mapped the mKCC1 gene to chromosome 8.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(129)

Three anti - peptide antibodies raised against recombinant mKCC1 function as immunoblot and immunoprecipitation reagents.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(130)

The tissue distributions of mKCC1 mRNA and protein are widespread, and mKCC1 RNA is constitutively expressed during erythroid differentiation of ES cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(131)

KCC1 polypeptide or related antigen is present in erythrocytes of multiple species in which K - Cl cotransport activity has been documented.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(132)

Erythroid KCC1 polypeptide abundance is elevated in proportion to reticulocyte counts in density - fractionated cells, in bleeding - induced reticulocytosis, in mouse models of sickle cell disease and thalassemia, and in the corresponding human disorders. mKCC1 - mediated uptake of ( 86 ) Rb into Xenopus oocytes requires extracellular Cl ( - ), is blocked by the diuretic R ( + ) - [ 2 - n - butyl - 6, 7 - dichloro - 2 - cyclopentyl - 2, 3 - dihydro - 1 - oxo - 1H - indenyl - 5 - yl - ) oxy ] acetic acid, and exhibits an erythroid pattern of acute regulation, with activation by hypotonic swelling, N - ethylmaleimide, and staurosporine and inhibition by calyculin and okadaic acid.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(133)

These reagents and findings will expedite studies of KCC1 structure - function relationships and of the pathobiology of KCC1 - mediated K - Cl cotransport.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(134)

Progesterone metabolism in the human kidney and inhibition of 11beta - hydroxysteroid dehydrogenase type 2 by progesterone and its metabolites.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(135)

Progesterone binds with high affinity to the mineralocorticoid ( MC ) receptor, but confers only very low agonistic MC activity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(136)

Therefore, progesterone is a potent MC antagonist in vitro.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(137)

Although progesterone reaches up to 100 times higher plasma levels in late pregnancy than aldosterone, the in vivo MC antagonistic effect of progesterone seems to be relatively weak.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(138)

One explanation for this phenomenon could be local metabolism of progesterone in the human kidney, similar to the inactivation of cortisol to cortisone by the 11beta - hydroxysteroid dehydrogenase ( 11beta - HSD ) type 2.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(139)

We studied the metabolism of progesterone in the human kidney in vitro and found reduction to 20alpha - dihydro ( DH ) - progesterone as the main metabolite.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(140)

Ring - A reduction to 5alpha - DH - progesterone, 20alpha - DH - 5alpha - DH - progesterone, and 3beta, 5alpha - tetrahydro ( TH ) - progesterone was also documented.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(141)

We further showed for the first time that 17 - hydroxylation of progesterone ( 17alpha - OH - progesterone, 17alpha - OH, 20alpha - DH - progesterone ), normally localized in the adrenals and the gonads, occurs in the human adult kidney.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(142)

We found no formation of deoxycorticosterone from progesterone in the human adult kidney.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(143)

Using human kidney cortex microsomes, we tested the inhibitory potency of progesterone and its metabolites on the 11beta - HSD type 2.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(144)

The most potent inhibitor was progesterone itself ( IC50 = 4. 8 x 10 ( - 8 ) mol / L ), followed by 5alpha - DH - progesterone ( IC50 = 2. 4 x 10 ( - 7 ) mol / L ), 20alpha - DH - progesterone, 3beta, 5alpha - TH - progesterone, 17alpha - OH - progesterone, and 20alpha - DH - 5alpha - DH - progesterone ( IC50 between 7. 7 x 10 ( - 7 ) mol / L and 1. 3 x 10 ( - 6 ) mol / L ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(145)

The least potent inhibitor was 17alpha - OH, 20alpha - DH - progesterone.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(146)

In addition to progesterone metabolism by the kidney, the inhibition of 11beta - HSD type 2 by progesterone and its metabolites could be a second explanation for the weak MC - antagonist activity of progesterone in vivo.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(147)

Inhibition of 11beta - HSD type 2 leads to an increase of intracellular cortisol in a way that the local equilibrium between the MC agonist cortisol and the antagonist progesterone is shifted to the agonist side.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(148)

Deglycosylation of a bifunctional lutropin - follitropin agonist reduced its follitropin activity more than its lutropin activity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(149)

OBJECTIVE : To design a drug that blocks the gonadal actions of lutropins and follitropins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(150)

DESIGN : Controlled in vitro study.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(151)

SETTING : Academic laboratory.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(152)

PATIENT ( S ) : None.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(153)

INTERVENTION ( S ) : We removed three glycosylation signals from an hCG - hFSH chimera known to have high affinity for LH and FSH receptors, expecting this would create a bifunctional antagonist ( dgCFC ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(154)

To offset the inhibition of subunit combination caused by deglycosylation of alpha - subunit loop 2, we prepared dgCFC as a single - chain fusion protein containing the alpha - subunit downstream of the chimeric beta - subunit.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(155)

MAIN OUTCOME MEASURE ( S ) : Receptor binding, cyclic adenosine monophosphate accumulation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(156)

RESULT ( S ) : dgCFC bound LH or FSH receptors similar to hCG or hFSH.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(157)

It was a partial agonist and had one tenth the efficacy of hFSH and two thirds the efficacy of hCG.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(158)

CONCLUSION ( S ) : The surprising high residual lutropin activity of dgCFC indicated that its FSH residues offset the effects of deglycosylation, suggesting this approach to preparing a bifunctional antagonist is unlikely to lead to a useful drug.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(159)

The increased lutropin efficacy of dgCFC relative to deglycosylated hCG supports the idea that oligosaccharides modulate glycoprotein hormone efficacy through an influence on hormone conformation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(160)

Regulation of thyrotropin receptor protein expression in insect cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(161)

Expression of large quantities of conformationally intact thyrotropin receptor ( TSHR ) is essential to understand the structure - function relationship of the receptor.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(162)

We expressed three different constructs of full - length human TSHR in insect cells : ( a ) a TSHR cDNA lacking signal sequence ( TSHR - ns ), ( b ) a TSHR cDNA containing human TSHR signal sequence ( TSHR - hs ) and ( c ) a TSHR cDNA with baculovirus envelope protein encoded signal sequence gp - 67 ( TSHR - gp ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(163)

No unique protein band, corresponding to any of these recombinant proteins, was visible upon Coomassie Blue staining after SDS - PAGE.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(164)

However, Western blot using TSHR specific monoclonal antibody showed unique bands around 80, 100 and 100 kDa in TSHR - ns, TSHR - hs and TSHR - gp virus infected insect cells respectively.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(165)

All three full - length TSHR proteins could neutralize the TSH binding inhibitory immunoglobulin ( TBII ) activity from sera of experimental animals.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(166)

However, only glycosylated proteins ( TSHR - hs and TSHR - gp ) neutralized the TBII activity of sera from autoimmune thyroid patients, confirming the importance of glycosylation for patient autoantibody reactivity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	However, only Glycosylationglycosylated proteins Protein( TSHR - hs and TSHR - gp ) neutralized the TBII activity of sera from autoimmune thyroid patients, confirming the importance of glycosylation for patient autoantibody reactivity.
0.9998	Methylation	However, only Glycosylationglycosylated proteins ( TSHR - hs and ProteinTSHR - gp ) neutralized the TBII activity of sera from autoimmune thyroid patients, confirming the importance of glycosylation for patient autoantibody reactivity.

(167)

Expression levels of full - length TSHR proteins were much lower than the levels of similarly produced corresponding ectodomains of TSHR proteins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(168)

Southern blot and Northern blot analyses showed that DNA and RNA levels in full - length TSHR virus infected insect cells were comparable to the levels found in cells infected with viruses encoding only the ectodomain of TSHR.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(169)

These data suggest that full - length TSHR expression is very low and is regulated at the translational level.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(170)

BasT, a membrane - bound transducer protein for amino acid detection in Halobacterium salinarum.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(171)

Halophilic archaea, such as eubacteria, use methyl - accepting chemotaxis proteins ( MCPs ) to sense their environment.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(172)

We show here that BasT is a halobacterial transducer protein ( Htp ) responsible for chemotaxis towards five attractant amino acids.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(173)

The C - terminus of the protein exhibits the highly conserved regions that are diagnostic for MCPs : the signalling domain for communication with the histidine kinase and the methylation sites that interact with the methylation / demethylation enzymes for adaptation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(174)

Hydropathy analysis predicts an enterobacterial - type transducer protein topology for BasT, with an extracellular putative ligand - binding domain flanked by two transmembrane helices and a cytoplasmic domain.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(175)

BasT - inactivated mutant cells are missing a membrane protein radiolabelled with L - [ methyl - 3H ] - methionine in wild - type cells, confirming that BasT is methylatable and membrane bound.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	BasT - inactivated mutant cells are missing a membrane protein radiolabelled with L - [ methyl - 3H ] - methionine in wild - type cells, confirming that ProteinBasT is Methylationmethylatable and membrane bound.

(176)

Behavioural analysis of the basT mutant cells by capillary and chemical - in - plug assays demonstrates complete loss of chemotactic responses towards five ( leucine, isoleucine, valine, methionine and cysteine ) of the six attractant amino acids for Halobacterium salinarum, whereas they still respond to arginine.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(177)

The volatile methyl group production assays also corroborate these findings and confirm that BasT signalling induces methyl group turnover.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(178)

Our data identify BasT as the chemotaxis transducer protein for the branched chain amino acids leucine, isoleucine and valine as well as for methionine and cysteine.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(179)

Thus, BasT and the arginine sensor Car cover the entire spectrum of chemotactic responses towards attractant amino acids in H. salinarum.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(180)

Additional N - glycosylation at Asn ( 13 ) rescues the human LHbeta - subunit from disulfide - linked aggregation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	Additional GlycosylationN - glycosylation at EntityAsn ( 13 ) rescues the human LHbeta - subunit from disulfide - linked aggregation.

(181)

CG, LH, FSH, and TSH are a family of heterodimeric glycoprotein hormones that contain a common alpha - subunit, but differ in their hormone - specific beta - subunits.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(182)

Despite the considerable homology between LHbeta and CGbeta, we previously demonstrated that, when expressed in GH ( 3 ) cells, the secreted form of LHbeta showed mispaired disulfide - linked aggregation in addition to monomer, whereas no aggregation was observed in CGbeta.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(183)

To determine the domains which are associated with the LHbeta - aggregation and which prevent CGbeta - aggregation, mutant beta - subunits in glycosylation and carboxy - terminus were expressed in GH ( 3 ) cells, and the occurrence of aggregation was assessed by continuous labeling with [ 35S ] methionine / cysteine, immunoprecipitation with anti - hCGbeta serum, and sodium dodecyl sulfate - polyacrylamide gel electrophoresis in a non - reducing condition.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(184)

No aggregation was seen when N - linked oligosaccharides were attached to the Asn ( 13 ) of LHbeta.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(185)

Removal of the carbohydrate unit at the Asn ( 13 ) of CGbeta caused aggregation, although the amount was less than 10 % of monomer.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(186)

The carboxy - terminal regions of neither LHbeta nor CGbeta were associated with their aggregation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(187)

Both CGbeta wild - type ( WT ) and CGbeta lacking N - glycosylation at Asn ( 13 ) ( CGbeta - N13 ) showed aggregates in lysate.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	Both CGbeta wild - type ( WT ) and CGbeta lacking GlycosylationN - glycosylation at EntityAsn ( 13 ) ( CGbeta - N13 ) showed aggregates in lysate.

(188)

However, in contrast to CGbeta - N13, CGbetaWT revealed no aggregation in medium.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(189)

These results indicate that the backbone structure consisting of 114 amino acids and N - linked glycosylation at Asn ( 30 ) is involved in the aggregation of LHbeta.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Relation	Text (Head - Tail)
1.0000	Methylation	These results indicate that the backbone structure consisting of 114 amino acids and GlycosylationN - linked glycosylation at EntityAsn ( 30 ) is involved in the aggregation of LHbeta.
1.0000	Methylation	These results indicate that the backbone structure consisting of 114 amino acids and N - linked Glycosylationglycosylation at EntityAsn ( 30 ) is involved in the aggregation of LHbeta.

(190)

Moreover, N - glycosylation at Asn ( 13 ) does not prevent such aggregation, but instead plays an important role in correct folding for both LHbeta - and CGbeta - subunits to be secreted as monomer.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	Moreover, GlycosylationN - glycosylation at EntityAsn ( 13 ) does not prevent such aggregation, but instead plays an important role in correct folding for both LHbeta - and CGbeta - subunits to be secreted as monomer.

(191)

Type XIII collagen forms homotrimers with three triple helical collagenous domains and its association into disulfide - bonded trimers is enhanced by prolyl 4 - hydroxylase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(192)

Type XIII collagen is a type II transmembrane protein predicted to consist of a short cytosolic domain, a single transmembrane domain, and three collagenous domains flanked by noncollagenous sequences.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(193)

Previous studies on mRNAs indicate that the structures of the collagenous domain closest to the cell membrane, COL1, the adjacent noncollagenous domain, NC2, and the C - terminal domains COL3 and NC4 are subject to alternative splicing.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(194)

In order to extend studies of type XIII collagen from cDNAs to the protein level we have produced it in insect cells by means of baculoviruses.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(195)

Type XIII collagen alpha chains were found to associate into disulfide - bonded trimers, and hydroxylation of proline residues dramatically enhanced this association.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Relation	Text (Head - Tail)
0.9999	Methylation	Type XIII collagen alpha chains were found to associate into disulfide - bonded trimers, and Hydroxylationhydroxylation of Entityproline residues dramatically enhanced this association.
0.9997	Methylation	Type XIII collagen alpha chains were found to associate into disulfide - bonded trimers, and Hydroxylationhydroxylation of Entityproline residues dramatically enhanced this association.

(196)

This protein contains altogether eight cysteine residues, and interchain disulfide bonds could be located in the NC1 domain and possibly at the junction of COL1 and NC2, while the two cysteine residues in NC4 are likely to form intrachain bonds.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(197)

Pepsin and trypsin / chymotrypsin digestions indicated that the type XIII collagen alpha chains form homotrimers whose three collagenous domains are in triple helical conformation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(198)

The thermal stabilities ( T ( m ) ) of the COL1, COL2, and COL3 domains were 38, 49 and 40 degrees C, respectively.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(199)

The T ( m ) of the central collagenous domain is unusually high, which in the light of this domain being invariant in terms of alternative splicing suggests that the central portion of the molecule may have an important role in the stability of the molecule.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(200)

All in all, most of the type XIII collagen ectodomain appears to be present in triple helical conformation, which is in clear contrast to the short or highly interrupted triple helical domains of the other known collagenous transmembrane proteins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(201)

Symmetric and asymmetric DNA methylation in the human IGF2 - H19 imprinted region.

Precision: 8.33

Recall: 100.00

F1: 15.38

Score	Relation	Text (Head - Tail)
0.9970	Methylation	Symmetric and asymmetric DNA_methylationDNA methylation in the human IGF2 - H19 Entityimprinted region .
0.9973	Methylation	Symmetric and asymmetric DNA DNA_methylationmethylation in the human IGF2 - H19 Entityimprinted region .
0.9923	Methylation	Symmetric and asymmetric DNA DNA_methylationmethylation in the human IGF2 - H19 Entityimprinted region.
0.9913	Methylation	Symmetric and asymmetric DNA_methylationDNA methylation in the human IGF2 - H19 Entityimprinted region.
0.9487	Methylation	Symmetric and asymmetric DNA_methylationDNA methylation in the human IGF2 - H19 Entityimprinted region .
0.8638	Methylation	Symmetric and asymmetric DNA_methylationDNA methylation in the human IGF2 - H19 Entityimprinted region.
0.6757	Methylation	Symmetric and asymmetric DNA_methylationDNA DNA_methylationmethylation in the human IGF2 - H19 imprinted region.
0.6489	Methylation	Symmetric and asymmetric DNA_methylationDNA DNA_methylationDNA methylation in the human IGF2 - H19 imprinted region.
0.6317	Methylation	Symmetric and asymmetric DNA_methylationDNA methylation DNA_methylationmethylation in the human IGF2 - H19 imprinted region.
0.5755	Methylation	Symmetric and asymmetric DNA_methylationDNA methylation DNA_methylationmethylation in the human IGF2 - H19 imprinted region.
0.4911	Methylation	Symmetric and asymmetric DNA_methylationDNA methylation DNA_methylationDNA methylation in the human IGF2 - H19 imprinted region.
0.4623	Methylation	Symmetric and asymmetric DNA_methylationDNA DNA_methylationmethylation in the human IGF2 - H19 imprinted region.

(202)

The two contiguous IGF2 ( human insulin - like growth factor II ) and H19 genes are reciprocally imprinted in both human and mouse.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(203)

In most tissues, IGF2 is transcribed only from the paternal chromosome while H19 is transcribed only from the maternal allele.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(204)

The presence of a differential methylation region ( DMR ) on the two parental alleles at the 5'flanking region of H19 has been proposed to constitute the gametic imprint, which controls the reciprocal allelic expression of the two genes.

Precision: 8.33

Recall: 100.00

F1: 15.38

Score	Relation	Text (Head - Tail)
0.9981	Methylation	The presence of a differential DNA_methylationmethylation region ( DMR ) on the two parental alleles at the Entity5'flanking region of H19 has been proposed to constitute the gametic imprint, which controls the reciprocal allelic expression of the two genes.
0.9980	Methylation	The presence of a differential DNA_methylationmethylation region ( DMR ) on the two parental alleles at the Entity5'flanking region of H19 has been proposed to constitute the gametic imprint, which controls the reciprocal allelic expression of the two genes.
0.9970	Methylation	The presence of a differential DNA_methylationmethylation region ( DMR ) on the two parental alleles at the Entity5 ' flanking region of H19 has been proposed to constitute the gametic imprint, which controls the reciprocal allelic expression of the two genes.
0.9970	Methylation	The presence of a differential DNA_methylationmethylation region ( DMR ) on the two parental alleles at the 5 ' Entityflanking region of H19 has been proposed to constitute the gametic imprint, which controls the reciprocal allelic expression of the two genes.
0.9968	Methylation	The presence of a differential DNA_methylationmethylation region ( DMR ) on the two parental alleles at the 5 ' Entityflanking region of H19 has been proposed to constitute the gametic imprint, which controls the reciprocal allelic expression of the two genes.
0.5844	Methylation	The presence of a differential methylation region ( DMR ) on the two parental alleles at the 5 ' Entityflanking Entityflanking region of H19 has been proposed to constitute the gametic imprint, which controls the reciprocal allelic expression of the two genes.
0.5536	Methylation	The presence of a differential methylation region ( DMR ) on the two parental alleles at the Entity5'flanking region Entityflanking region of H19 has been proposed to constitute the gametic imprint, which controls the reciprocal allelic expression of the two genes.
0.5433	Methylation	The presence of a differential methylation region ( DMR ) on the two parental alleles at the Entity5'flanking Entityflanking region of H19 has been proposed to constitute the gametic imprint, which controls the reciprocal allelic expression of the two genes.
0.4431	Methylation	The presence of a differential methylation region ( DMR ) on the two parental alleles at the Entity5 ' Entityflanking region of H19 has been proposed to constitute the gametic imprint, which controls the reciprocal allelic expression of the two genes.
0.4217	Methylation	The presence of a differential methylation region ( DMR ) on the two parental alleles at the 5 ' Entityflanking region Entityflanking region of H19 has been proposed to constitute the gametic imprint, which controls the reciprocal allelic expression of the two genes.
0.4042	Methylation	The presence of a differential methylation region ( DMR ) on the two parental alleles at the Entity5'flanking region Entityflanking region of H19 has been proposed to constitute the gametic imprint, which controls the reciprocal allelic expression of the two genes.
0.4023	Methylation	The presence of a differential methylation region ( DMR ) on the two parental alleles at the Entity5'flanking region Entityflanking region of H19 has been proposed to constitute the gametic imprint, which controls the reciprocal allelic expression of the two genes.

(205)

Using bisulfite genomic sequencing, we have assessed the methylation status of cytosine ( including 154 CpG sites ) in six CpG - rich regions of the human IGF2 - H19 genes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9997	Methylation	Using bisulfite genomic sequencing, we have assessed the DNA_methylationmethylation status of Entitycytosine ( including 154 CpG sites ) in six CpG - rich regions of the human IGF2 - H19 genes.

(206)

In a CpG island near promoter P3 of the IGF2 gene, more than 99. 8 % of all cytosines were converted to thymidine by sodium bisulfite mutagenesis, indicating that none of the CpGs was methylated.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	In a CpG island near promoter P3 of the IGF2 gene, more than 99. 8 % of all cytosines were converted to thymidine by sodium bisulfite mutagenesis, indicating that none of the EntityCpGs was DNA_methylationmethylated .

(207)

In the IGF2 exon 8 - 9 region, mosaic methylation of 56 CpG sites was observed in fetal tissues and in adult blood DNA.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Relation	Text (Head - Tail)
0.9999	Methylation	In the IGF2 exon 8 - 9 region, mosaic DNA_methylationmethylation of 56 EntityCpG sites was observed in fetal tissues and in adult blood DNA.
0.9997	Methylation	In the IGF2 exon 8 - 9 region, mosaic DNA_methylationmethylation of 56 EntityCpG sites was observed in fetal tissues and in adult blood DNA.

(208)

In contrast to the mosaic methylation of IGF2, the allelic methylation of the human H19 DMR was uniform.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	In contrast to the mosaic DNA_methylationmethylation of ProteinIGF2 , the allelic methylation of the human H19 DMR was uniform.
0.9959	Methylation	In contrast to the mosaic methylation of IGF2, the allelic DNA_methylationmethylation of the human H19 EntityDMR was uniform.

(209)

In the CpG region located 2 kb upstream ( - 2362 to - 1911 ) of the H19 transcription site, all 25 CpG sites were completely methylated on only one parental allele.

Precision: 20.00

Recall: 100.00

F1: 33.33

Score	Relation	Text (Head - Tail)
1.0000	Methylation	In the CpG region located 2 kb upstream ( - 2362 to - 1911 ) of the H19 transcription site, all Entity25 CpG sites were completely DNA_methylationmethylated on only one parental allele.
1.0000	Methylation	In the CpG region located 2 kb upstream ( - 2362 to - 1911 ) of the H19 transcription site, all 25 EntityCpG sites were completely DNA_methylationmethylated on only one parental allele.
0.9982	Methylation	In the CpG region located 2 kb upstream ( - 2362 to - 1911 ) of the H19 transcription site, all Entity25 CpG sites were completely DNA_methylationmethylated on only one parental allele.
0.9975	Methylation	In the CpG region located 2 kb upstream ( - 2362 to - 1911 ) of the H19 transcription site, all Entity25 CpG sites were completely DNA_methylationmethylated on only one parental allele.
0.9974	Methylation	In the CpG region located 2 kb upstream ( - 2362 to - 1911 ) of the H19 transcription site, all 25 EntityCpG sites were completely DNA_methylationmethylated on only one parental allele.

(210)

Uniform allele - specific methylation was also observed in the CpG island proximal to the H19 promoter ( - 711 to - 290 ) with complete methylation of all 25 CpG sites in one parental allele.

Precision: 33.33

Recall: 50.00

F1: 40.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	Uniform allele - specific DNA_methylationmethylation was also observed in the EntityCpG island proximal to the H19 promoter ( - 711 to - 290 ) with complete methylation of all 25 CpG sites in one parental allele.
0.9999	Methylation	Uniform allele - specific methylation was also observed in the CpG island proximal to the H19 promoter ( - 711 to - 290 ) with complete DNA_methylationmethylation of all 25 EntityCpG sites in one parental allele.
0.9999	Methylation	Uniform allele - specific methylation was also observed in the CpG island proximal to the H19 promoter ( - 711 to - 290 ) with DNA_methylationcomplete methylation of all 25 EntityCpG sites in one parental allele.
	Methylation	Uniform allele - specific methylation was also observed in the CpG island proximal to the H19 promoter ( - 711 to - 290 ) with complete DNA_methylationmethylation of all Entity25 CpG sites in one parental allele.

(211)

In contrast, the CpG region in the H19 promoter ( - 292 to + 15 ) was mosaically methylated in all tissues.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Relation	Text (Head - Tail)
0.9989	Methylation	In contrast, the EntityCpG region in the H19 promoter ( - 292 to + 15 ) was mosaically DNA_methylationmethylated in all tissues.
0.9930	Methylation	In contrast, the EntityCpG region in the H19 promoter ( - 292 to + 15 ) was mosaically DNA_methylationmethylated in all tissues.

(212)

In addition, cytosine was methylated at three CpNpG and GpNpC sites on the top DNA strand and one CpNpG site on the bottom DNA strand from the fetal brain.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	In addition, cytosine was DNA_methylationmethylated at three EntityCpNpG and GpNpC sites on the top DNA strand and one CpNpG site on the bottom DNA strand from the fetal brain.
0.9997	Methylation	In addition, cytosine was DNA_methylationmethylated at three CpNpG and EntityGpNpC sites on the top DNA strand and one CpNpG site on the bottom DNA strand from the fetal brain.
0.9988	Methylation	In addition, cytosine was DNA_methylationmethylated at three CpNpG and GpNpC sites on the top DNA strand and one EntityCpNpG site on the bottom DNA strand from the fetal brain.

(213)

The cytosines at CpG sites were methylated on both DNA strands ( symmetric methylation ) while cytosines at the CpNpG and GpNpC sites were methylated on only one DNA strand ( asymmetric methylation ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9987	Methylation	The cytosines at CpG sites were methylated on both DNA strands ( symmetric methylation ) while Entitycytosines at the CpNpG and GpNpC sites were DNA_methylationmethylated on only one DNA strand ( asymmetric methylation ).
0.9955	Methylation	The Entitycytosines at CpG sites were DNA_methylationmethylated on both DNA strands ( symmetric methylation ) while cytosines at the CpNpG and GpNpC sites were methylated on only one DNA strand ( asymmetric methylation ).

(214)

The asymmetric methylation was associated with tissue - specific disruption of H19 genomic imprinting in fetal brain.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9992	Methylation	The asymmetric DNA_methylationmethylation was associated with tissue - specific disruption of ProteinH19 genomic imprinting in fetal brain.

(215)

An increase in histone acetylation and IL - 2 antagonizing the immunoinhibitory effect are necessary for augmentation by butyrate of in vitro anti - TNP antibody production.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9985	Methylation	An increase in Proteinhistone Acetylationacetylation and IL - 2 antagonizing the immunoinhibitory effect are necessary for augmentation by butyrate of in vitro anti - TNP antibody production.

(216)

We investigated the role of histone acetylation in the promotion of antigen - specific antibody production in murine B cells induced by sodium butyrate ( NaBu ) plus interleukin 2 ( IL - 2 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9997	Methylation	We investigated the role of Proteinhistone Acetylationacetylation in the promotion of antigen - specific antibody production in murine B cells induced by sodium butyrate ( NaBu ) plus interleukin 2 ( IL - 2 ).

(217)

NaBu dose dependently increased the acetylation levels of histone H4 at concentrations which effectively enhanced anti - trinitrophenyl ( TNP ) antibody production in the presence of IL - 2.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	NaBu dose dependently increased the Acetylationacetylation levels of Proteinhistone H4 at concentrations which effectively enhanced anti - trinitrophenyl ( TNP ) antibody production in the presence of IL - 2.

(218)

Among other short - chain fatty acids and NaBu analogs, propionate, valerate and vinylacetate were effective in the presence of IL - 2 in increasing both antibody production and the histone H4 acetylation level, but acetate, alpha -, beta - and gamma - hydroxybutyrates and alpha -, beta - and gamma - aminobutyrates were not effective, even in the presence of IL - 2.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9980	Methylation	Among other short - chain fatty acids and NaBu analogs, propionate, valerate and vinylacetate were effective in the presence of IL - 2 in increasing both antibody production and the Proteinhistone H4 Acetylationacetylation level, but acetate, alpha -, beta - and gamma - hydroxybutyrates and alpha -, beta - and gamma - aminobutyrates were not effective, even in the presence of IL - 2.

(219)

The effect of the specific histone deacetylase inhibitor trichostatin A ( TSA ), which enhances anti - TNP antibody production without IL - 2, was markedly inhibited by adding NaBu simultaneously.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(220)

However, the effect of TSA was neither inhibited nor potentiated by NaBu in the presence of IL - 2.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(221)

Splenic B cells treated with NaBu, TSA and both together in the presence or absence of IL - 2 showed almost the same increased acetylation level of histone H4.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	Splenic B cells treated with NaBu, TSA and both together in the presence or absence of IL - 2 showed almost the same increased Acetylationacetylation level of Proteinhistone H4 .

(222)

These results suggest that the NaBu - induced enhancement of anti - TNP antibody production in the presence of IL - 2 is mediated through a moderate increase in the level of histone acetylation and that NaBu has both stimulating and inhibiting activities for anti - TNP antibody production, the latter of which is overcome by IL - 2.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9989	Methylation	These results suggest that the NaBu - induced enhancement of anti - TNP antibody production in the presence of IL - 2 is mediated through a moderate increase in the level of Proteinhistone Acetylationacetylation and that NaBu has both stimulating and inhibiting activities for anti - TNP antibody production, the latter of which is overcome by IL - 2.

(223)

New immunofluorescence assays for detection of Human herpesvirus 8 - specific antibodies.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(224)

Several assays have been developed for detection of immunoglobulin G antibodies to Human herpesvirus 8 ( HHV - 8 ), including immunofluorescence assays ( IFAs ) and enzyme - linked immunosorbent assays ( ELISAs ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(225)

However, the specificity and sensitivity of these assays are not completely defined due to the lack of a " gold standard. " Although IFAs based on primary effusion lymphoma ( PEL ) cell lines are used widely, the assays can be confounded by nonspecific reactions against cellular components and potential cross - reaction with antibodies against other herpesviruses.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(226)

To provide more reliable IFAs, we established recombinant Semliki Forest viruses ( rSFVs ) expressing the HHV - 8 - specific proteins ORF73 and K8. 1 and used BHK - 21 cells infected with these rSFVs for IFA ( ORF73 - IFA and K8. 1 - IFA ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(227)

Expression of the HHV - 8 - specific proteins at very high levels by the rSFV system allowed easy scoring for IFA and thereby increased specificity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(228)

The rSFV system also allowed detection of antibodies against glycosylation - dependent epitopes of K8. 1.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(229)

Titers measured by rSFV - based IFAs and PEL - based IFAs correlated well ( correlation coefficients of > 0. 9 ), and concordances of seroreactivities between rSFV - based and PEL - based IFAs were > 97 % ( kappa > 0. 93 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(230)

K8. 1 - IFA was more sensitive than either ORF73 - IFA or peptide ELISAs.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(231)

Using PEL - based lytic IFA as a reference assay, the sensitivity and specificity of K8. 1 - IFA were estimated to be 94 and 100 %, respectively.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(232)

HHV - 8 prevalences determined by K8. 1 - IFA among the human immunodeficiency virus ( HIV ) - positive ( HIV ( + ) ) Kaposi's sarcoma ( KS ) patients, HIV ( + ) KS ( - ) patients, and healthy controls were 100, 65, and 6. 7 %, respectively, which were consistent with prior reports.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(233)

Therefore, our rSFV - based IFAs may provide a specific and sensitive method for use in epidemiology studies.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(234)

In addition, they will provide a basis for further development of diagnostic tests for HHV - 8 infection.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(235)

Evidence that the lizard helospectin peptides are O - glycosylated.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9994	Methylation	Evidence that the lizard Proteinhelospectin peptides are GlycosylationO - glycosylated .

(236)

Six forms of helospectin ( a vasoactive intestinal peptide analogue ) were purified from the venom of the Heloderma horridum lizard.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(237)

Their identification was performed by combining sequencing by automated Edman degradation and electrospray mass spectrometry analysis on the complete peptides and their tryptic fragments.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(238)

The products resulting from the action of an O - glycosidase were also analysed.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(239)

Two forms were identified as the previously named Hs1 and Hs2 of 38 and 37 amino - acid residues, respectively.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(240)

Two forms corresponded to Hs1 and Hs2 O - glycosylated by a N - acetylhexosamine - hexose motif attached to the Ser32 residue.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(241)

Two other forms were not completely characterized but might correspond to the O - glycosylated forms bearing a phosphate or a sulfate group.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(242)

The glycosylation did not affect the capacity of the helospectins to recognize and to activate the human and the rat VPAC1 and VPAC2 receptors.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Relation	Text (Head - Tail)
0.9980	Methylation	The Glycosylationglycosylation did not affect the capacity of the Proteinhelospectins to recognize and to activate the human and the rat VPAC1 and VPAC2 receptors.

(243)

Changes in methionine adenosyltransferase and S - adenosylmethionine homeostasis in alcoholic rat liver.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(244)

Liver - specific and non - liver - specific methionine adenosyltransferase ( MAT ) are products of two genes, MAT1A and MAT2A, respectively, that catalyze the formation of S - adenosylmethionine ( SAM ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(245)

We previously showed that MAT2A expression was associated with more rapid cell growth.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(246)

Changes in MAT expression have not been examined in animal models of alcoholic liver injury, which is the focus of the current study.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(247)

After rats were fed intragastrically with ethanol and high fat for 9 wk, the mRNA level of both MAT forms doubled but only the protein level of MAT2A increased.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(248)

Although liver - specific MAT activity did not change, it was 32 % lower after one and 68 % lower after eight weekly enteral doses of lipopolysaccharide.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(249)

Hepatic levels of methionine, SAM, and DNA methylation fell by approximately 40 %. c - myc was hypomethylated, and its mRNA level increased.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	Hepatic levels of methionine, SAM, and DNA methylation fell by approximately 40 %. Proteinc - myc was DNA_methylationhypomethylated , and its mRNA level increased.

(250)

Genome - wide DNA strand break increased.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(251)

Thus in the prefibrotic stage of alcoholic liver injury, there is already a switch in MAT expression, global DNA hypomethylation, increased c - myc expression, and genome - wide DNA strand break.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(252)

These changes may be important in predisposing this liver disease to malignant degeneration.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(253)

Hsl7p, the yeast homologue of human JBP1, is a protein methyltransferase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(254)

The yeast protein Hsl7p is a homologue of Janus kinase binding protein 1, JBP1, a newly characterized protein methyltransferase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(255)

In this report, Hsl7p also is shown to be a methyltransferase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(256)

It can be crosslinked to [ ( 3 ) H ] S - adenosylmethionine and exhibits in vitro protein methylation activity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(257)

Calf histones H2A and H4 and bovine myelin basic protein were methylated by Hsl7p, whereas histones H1, H2B, and H3 and bovine cytochrome c were not.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	Calf histones H2A and H4 and bovine Proteinmyelin basic protein were Catalysismethylated by Hsl7p, whereas histones H1, H2B, and H3 and bovine cytochrome c were not.
1.0000	Methylation	Calf histones H2A and H4 and bovine myelin basic protein were Catalysismethylated by ProteinHsl7p , whereas histones H1, H2B, and H3 and bovine cytochrome c were not.
0.9997	Methylation	Calf histones H2A and H4 and bovine myelin basic protein were Catalysismethylated by Hsl7p, whereas histones H1, ProteinH2B , and H3 and bovine cytochrome c were not.
0.9997	Methylation	Calf histones H2A and H4 and bovine myelin basic protein were Catalysismethylated by Hsl7p, whereas Proteinhistones H1 , H2B, and H3 and bovine cytochrome c were not.
0.9995	Methylation	Calf histones H2A and H4 and bovine myelin basic protein were Catalysismethylated by Hsl7p, whereas histones H1, H2B, and ProteinH3 and bovine cytochrome c were not.
0.9993	Methylation	Calf histones H2A and ProteinH4 and bovine myelin basic protein were Catalysismethylated by Hsl7p, whereas histones H1, H2B, and H3 and bovine cytochrome c were not.
0.9992	Methylation	Calf histones H2A and H4 and bovine myelin basic protein were Catalysismethylated by Hsl7p, whereas histones H1, H2B, and H3 and bovine Proteincytochrome c were not.
0.9974	Methylation	Calf Proteinhistones H2A and H4 and bovine myelin basic protein were Catalysismethylated by Hsl7p, whereas histones H1, H2B, and H3 and bovine cytochrome c were not.
	Methylation	Calf histones H2A and H4 and bovine myelin basic protein were Methylationmethylated by Hsl7p, whereas histones H1, ProteinH2B , and H3 and bovine cytochrome c were not.
	Methylation	Calf histones H2A and H4 and bovine myelin basic protein were Methylationmethylated by Hsl7p, whereas histones H1, H2B, and H3 and bovine Proteincytochrome c were not.
	Methylation	Calf histones H2A and H4 and bovine myelin basic protein were Methylationmethylated by Hsl7p, whereas Proteinhistones H1 , H2B, and H3 and bovine cytochrome c were not.
	Methylation	Calf Proteinhistones H2A and H4 and bovine myelin basic protein were Methylationmethylated by Hsl7p, whereas histones H1, H2B, and H3 and bovine cytochrome c were not.
	Methylation	Calf histones H2A and H4 and bovine myelin basic protein were Methylationmethylated by ProteinHsl7p , whereas histones H1, H2B, and H3 and bovine cytochrome c were not.
	Methylation	Calf histones H2A and H4 and bovine myelin basic protein were Methylationmethylated by Hsl7p, whereas histones H1, H2B, and ProteinH3 and bovine cytochrome c were not.
	Methylation	Calf histones H2A and H4 and bovine Proteinmyelin basic protein were Methylationmethylated by Hsl7p, whereas histones H1, H2B, and H3 and bovine cytochrome c were not.
	Methylation	Calf histones H2A and ProteinH4 and bovine myelin basic protein were Methylationmethylated by Hsl7p, whereas histones H1, H2B, and H3 and bovine cytochrome c were not.

(258)

We demonstrated that JBP1 can complement Saccharomyces cerevisiae with a disrupted HSL7 gene as judged by a reduction of the elongated bud phenotype, and a point mutation in the JBP1 S - adenosylmethionine consensus binding sequence eliminated all complementation by JBP1.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(259)

Therefore, we conclude the yeast protein Hsl7p is a sequence and functional homologue of JBP1.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(260)

These data provide evidence for an intricate link between protein methylation and macroscopic changes in yeast morphology.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(261)

Rapid induction of histone hyperacetylation and cellular differentiation in human breast tumor cell lines following degradation of histone deacetylase - 1.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9998	Methylation	Rapid induction of Proteinhistone Acetylationhyperacetylation and cellular differentiation in human breast tumor cell lines following degradation of histone deacetylase - 1.

(262)

Quinidine inhibits proliferation and promotes cellular differentiation in human breast tumor epithelial cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(263)

Previously we showed quinidine arrested MCF - 7 cells in G ( 1 ) phase of the cell cycle and led to a G ( 1 ) to G ( 0 ) transition followed by apoptotic cell death.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(264)

The present experiments demonstrated that MCF - 7, MCF - 7ras, T47D, MDA - MB - 231, and MDA - MB - 435 cells transiently differentiate before undergoing apoptosis in response to quinidine.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(265)

The cells accumulated lipid droplets, and the cytokeratin 18 cytoskeleton was reorganized.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(266)

Hyperacetylated histone H4 appeared within 2 h of the addition of quinidine to the medium, and levels were maximal by 24 h.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9985	Methylation	AcetylationHyperacetylated Proteinhistone H4 appeared within 2 h of the addition of quinidine to the medium, and levels were maximal by 24 h.

(267)

Quinidine - treated MCF - 7 cells showed elevated p21 ( WAF1 ), hypophosphorylation and suppression of retinoblastoma protein, and down - regulation of cyclin D1, similar to the cell cycle response observed with cells induced to differentiate by histone deacetylase inhibitors, trichostatin A, and trapoxin.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	Quinidine - treated MCF - 7 cells showed elevated Proteinp21 ( WAF1 ) , Phosphorylationhypophosphorylation and suppression of retinoblastoma protein, and down - regulation of cyclin D1, similar to the cell cycle response observed with cells induced to differentiate by histone deacetylase inhibitors, trichostatin A, and trapoxin.

(268)

Quinidine did not show evidence for direct inhibition of histone deacetylase enzymatic activity in vitro.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(269)

HDAC1 was undetectable in MCF - 7 cells 30 min after addition of quinidine to the growth medium.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(270)

The proteasome inhibitors MG - 132 and lactacystin completely protected HDAC1 from the action of quinidine.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(271)

We conclude that quinidine is a breast tumor cell differentiating agent that causes the loss of HDAC1 via a proteasomal sensitive mechanism.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(272)

Glycosylation influences gating and pH sensitivity of I ( sK ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(273)

The KvLQT1 and minK subunits that coassemble to form I ( sK ) channels, contain potential N - glycosylation sites.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(274)

To examine the role of glycosylation in channel function, a Chinese hamster ovary cell line deficient in glycosylation ( Lec - 1 ) and its parental cell line ( Pro - 5 ) were transiently transfected with human KvLQT1 ( hKvLQT1 ) cDNA, alone and in combination with the rat ( rminK ) or human minK ( hminK ) cDNA.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(275)

Functional KvLQT1 and I ( sK ) currents were expressed in both cell lines, although amplitudes were larger in Pro - 5 than Lec - 1 cells transfected with hKvLQT1 and hKvLQT1 / hminK.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(276)

For I ( sK ), but not KvLQT1, the voltage - dependence of activation was shifted to more positive voltages and the activation kinetics were slower in the Lec - 1 compared to the Pro - 5 cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(277)

The effect of extracellular acidification on recombinant KvLQT1 and I ( sK ) currents was investigated in Pro - 5 and Lec - 1 cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(278)

Changing external pH ( pH ( o ) ) from 7. 4 to 6. 0 significantly decreased the amplitude and increased the half - activation voltage ( V ( 1 / 2 ) ) of KvLQT1 currents in Pro - 5 and Lec - 1 cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(279)

In Pro - 5 cells, decreasing pH ( o ) reduced I ( sK ) amplitude without increasing V ( 1 / 2 ), whether rminK or hminK was coexpressed with hKvLQT.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(280)

In contrast, changing pH ( o ) from 7. 4 to 6. 0 did not significantly change I ( sK ) amplitude in Lec - 1 cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(281)

Thus, oligosaccharides attached to the minK subunit affect not only the gating properties, but also the pH sensitivity of I ( sK ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9921	Methylation	Thus, Entityoligosaccharides Glycosylationattached to the minK subunit affect not only the gating properties, but also the pH sensitivity of I ( sK ).

(282)

A novel post - translational modification of yeast elongation factor 1A. Methylesterification at the C terminus.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	A novel post - translational modification of yeast elongation factor 1A. MethylationMethylesterification at the C Entityterminus .
1.0000	Methylation	A novel post - translational modification of yeast elongation factor 1A. MethylationMethylesterification at the C Entityterminus.
0.9999	Methylation	A novel post - translational modification of yeast elongation factor 1A. MethylationMethylesterification at the C terminus Entity.
	Methylation	A novel post - translational modification of yeast elongation factor 1A. Methylesterification Methylationat the C Entityterminus.

(283)

Protein methylation reactions can play important roles in cell physiology.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(284)

After labeling intact Saccharomyces cerevisiae cells with S - adenosyl - l - [ methyl - ( 3 ) H ] methionine, we identified a major methylated 49 - kDa polypeptide containing [ ( 3 ) H ] methyl groups in two distinct types of linkages.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(285)

Peptide sequence analysis of the purified methylated protein revealed that it is eukaryotic elongation factor 1A ( eEF1A, formerly EF - 1alpha ), the protein that forms a complex with GTP and aminoacyl - tRNAs for binding to the ribosomal A site during protein translation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(286)

Previous studies have shown that eEF1A is methylated on several internal lysine residues to give mono -, di -, and tri - N - epsilon - methyl - lysine derivatives.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Relation	Text (Head - Tail)
0.9999	Methylation	Previous studies have shown that eEF1A is Methylationmethylated on several internal Entitylysine residues to give mono -, di -, and tri - N - epsilon - methyl - lysine derivatives.
0.9996	Methylation	Previous studies have shown that eEF1A is Methylationmethylated on several internal Entitylysine residues to give mono -, di -, and tri - N - epsilon - methyl - lysine derivatives.

(287)

We confirm this finding but also detect methylation that is released as volatile methyl groups after base hydrolysis, characteristic of ester linkages.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(288)

In cycloheximide - treated cells, methyl esterified eEF1A was detected largely in the ribosome and polysome fractions ; little or no methylated protein was found in the soluble fraction.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Relation	Text (Head - Tail)
0.9999	Methylation	In cycloheximide - treated cells, Methylationmethyl esterified ProteineEF1A was detected largely in the ribosome and polysome fractions ; little or no methylated protein was found in the soluble fraction.
0.9992	Methylation	In cycloheximide - treated cells, methyl esterified ProteineEF1A was detected largely in the ribosome and polysome fractions ; little or no Methylationmethylated protein was found in the soluble fraction.
0.9999	Methylation	In cycloheximide - treated cells, methyl Methylationesterified ProteineEF1A was detected largely in the ribosome and polysome fractions ; little or no methylated protein was found in the soluble fraction.
0.9999	Methylation	In cycloheximide - treated cells, Methylationmethyl esterified ProteineEF1A was detected largely in the ribosome and polysome fractions ; little or no methylated protein was found in the soluble fraction.

(289)

Because the base - labile, volatile [ methyl - ( 3 ) H ] radioactivity of eEF1A could be released by trypsin treatment but not by carboxypeptidase Y or chymotrypsin treatment, we suggest that the methyl ester is present on the alpha - carboxyl group of its C - terminal lysine residue.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(290)

From the results of pulse - chase experiments using radiolabeled intact yeast cells, we find that the N - methylated lysine residues of eEF1A are stable over 4 h, whereas the eEF1A carboxyl methyl ester has a half - life of less than 10 min.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Relation	Text (Head - Tail)
0.9979	Methylation	From the results of pulse - chase experiments using radiolabeled intact yeast cells, we find that the MethylationN - methylated Entitylysine residues of eEF1A are stable over 4 h, whereas the eEF1A carboxyl methyl ester has a half - life of less than 10 min.
0.9982	Methylation	From the results of pulse - chase experiments using radiolabeled intact yeast cells, we find that the MethylationN - methylated Entitylysine residues of eEF1A are stable over 4 h, whereas the eEF1A carboxyl methyl ester has a half - life of less than 10 min.

(291)

The rapid turnover of the methyl ester suggests that the methylation / demethylation of eEF1A at the C - terminal carboxyl group may represent a novel mode of regulation of the activity of this protein in yeast.

Precision: 33.33

Recall: 100.00

F1: 50.00

Score	Relation	Text (Head - Tail)
0.9987	Methylation	The rapid turnover of the methyl ester suggests that the Methylationmethylation / demethylation of eEF1A at the EntityC - terminal carboxyl group may represent a novel mode of regulation of the activity of this protein in yeast.
0.9988	Methylation	The rapid turnover of the methyl ester suggests that the Methylationmethylation / demethylation of eEF1A at the EntityC - terminal carboxyl group may represent a novel mode of regulation of the activity of this protein in yeast.
0.9987	Methylation	The rapid turnover of the methyl ester suggests that the Methylationmethylation / demethylation of eEF1A at the EntityC - terminal carboxyl group may represent a novel mode of regulation of the activity of this protein in yeast.

(292)

Decreased UDP - GlcNAc levels abrogate proliferation control in EMeg32 - deficient cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(293)

The hexosamine pathway provides UDP - N : - acetylhexosamine donor substrates used in cytosolic and Golgi - mediated glycosylation of proteins and for formation of glycosylphosphatidylinositol ( GPI ) anchors, which tether proteins to the outer plasma membrane.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(294)

We have recently identified the murine glucosamine - 6 - phosphate ( GlcN6P ) acetyltransferase, EMeg32, as a developmentally regulated enzyme on the route to UDP - N : - acetylglucosamine ( UDP - GlcNAc ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(295)

Here we describe embryos and cells that have the EMeg32 gene inactivated by homologous recombination.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(296)

Homozygous mutant embryos die at around embryonic day ( E ) 7. 5 with a general proliferative delay of development.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(297)

In vitro differentiated EMeg32 ( - / - ) ES cells show reduced proliferation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(298)

Mouse embryonic fibroblasts ( MEFs ) deficient for EMeg32 exhibit defects in proliferation and adhesiveness, which could be complemented by stable re - expression of EMeg32 or by nutritional restoration of intracellular UDP - GlcNAc levels.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(299)

Reduced UDP - GlcNAc levels predominantly translated into decreased O - GlcNAc modifications of cytosolic and nuclear proteins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(300)

Interestingly, growth - impaired EMeg32 ( - / - ) MEFs withstand a number of apoptotic stimuli and express activated PKB / AKT.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(301)

Thus, EMeg32 - dependent UDP - GlcNAc levels influence cell cycle progression and susceptibility to apoptotic stimuli.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(302)

Post - translational hydroxylation at the N - terminus of the prion protein reveals presence of PPII structure in vivo.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	Post - translational Hydroxylationhydroxylation at the EntityN - terminus of the prion protein reveals presence of PPII structure in vivo.

(303)

The transmissible spongiform encephalopathies are characterized by conversion of a host protein, PrP ( C ) ( cellular prion protein ), to a protease - resistant isoform, PrP ( Sc ) ( prion protein scrapie isoform ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(304)

The importance of the highly flexible, N - terminal region of PrP has recently become more widely appreciated, particularly the biological activities associated with its metal ion - binding domain and its potential to form a poly ( L - proline ) II ( PPII ) helix.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(305)

Circular dichroism spectroscopy of an N - terminal peptide, PrP ( 37 - 53 ), showed that the PPII helix is formed in aqueous buffer ; as it also contains an Xaa - Pro - Gly consensus sequence, it may act as a substrate for the collagen - modifying enzyme prolyl 4 - hydroxylase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(306)

Direct evidence for this modification was obtained by mass spectrometry and Edman sequencing in recombinant mouse PrP secreted from stably transfected Chinese hamster ovary cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(307)

Almost complete conversion of proline to 4 - hydroxyproline occurs specifically at residue Pro44 of this murine protein ; the same hydroxylated residue was detected, at lower levels, in PrP ( Sc ) from the brains of scrapie - infected mice.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9998	Methylation	Almost complete Hydroxylationconversion of proline to 4 - hydroxyproline occurs specifically at residue EntityPro44 of this murine protein ; the same hydroxylated residue was detected, at lower levels, in PrP ( Sc ) from the brains of scrapie - infected mice.
0.9960	Methylation	Almost complete conversion of proline to 4 - hydroxyproline occurs specifically at residue EntityPro44 of this murine protein ; the same Hydroxylationhydroxylated residue was detected, at lower levels, in PrP ( Sc ) from the brains of scrapie - infected mice.

(308)

Cation binding and / or post - translational hydroxylation of this region of PrP may regulate its role in the physiology and pathobiology of the cell.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	Cation binding and / or post - translational Hydroxylationhydroxylation of this region of ProteinPrP may regulate its role in the physiology and pathobiology of the cell.

(309)

Carboxymethylation of the PP2A catalytic subunit in Saccharomyces cerevisiae is required for efficient interaction with the B - type subunits Cdc55p and Rts1p.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(310)

Protein phosphatase 2A ( PP2A ) is an essential eukaryotic serine / threonine phosphatase known to play important roles in cell cycle regulation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(311)

Association of different B - type targeting subunits with the heterodimeric core ( A / C ) enzyme is known to be an important mechanism of regulating PP2A activity, substrate specificity, and localization.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(312)

However, how the binding of these targeting subunits to the A / C heterodimer might be regulated is unknown.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(313)

We have used the budding yeast Saccharomyces cerevisiae as a model system to investigate the hypothesis that covalent modification of the C subunit ( Pph21p / Pph22p ) carboxyl terminus modulates PP2A complex formation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(314)

Two approaches were taken.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(315)

First, S. cerevisiae cells were generated whose survival depended on the expression of different carboxyl - terminal Pph21p mutants.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(316)

Second, the major S. cerevisiae methyltransferase ( Ppm1p ) that catalyzes the methylation of the PP2A C subunit carboxyl - terminal leucine was identified, and cells deleted for this methyltransferase were utilized for our studies.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(317)

Our results demonstrate that binding of the yeast B subunit, Cdc55p, to Pph21p was disrupted by either acidic substitution of potential carboxyl - terminal phosphorylation sites on Pph21p or by deletion of the gene for Ppm1p.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(318)

Loss of Cdc55p association was accompanied in each case by a large reduction in binding of the yeast A subunit, Tpd3p, to Pph21p.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(319)

Moreover, decreased Cdc55p and Tpd3p binding invariably resulted in nocodazole sensitivity, a known phenotype of CDC55 or TPD3 deletion.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(320)

Furthermore, loss of methylation also greatly reduced the association of another yeast B - type subunit, Rts1p.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Relation	Text (Head - Tail)
0.9751	Methylation	Furthermore, loss of DNA_methylationmethylation also greatly reduced the association of another yeast B - type subunit, ProteinRts1p .

(321)

Thus, methylation of Pph21p is important for formation of PP2A trimeric and dimeric complexes, and consequently, for PP2A function.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	Thus, Methylationmethylation of ProteinPph21p is important for formation of PP2A trimeric and dimeric complexes, and consequently, for PP2A function.

(322)

Taken together, our results indicate that methylation and phosphorylation may be mechanisms by which the cell dynamically regulates PP2A complex formation and function.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(323)

Safety and pharmacokinetic study with escalating doses of 3 - acetyl - 7 - oxo - dehydroepiandrosterone in healthy male volunteers.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(324)

OBJECTIVES : To evaluate the safety and pharmacokinetics of 3 - acetyl - 7 - oxo - DHEA ( 3beta - acetoxyandrost - 5 - ene - 7, 17 - dione ) given orally.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(325)

DESIGN : A randomized, double blind, placebo - controlled, escalating dose study.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(326)

SETTING : The Chicago Center for Clinical Research.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(327)

PARTICIPANTS : Twenty - two healthy men.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(328)

STUDY METHOD : The participants received placebo ( n = 6 ) or 3 - acetyl - 7 - oxo - DHEA ( n = 16 ) at 50 mg / d for 7 days followed by a 7 - day washout ; 100 mg / d for 7 days followed by a 7 - day washout ; and 200 mg / d for 28 days.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(329)

OUTCOME MEASURES : Safety parameters, evaluated at each dose level, included measurement of total testosterone, free testosterone, dihydrotestosterone, estradiol, cortisol, thyroxin and insulin levels.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(330)

Analyses for 7 - oxo - DHEA - 3beta - sulfate ( DHEA - S ), the only detectable metabolic product of the administered steroid, were conducted on plasma drawn from all subjects at 0. 25, 0. 5, 1, 2, 4, 6 and 12 hours after the final 100 mg dose of 3beta - acetyl - 7 - oxo - DHEA.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(331)

RESULTS : There were no differences in the clinical laboratory values or in reported minor adverse experiences, between treatment and placebo groups.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(332)

In general, blood hormone concentrations were unaffected by the treatment with 3beta - acetyl - 7 - oxo - DHEA and remained within the normal range.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(333)

No changes in vital signs, blood chemistry or urinalysis occurred during treatment with 3beta - acetyl - 7 - oxo - DHEA compared to placebo.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(334)

The administered steroid was not detected in the blood but was rapidly converted to 7 - oxo - DHEA - S, the concentrations of which were proportional to dose.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(335)

This steroid sulfate did not accumulate ; plasma concentrations 12 hours after the 3beta - acetyl - 7 - oxo - DHEA dose at 7 and 28 days on the 200 mg / d dose were 15. 8 and 16. 3 microg / L respectively.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(336)

The mean time to peak plasma level of 7 - oxo - DHEA - S was 2. 2 hours ; the mean half life was 2. 17 hours.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(337)

The apparent clearance averaged 172 L / h, and the apparent mean volume of distribution was 540 L.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(338)

CONCLUSION : These results indicate that 3beta - acetyl - 7 - oxo - DHEA is safe and well tolerated in normal healthy men at doses up to 200 mg / d for 4 weeks.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(339)

Aureusidin synthase : a polyphenol oxidase homolog responsible for flower coloration.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(340)

Aurones are plant flavonoids that provide yellow color to the flowers of some popular ornamental plants, such as snapdragon and cosmos.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(341)

In this study, we have identified an enzyme responsible for the synthesis of aurone from chalcones in the yellow snapdragon flower.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(342)

The enzyme ( aureusidin synthase ) is a 39 - kilodalton, copper - containing glycoprotein catalyzing the hydroxylation and / or oxidative cyclization of the precursor chalcones, 2 ', 4 ', 6 ', 4 - tetrahydroxychalcone and 2 ', 4 ', 6 ', 3, 4 - pentahydroxychalcone.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(343)

The complementary DNA encoding aureusidin synthase is expressed in the petals of aurone - containing varieties.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(344)

DNA sequence analysis revealed that aureusidin synthase belongs to the plant polyphenol oxidase family, providing an unequivocal example of the function of the polyphenol oxidase homolog in plants, i. e., flower coloration.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(345)

E - cadherin expression is silenced by 5'CpG island methylation in acute leukemia.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Relation	Text (Head - Tail)
0.9991	Methylation	E - cadherin expression is silenced by Entity5'CpG island DNA_methylationmethylation in acute leukemia.
0.9994	Methylation	E - cadherin expression is silenced by 5 ' EntityCpG island DNA_methylationmethylation in acute leukemia.

(346)

E - Cadherin is a transmembrane glycoprotein that mediates Ca2 + - dependent intercellular adhesion in normal epithelium.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(347)

In tumors of epithelial origin, E - cadherin expression frequently is reduced, an event that contributes to tumor invasion and metastasis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(348)

The role of E - cadherin in hematopoietic tissues is less clear.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(349)

In normal bone marrow, E - cadherin is expressed on erythroid progenitors, CD34 + stem cells, and stromal cells, where it likely contributes to intercellular interactions during hematopoiesis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(350)

In this study, we used a nested - PCR approach to examine the methylation status of the E - cadherin 5'CpG island in blood and bone marrow samples from normal donors and in bone marrow from patients with acute leukemia.

Precision: 25.00

Recall: 100.00

F1: 40.00

Score	Relation	Text (Head - Tail)
0.9982	Methylation	In this study, we used a nested - PCR approach to examine the DNA_methylationmethylation status of the E - cadherin Entity5'CpG island in blood and bone marrow samples from normal donors and in bone marrow from patients with acute leukemia.
0.9988	Methylation	In this study, we used a nested - PCR approach to examine the DNA_methylationmethylation status of the E - cadherin 5 ' EntityCpG island in blood and bone marrow samples from normal donors and in bone marrow from patients with acute leukemia.
0.9984	Methylation	In this study, we used a nested - PCR approach to examine the DNA_methylationmethylation status of the E - cadherin 5 ' EntityCpG island in blood and bone marrow samples from normal donors and in bone marrow from patients with acute leukemia.
0.9968	Methylation	In this study, we used a nested - PCR approach to examine the DNA_methylationmethylation status of the E - cadherin Entity5'CpG island in blood and bone marrow samples from normal donors and in bone marrow from patients with acute leukemia.

(351)

In normal peripheral blood mononuclear cells and bone marrow, E - cadherin was completely unmethylated.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	In normal peripheral blood mononuclear cells and bone marrow, ProteinE - cadherin was completely DNA_methylationunmethylated .

(352)

In peripheral blood mononuclear cells, expression was evident by reverse transcription - PCR.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(353)

Immunoblotting confirmed E - cadherin protein expression in two lymphoblastoid cell lines derived from normal donors.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(354)

In contrast, E - cadherin was aberrantly methylated in 4 of 4 ( 100 % ) leukemia cell lines, 14 of 44 ( 32 % ) acute myelogenous leukemias, and 18 of 33 ( 53 % ) acute lymphoblastic leukemias.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	In contrast, ProteinE - cadherin was aberrantly DNA_methylationmethylated in 4 of 4 ( 100 % ) leukemia cell lines, 14 of 44 ( 32 % ) acute myelogenous leukemias, and 18 of 33 ( 53 % ) acute lymphoblastic leukemias.

(355)

Genomic bisulfite sequencing of primary leukemias confirmed dense methylation across the CpG island.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	Genomic bisulfite sequencing of primary leukemias confirmed dense DNA_methylationmethylation across the EntityCpG island .

(356)

Methylation was associated with loss of E - cadherin RNA and protein in leukemia cell lines and primary leukemias.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	DNA_methylationMethylation was associated with loss of ProteinE - cadherin RNA and protein in leukemia cell lines and primary leukemias.

(357)

Following treatment with 5 - aza - 2'- deoxycytidine, a methylated leukemia cell line expressed both E - cadherin transcript and protein.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9997	Methylation	Following treatment with 5 - aza - 2'- deoxycytidine, a DNA_methylationmethylated leukemia cell line expressed both ProteinE - cadherin transcript and protein.

(358)

Our results show that methylation of E - cadherin occurs commonly in acute leukemia and suggests a hypothesis for E - cadherin down - regulation in leukemogenesis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9998	Methylation	Our results show that DNA_methylationmethylation of ProteinE - cadherin occurs commonly in acute leukemia and suggests a hypothesis for E - cadherin down - regulation in leukemogenesis.

(359)

Defect in N - glycosylation of proteins is tissue - dependent in congenital disorders of glycosylation Ia.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(360)

The biochemical hallmark of Congenital Disorders of Glycosylation ( CDG ) including type Ia is a defective N - glycosylation of serum glycoproteins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(361)

Hypoglycosylated forms of alpha1 - antitrypsin have been detected by Western blot in serum from CDG Ia patients.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	GlycosylationHypoglycosylated forms of Proteinalpha1 - antitrypsin have been detected by Western blot in serum from CDG Ia patients.

(362)

In contrast we were not able to detect hypoglycosylation in alpha1 - antitrypsin synthesized by fibroblasts, keratinocytes, enterocytes, and leukocytes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	In contrast we were not able to detect Glycosylationhypoglycosylation in Proteinalpha1 - antitrypsin synthesized by fibroblasts, keratinocytes, enterocytes, and leukocytes.

(363)

Similarly no hypoglycosylation was detectable in a membrane - associated N - linked glycoprotein, the facilitative glucose transporter GLUT - 1 and also in serum immunoglobulin G isolated from sera of CDG Ia patients.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9995	Methylation	Similarly no Glycosylationhypoglycosylation was detectable in a membrane - associated N - linked glycoprotein, the facilitative glucose transporter ProteinGLUT - 1 and also in serum immunoglobulin G isolated from sera of CDG Ia patients.

(364)

We conclude that the phenotypic expression of CDG Ia is tissue - dependent.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(365)

Molecular cloning and characterization of CHM1L, a novel membrane molecule similar to chondromodulin - I.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(366)

Chondromodulin - I ( ChM - I ) is a cartilage - specific glycoprotein that stimulates the growth of chondrocytes and inhibits the tube formation of endothelial cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(367)

In the present study, we identified a novel ChM - I like molecule, designated ChM1L.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(368)

Cloning of full length cDNAs of human, mouse, and rat ChM1L revealed that ChM1L encodes 317 amino acids novel type II transmembrane protein.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(369)

ChM1L protein was expressed on the cell surface as N - glycosylated and non - N - glycosylated protein with molecular mass of 45 and 40 kDa, respectively.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	ProteinChM1L protein was expressed on the cell surface as GlycosylationN - glycosylated and non - N - glycosylated protein with molecular mass of 45 and 40 kDa, respectively.
0.9987	Methylation	ProteinChM1L protein was expressed on the cell surface as N - glycosylated and Glycosylationnon - N - glycosylated protein with molecular mass of 45 and 40 kDa, respectively.

(370)

In adult mouse tissues, ChM1L mRNA was highly expressed in eye, skeletal muscle, and whole rib.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(371)

The temporal pattern of ChM1L mRNA was examined using whole embryo at day 10 to 19 of gestation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(372)

After day 11, ChM1L mRNA was detected and its level was progressively elevated in association with development of mouse embryo.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(373)

These data suggest that ChM1L is a novel membrane molecule which is similar to ChM - I that plays a regulatory role in eye, skeletal muscle, and development of embryo.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(374)

Targeting of calsequestrin to the sarcoplasmic reticulum of skeletal muscle upon deletion of its glycosylation site.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(375)

The glycoprotein calsequestrin ( CS ) is segregated to the junctional sarcoplasmic reticulum ( jSR ) and is responsible for intraluminal Ca ( 2 + ) binding.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(376)

A chimeric CS - hemoagglutinin 1 ( HA1 ), obtained by adding the nine amino acid viral epitope hemoagglutinin to the carboxy terminal of CS and shown to be correctly segregated to skeletal muscle jSR [ A.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(377)

Nori, K.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(378)

A.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(379)

Nadalini, A.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(380)

Martini, R.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(381)

Rizzuto, A.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(382)

Villa, and P.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(383)

Volpe ( 1997 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(384)

Chimeric calsequestrin and its targeting to the junctional sarcoplasmic reticulum of skeletal muscle.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(385)

Am.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(386)

J.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(387)

Physiol. 272, C1420 - C1428 ] lends itself as a molecular tool to investigate the targeting domains of CS.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(388)

A putative targeting mechanism of CS to jSR implies glycosylation - dependent steps in the endoplasmic reticulum ( ER ) and Golgi complex.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(389)

To test this hypothesis, CS - HA1DeltaGly, a mutant in which the unique N - glycosylation site Asn316 was changed to Ile, was engineered by site - directed mutagenesis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(390)

The mutant cDNA was transiently transfected in either HeLa cells, myoblasts of rat skeletal muscle primary cultures, or regenerating soleus muscle fibers of adult rats.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(391)

The expression and intracellular localization of CS - HA1DeltaGly was studied by double - labeling epifluorescence by means of antibodies against either CS, HA1, or the ryanodine receptor calcium release channel.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(392)

CS - HA1DeltaGly was expressed and retained to ER and ER / sarcoplasmic reticulum of HeLa cells and myotubes, respectively, and expressed, sorted, and correctly segregated to jSR of regenerating soleus muscle fibers.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(393)

Thus, the targeting mechanism of CS in vivo appears not to be affected by glycosylation - that is, the sorting, docking, and segregation of CS are independent of cotranslational and posttranslational glycosylation or glycosylations.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(394)

Targeting of HIF - alpha to the von Hippel - Lindau ubiquitylation complex by O2 - regulated prolyl hydroxylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(395)

Hypoxia - inducible factor ( HIF ) is a transcriptional complex that plays a central role in the regulation of gene expression by oxygen.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(396)

In oxygenated and iron replete cells, HIF - alpha subunits are rapidly destroyed by a mechanism that involves ubiquitylation by the von Hippel - Lindau tumor suppressor ( pVHL ) E3 ligase complex.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Relation	Text (Head - Tail)
0.8783	Methylation	In oxygenated and iron replete cells, HIF - alpha subunits are rapidly destroyed by a mechanism that involves Ubiquitinationubiquitylation by the von ProteinHippel - Lindau tumor suppressor ( pVHL ) E3 ligase complex.
0.6192	Methylation	In oxygenated and iron replete cells, HIF - alpha subunits are rapidly destroyed by a mechanism that involves Ubiquitinationubiquitylation by the Proteinvon Hippel - Lindau tumor suppressor ( pVHL ) E3 ligase complex.

(397)

This process is suppressed by hypoxia and iron chelation, allowing transcriptional activation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(398)

Here we show that the interaction between human pVHL and a specific domain of the HIF - 1alpha subunit is regulated through hydroxylation of a proline residue ( HIF - 1alpha P564 ) by an enzyme we have termed HIF - alpha prolyl - hydroxylase ( HIF - PH ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9980	Methylation	Here we show that the interaction between human pVHL and a specific domain of the HIF - 1alpha subunit is regulated through Hydroxylationhydroxylation of a proline residue ( HIF - 1alpha EntityP564 ) by an enzyme we have termed HIF - alpha prolyl - hydroxylase ( HIF - PH ).

(399)

An absolute requirement for dioxygen as a cosubstrate and iron as cofactor suggests that HIF - PH functions directly as a cellular oxygen sensor.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(400)

HIFalpha targeted for VHL - mediated destruction by proline hydroxylation : implications for O2 sensing.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(401)

HIF ( hypoxia - inducible factor ) is a transcription factor that plays a pivotal role in cellular adaptation to changes in oxygen availability.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(402)

In the presence of oxygen, HIF is targeted for destruction by an E3 ubiquitin ligase containing the von Hippel - Lindau tumor suppressor protein ( pVHL ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(403)

We found that human pVHL binds to a short HIF - derived peptide when a conserved proline residue at the core of this peptide is hydroxylated.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(404)

Because proline hydroxylation requires molecular oxygen and Fe ( 2 + ), this protein modification may play a key role in mammalian oxygen sensing.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(405)

Genetic and clinical features of human pancreatic ductal adenocarcinomas with widespread microsatellite instability.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(406)

The incidences of microsatellite instability ( MSI ) and underlying DNA mismatch repair ( MMR ) defects in pancreatic carcinogenesis have not been well established.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(407)

We analyzed 100 sporadic and 3 hereditary pancreatic ductal adenocarcinomas for MSI, and high - frequency MSI ( MSI - H ) and low - frequency MSI ( MSI - L ) tumors were further analyzed for frameshift mutations of possible target genes and for promoter methylation and mutation of DNA MMR genes, including hMLH1, hMSH2, hMSH3, and hMSH6 genes.

Precision: 33.33

Recall: 100.00

F1: 50.00

Score	Relation	Text (Head - Tail)
0.9979	Methylation	We analyzed 100 sporadic and 3 hereditary pancreatic ductal adenocarcinomas for MSI, and high - frequency MSI ( MSI - H ) and low - frequency MSI ( MSI - L ) tumors were further analyzed for frameshift mutations of possible target genes and for Entitypromoter DNA_methylationmethylation and mutation of DNA MMR genes, including hMLH1, hMSH2, hMSH3, and hMSH6 genes.
0.9610	Methylation	We analyzed 100 sporadic and 3 hereditary pancreatic ductal adenocarcinomas for MSI, and high - frequency MSI ( MSI - H ) and low - frequency MSI ( MSI - L ) tumors were further analyzed for frameshift mutations of possible target genes and for Entitypromoter DNA_methylationpromoter methylation and mutation of DNA MMR genes, including hMLH1, hMSH2, hMSH3, and hMSH6 genes.
0.8969	Methylation	We analyzed 100 sporadic and 3 hereditary pancreatic ductal adenocarcinomas for MSI, and high - frequency MSI ( MSI - H ) and low - frequency MSI ( MSI - L ) tumors were further analyzed for frameshift mutations of possible target genes and for DNA_methylationpromoter methylation DNA_methylationmethylation and mutation of DNA MMR genes, including hMLH1, hMSH2, hMSH3, and hMSH6 genes.

(408)

Among the 100 sporadic tumors, 13 ( 13 % ) were MSI - H, 13 ( 13 % ) were MSI - L, and 74 ( 74 % ) were microsatellite stable ( MSS ) tumors.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(409)

All of the three hereditary tumors from hereditary nonpolyposis colorectal cancer ( HNPCC ) patients were MSI - H.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(410)

MSI - H tumors were significantly associated with poor differentiation and the presence of wild - type K - RAS and p53 genes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(411)

Patients with MSI - H tumors had a significantly longer overall survival time than did those with MSI - L or MSS tumors ( P = 0. 0057 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(412)

Frameshift mutations of hMSH3, hMLH3, BRCA - 2, TGF - beta type II receptor, and BAX genes were detected in MSI - H tumors.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(413)

Hypermethylation of the hMLH1 promoter was observed in 6 ( 46 % ) of the 13 sporadic MSI - H tumors but not in any of the 3 hereditary MSI - H tumors or 13 MSI - L tumors.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9977	Methylation	DNA_methylationHypermethylation of the hMLH1 Entitypromoter was observed in 6 ( 46 % ) of the 13 sporadic MSI - H tumors but not in any of the 3 hereditary MSI - H tumors or 13 MSI - L tumors.

(414)

All of the 3 HNPCC cases had germ - line hMLH1 mutation accompanied by loss of heterogeneity or other mutation in the tumor.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(415)

Our results suggest that pancreatic carcinomas with MSI - H represent a distinctive oncogenic pathway because they exhibit peculiar clinical, pathological, and molecular characteristics.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(416)

Our results also suggest the principal involvement of epigenetic or genetic inactivation of the hMLH1 gene in the pathogenesis of pancreatic carcinoma with MSI - H.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(417)

The acetylation state of human fetal hemoglobin modulates the strength of its subunit interactions : long - range effects and implications for histone interactions in the nucleosome.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(418)

The source of the 70 - fold increased tetramer strength of liganded fetal hemoglobin relative to that of adult hemoglobin between pH 6. 0 and 7. 5 reported earlier [ Dumoulin et al. ( 1997 ) J.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(419)

Biol.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(420)

Chem. 272, 31326 ] has been identified as the N - terminal Gly residue of the gamma - chain, which is replaced by Val in adult hemoglobin.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(421)

This was revealed by extending the study of the pH dependence of the tetramer - dimer equilibrium of these hemoglobins into the alkaline range as far as pH 9.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(422)

From pH 7. 5 to 9. 0, the 70 - fold difference in the association equilibrium constant between hemoglobins F and A lessened progressively.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(423)

This behavior was attributed to the difference in the pK ( a ) 8. 1 of Gly - 1 ( gamma ) compared to the pK ( a ) 7. 1 value of Val - 1 ( beta ) of hemoglobins F and A, respectively.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(424)

Evidence for this conclusion was obtained by demonstrating that natural hemoglobin F ( 1 ), which is specifically acetylated at Gly - 1 ( gamma ) and hence unable to be protonated, behaves like HbA and not HbF in its tetramer - dimer association properties over the pH range studied.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(425)

An increased degree of protonation of the gamma - chain N - terminus of hemoglobin F from pH 9. 0 to 8. 0 is therefore suggested as responsible for its increased tetramer strength representing an example of transmission of a signal from its positively charged N - terminal tail to the distant subunit allosteric interface where the equilibrium constant is measured.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(426)

An analogy is made between the effects of acetylation of the fetal hemoglobin tetramer on the strength of its subunit interactions and acetylation of some internal Lys residues within the N - terminal segments of the histone octamer around which DNA is wrapped in the nucleosome.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(427)

Synergism of Xist RNA, DNA methylation, and histone hypoacetylation in maintaining X chromosome inactivation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9997	Methylation	Synergism of Xist RNA, DNA methylation, and Proteinhistone Acetylationhypoacetylation in maintaining X chromosome inactivation.

(428)

Xist RNA expression, methylation of CpG islands, and hypoacetylation of histone H4 are distinguishing features of inactive X chromatin.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9998	Methylation	Xist RNA expression, methylation of CpG islands, and Acetylationhypoacetylation of Proteinhistone H4 are distinguishing features of inactive X chromatin.

(429)

Here, we show that these silencing mechanisms act synergistically to maintain the inactive state.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(430)

Xist RNA has been shown to be essential for initiation of X inactivation, but not required for maintenance.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(431)

We have developed a system in which the reactivation frequency of individual X - linked genes can be assessed quantitatively.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(432)

Using a conditional mutant Xist allele, we provide direct evidence for that loss of Xist RNA destabilizes the inactive state in somatic cells, leading to an increased reactivation frequency of an X - linked GFP transgene and of the endogenous hypoxanthine phosphoribosyl transferase ( Hprt ) gene in mouse embryonic fibroblasts.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(433)

Demethylation of DNA, using 5 - azadC or by introducing a mutation in Dnmt1, and inhibition of histone hypoacetylation using trichostatin A further increases reactivation in Xist mutant fibroblasts, indicating a synergistic interaction of X chromosome silencing mechanisms.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9997	Methylation	Demethylation of DNA, using 5 - azadC or by introducing a mutation in Dnmt1, and inhibition of Proteinhistone Acetylationhypoacetylation using trichostatin A further increases reactivation in Xist mutant fibroblasts, indicating a synergistic interaction of X chromosome silencing mechanisms.

(434)

Expression of Pichia anomala INV1 gene in Saccharomyces cerevisiae results in two different active forms of hypoglycosylated invertase.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Relation	Text (Head - Tail)
0.9972	Methylation	Expression of Pichia anomala ProteinINV1 gene in Saccharomyces cerevisiae results in two different active forms of Glycosylationhypoglycosylated invertase.
0.9017	Methylation	Expression of Pichia anomala ProteinINV1 gene in Saccharomyces cerevisiae results in two different active forms of Glycosylationhypoglycosylated invertase .

(435)

The Pichia anomala invertase gene ( INV1 ) was introduced at different copy numbers into a sucrose - nonfermenting mutant of Saccharomyces cerevisiae and expressed from its own promoter sequences.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(436)

The level reached in the production of invertase by the transformants ( up to 540 units / 10 ( 10 ) cells ) was in agreement with the INV1 gene dosage.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(437)

Two forms of multimeric active and glycosylated invertase displaying different subcellular locations and molecular masses could be detected in the transformants.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9994	Methylation	Two forms of multimeric active and Glycosylationglycosylated Proteininvertase displaying different subcellular locations and molecular masses could be detected in the transformants.

(438)

One was found to be present in the culture medium and in the periplasm, and the other could only be detected inside the cell.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(439)

Each of the two heterologous forms of invertase was shown to be an oligomer composed of identical subunits.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(440)

The difference found in the apparent molecular masses of their monomers ( 81. 5 and 78. 3 kDa, respectively ) seems to be due to the size of their N - linked oligosaccharide chains ( on average 2. 4 and 1. 9 kDa, respectively ), since the number of sugar chains ( 9 ) and the molecular mass of the protein moiety ( 60. 5 kDa ) are identical in both forms.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(441)

The shorter size of their oligosaccharides must also be the reason for the lower apparent molecular masses of the heterologous invertases when compared with the enzyme purified from P. anomala.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(442)

The hypoglycosylated invertase accumulated within the cells of the transformants to an unusual level ( up to 130 units / 10 ( 10 ) cells ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9988	Methylation	The Glycosylationhypoglycosylated Proteininvertase accumulated within the cells of the transformants to an unusual level ( up to 130 units / 10 ( 10 ) cells ).

(443)

Such accumulation of active enzyme inside the cells, as well as its underglycosylation, could be due to intrinsic properties of the P. anomala invertase that are determined by the particular primary structure of its protein moiety.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	Such accumulation of active enzyme inside the cells, as well as its Glycosylationunderglycosylation , could be due to intrinsic properties of the P. anomala Proteininvertase that are determined by the particular primary structure of its protein moiety.

(444)

The histone acetyltransferase, hGCN5, interacts with and acetylates the HIV transactivator, Tat.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	The histone acetyltransferase, hGCN5, interacts with and Acetylationacetylates the HIV transactivator, ProteinTat .
0.9996	Methylation	The histone acetyltransferase, ProteinhGCN5 , interacts with and Acetylationacetylates the HIV transactivator, Tat.

(445)

Factor acetyltransferase activity associated with several histone acetyltransferases plays a key role in the control of transcription.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(446)

Here we report that hGCN5, a well known histone acetyltransferase, specifically interacts with and acetylates the human immunodeficiency virus type 1 ( HIV - 1 ) transactivator protein, Tat.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9998	Methylation	Here we report that hGCN5, a well known histone acetyltransferase, specifically interacts with and Acetylationacetylates the human immunodeficiency virus type 1 ( HIV - 1 ) transactivator protein, ProteinTat .
0.9976	Methylation	Here we report that ProteinhGCN5 , a well known histone acetyltransferase, specifically interacts with and Acetylationacetylates the human immunodeficiency virus type 1 ( HIV - 1 ) transactivator protein, Tat.

(447)

The interaction between Tat and hGCN5 is direct and involves the acetyltransferase and the bromodomain regions of hGCN5, as well as a limited region of Tat encompassing the cysteine - rich domain of the protein.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(448)

Tat lysines 50 and 51, target of acetylation by p300 / CBP, were also found to be acetylated by hGCN5.

Precision: 55.56

Recall: 100.00

F1: 71.43

Score	Relation	Text (Head - Tail)
1.0000	Methylation	Tat lysines 50 and Entity51 , target of Acetylationacetylation by p300 / CBP, were also found to be acetylated by hGCN5.
0.9998	Methylation	Tat lysines 50 and 51, target of acetylation by p300 / CBP, were also found to be Acetylationacetylated by ProteinhGCN5 .
0.9980	Methylation	Tat lysines 50 and Entity51 , target of acetylation by p300 / CBP, were also found to be Acetylationacetylated by hGCN5.
0.9974	Methylation	Tat Entitylysines 50 and 51, target of Acetylationacetylation by p300 / CBP, were also found to be acetylated by hGCN5.
0.9837	Methylation	Tat Entitylysines 50 and 51, target of acetylation by p300 / CBP, were also found to be Acetylationacetylated by hGCN5.
0.9991	Methylation	Tat lysines Entity50 and 51, target of Acetylationacetylation by p300 / CBP, were also found to be acetylated by hGCN5.
0.9942	Methylation	Tat lysines Entity50 and 51, target of acetylation by p300 / CBP, were also found to be Acetylationacetylated by hGCN5.
0.9893	Methylation	Tat Entitylysines 50 and 51, target of Acetylationacetylation by p300 / CBP, were also found to be acetylated by hGCN5.
0.9396	Methylation	Tat Entitylysines 50 and 51, target of acetylation by p300 / CBP, were also found to be Acetylationacetylated by hGCN5.

(449)

The acetylation of these two lysines by p300 / CBP has been recently shown to stimulate Tat transcriptional activity and accordingly, we have found that hGCN5 can considerably enhance Tat - dependent transcription of the HIV - 1 long terminal repeat.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9998	Methylation	The Acetylationacetylation of these two Entitylysines by p300 / CBP has been recently shown to stimulate Tat transcriptional activity and accordingly, we have found that hGCN5 can considerably enhance Tat - dependent transcription of the HIV - 1 long terminal repeat.

(450)

These data highlight the importance of the acetylation of lysines 50 and 51 in the function of Tat, since different histone acetyltransferases involved in distinct signaling pathways, GCN5 and p300 / CBP, converge to acetylate Tat on the same site.

Precision: 71.43

Recall: 100.00

F1: 83.33

Score	Relation	Text (Head - Tail)
1.0000	Methylation	These data highlight the importance of the Acetylationacetylation of Entitylysines 50 and 51 in the function of Tat, since different histone acetyltransferases involved in distinct signaling pathways, GCN5 and p300 / CBP, converge to acetylate Tat on the same site.
0.9989	Methylation	These data highlight the importance of the acetylation of lysines 50 and 51 in the function of Tat, since different histone acetyltransferases involved in distinct signaling pathways, ProteinGCN5 and p300 / CBP, converge to Acetylationacetylate Tat on the same site.
0.9986	Methylation	These data highlight the importance of the acetylation of lysines 50 and Entity51 in the function of Tat, since different histone acetyltransferases involved in distinct signaling pathways, GCN5 and p300 / CBP, converge to Acetylationacetylate Tat on the same site.
0.9957	Methylation	These data highlight the importance of the Acetylationacetylation of lysines 50 and Entity51 in the function of Tat, since different histone acetyltransferases involved in distinct signaling pathways, GCN5 and p300 / CBP, converge to acetylate Tat on the same site.
0.9925	Methylation	These data highlight the importance of the acetylation of Entitylysines 50 and 51 in the function of Tat, since different histone acetyltransferases involved in distinct signaling pathways, GCN5 and p300 / CBP, converge to Acetylationacetylate Tat on the same site.
0.9998	Methylation	These data highlight the importance of the Acetylationacetylation of Entitylysines 50 and 51 in the function of Tat, since different histone acetyltransferases involved in distinct signaling pathways, GCN5 and p300 / CBP, converge to acetylate Tat on the same site.
0.9657	Methylation	These data highlight the importance of the acetylation of Entitylysines 50 and 51 in the function of Tat, since different histone acetyltransferases involved in distinct signaling pathways, GCN5 and p300 / CBP, converge to Acetylationacetylate Tat on the same site.

(451)

Hydroxylating activity of frog epidermis tyrosinase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(452)

Trypsin activated in a similar way both the tyrosine hydroxylase and the dopa - oxidasa activities of frog epidermis tyrosinase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(453)

Several electron donors reduced or eliminated the lag period for the hydroxylating enzyme. 4 x 10 ( - 5 ) M dopa was particularly effective, but without affecting the stationary activity after lag period.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(454)

Tyrosine hydroxylase had KM = 2. 6 X 10 ( - 3 ) M for tyrosine and 2 x 10 ( - 3 ) M dopa was a competitive inhibitor with Ki = 5 x 10 ( - 4 ) M.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(455)

The enzyme was inactivated during its actuation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(456)

Data on thermal denaturation were similar to other obtained from dopa oxidase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(457)

Our results tend to confirm our previous hypothesis that the activatory process of the enzyme is accompanied by a spatial unfolding of the enzyme molecule.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(458)

Canine COL1A2 mutation resulting in C - terminal truncation of pro - alpha2 ( I ) and severe osteogenesis imperfecta.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(459)

RNA and type I collagen were analyzed from cultured skin fibroblasts of a Beagle puppy with fractures consistent with type III osteogenesis imperfecta ( OI ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(460)

In a nonisotopic RNAse cleavage assay ( NIRCA ), the proband's RNA had a unique cleavage pattern in the region of COL1A2 encoding the C - propeptide.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(461)

DNA sequence analyses identified a mutation in which nucleotides 3991 - 3994 ( " CTAG " ) were replaced with " TGTCATTGG. " The first seven bases of the inserted sequence were identical to nucleotides 4002 - 4008 of the normal canine COL1A2 sequence.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(462)

The resulting frameshift changed 30 amino acids and introduced a premature stop codon.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(463)

Reverse - transcription polymerase chain reaction ( RT - PCR ) with primers flanking the mutation site amplified two complementary DNA ( cDNA ) fragments for the proband and a single product for the control.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(464)

Restriction enzyme digestions also were consistent with a heterozygous mutation in the proband.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(465)

Type I procollagen labeled with [ 3H ] proline was analyzed by sodium dodecyl sulfate - polyacrylamide gel electrophoresis ( SDS - PAGE ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(466)

Increased density of pC - alpha2 ( I ) suggested comigration with the similarly sized pro - alpha2 ( I ) derived from the mutant allele.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(467)

Furthermore, a - chains were overhydroxylated and the ratio of alpha1 ( I ) : alpha2 ( I ) was 3. 2 : 1, consistent with the presence of alpha1 ( I ) homotrimers.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9997	Methylation	Furthermore, a - chains were Hydroxylationoverhydroxylated and the ratio of Proteinalpha1 ( I ) : alpha2 ( I ) was 3. 2 : 1, consistent with the presence of alpha1 ( I ) homotrimers.

(468)

Analyses of COL1A2 and type I collagen were both consistent with the described heterozygous mutation affecting the pro - alpha2 ( I ) C - propeptide and confirmed a diagnosis of OI.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(469)

Linking global histone acetylation to the transcription enhancement of X - chromosomal genes in Drosophila males.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9991	Methylation	Linking global Proteinhistone Acetylationacetylation to the transcription enhancement of X - chromosomal genes in Drosophila males.

(470)

It has become well established for several genes that targeting of histone acetylation to promoters is required for the activation of transcription.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9997	Methylation	It has become well established for several genes that targeting of Proteinhistone Acetylationacetylation to promoters is required for the activation of transcription.

(471)

In contrast, global patterns of acetylation have not been ascribed to any particular regulatory function.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(472)

In Drosophila, a specific modification of H4, acetylation at lysine 16, is enriched at hundreds of sites on the male X chromosome due to the activity of the male - specific lethal ( MSL ) dosage compensation complex.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Relation	Text (Head - Tail)
0.9997	Methylation	In Drosophila, a specific modification of H4, Acetylationacetylation at Entitylysine 16 , is enriched at hundreds of sites on the male X chromosome due to the activity of the male - specific lethal ( MSL ) dosage compensation complex.
0.9985	Methylation	In Drosophila, a specific modification of H4, Acetylationacetylation at Entitylysine 16, is enriched at hundreds of sites on the male X chromosome due to the activity of the male - specific lethal ( MSL ) dosage compensation complex.

(473)

Utilizing chromatin immunoprecipitation, we have determined that H4Ac16 is present along the entire length of X - linked genes targeted by the MSL complex with relatively modest levels of acetylation at the promoter regions and high levels in the middle and / or 3'end of the transcription units.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9997	Methylation	Utilizing chromatin immunoprecipitation, we have determined that ProteinH4Ac16 is present along the entire length of X - linked genes targeted by the MSL complex with relatively modest levels of Acetylationacetylation at the promoter regions and high levels in the middle and / or 3'end of the transcription units.

(474)

We propose that global acetylation by the MSL complex increases the expression of X - linked genes by facilitating transcription elongation rather than by enhancing promoter accessibility.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(475)

We have also determined that H4Ac16 is absent from a region of the X chromosome that includes a gene known to be dosage - compensated by a MSL - independent mechanism.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(476)

This study represents the first biochemical interpretation of the very large body of cytological observations on the chromosomal distribution of the MSL complex.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(477)

Correlation between histone lysine methylation and developmental changes at the chicken beta - globin locus.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(478)

Methylation of histones at specific residues plays an important role in transcriptional regulation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9996	Methylation	MethylationMethylation of Proteinhistones at specific residues plays an important role in transcriptional regulation.

(479)

Chromatin immunoprecipitation of dimethylated lysine 9 on histone H3 across 53 kilobases of the chicken beta - globin locus during erythropoiesis shows an almost complete anticorrelation between regions of elevated lysine 9 methylation and acetylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9308	Methylation	Chromatin immunoprecipitation of dimethylated lysine 9 on histone H3 across 53 kilobases of the chicken beta - globin locus during erythropoiesis shows an almost complete anticorrelation between regions of elevated Entitylysine 9 Methylationmethylation and acetylation.
0.9222	Methylation	Chromatin immunoprecipitation of dimethylated lysine 9 on Proteinhistone H3 across 53 kilobases of the chicken beta - globin locus during erythropoiesis shows an almost complete anticorrelation between regions of elevated lysine 9 methylation and Acetylationacetylation .

(480)

Lysine 9 is methylated most over constitutive condensed chromatin and developmentally inactive globin genes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(481)

In contrast, lysine 4 methylation of histone H3 correlates with H3 acetylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(482)

These results lead us to propose a mechanism by which the insulator in the beta - globin locus can protect the globin genes from being silenced by adjacent condensed chromatin.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(483)

Purification and characterization of a soluble bioactive amino - terminal extracellular domain of the human thyrotropin receptor.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(484)

The amino - terminal ectodomain of the human TSH receptor has been expressed at the surface of CHO cells as a glycosylphosphatidylinositol - anchored molecule containing a 10 - residue histidine tag close to its C terminus.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Relation	Text (Head - Tail)
	Methylation	The amino - terminal ectodomain of the human TSH receptor has been expressed at the surface of CHO cells as a Entityglycosylphosphatidylinositol - anchored Entityglycosylphosphatidylinositol - anchored molecule containing a 10 - residue histidine tag close to its C terminus.

(485)

The soluble ectodomain could be released from the cells by treatment with a glycosylphosphatidylinositol - phospholipase C and purified to apparent homogeneity by cobalt - Sepharose chromatography.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(486)

Two nanomoles of material was obtained, which was suitable for analysis by mass spectrometry.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(487)

This allowed the identification of four out of the six potential N - glycosylation sites as being effectively glycosylated.

Precision: 33.33

Recall: 100.00

F1: 50.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	This allowed the identification of four out of the six potential EntityN - glycosylation sites as being effectively Glycosylationglycosylated .
0.9992	Methylation	This allowed the identification of four out of the six potential EntityN - glycosylation sites as being effectively Glycosylationglycosylated .
0.4461	Methylation	This allowed the identification of four out of the six potential EntityN - glycosylation sites EntityN - glycosylation sites as being effectively glycosylated.

(488)

A proportion of the purified soluble ectodomain displayed specific binding of ( 125 ) I - labeled TSH, allowing for the first time performance of classical saturation binding experiments.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(489)

Two classes of high - affinity binding sites were identified : site A, K ( d ) 0. 014 nM ; site B, K ( d ) 0. 83 nM.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(490)

The significance of site A, whose affinity is much higher than for the holoreceptor at the surface of intact cells, remains to be clarified.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(491)

The purified ectodomain was capable of inhibiting efficiently the thyroid stimulating activity of immunoglobulins from patients with Graves'disease.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(492)

It allowed computation of the amounts of these immunoglobulins in patient's serum, giving values up to 10 microg / mL.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(493)

Contrary to all currently available assays, the soluble ectodomain of the TSH receptor purified in a functionally competent conformation allows direct studies of its interactions with TSH and autoantibodies and opens the way to structural studies.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(494)

HIF - 1alpha binding to VHL is regulated by stimulus - sensitive proline hydroxylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9980	Methylation	HIF - 1alpha binding to VHL is regulated by stimulus - sensitive Entityproline Hydroxylationhydroxylation .

(495)

Hypoxia - inducible factor - 1alpha ( HIF - 1alpha ) is a global transcriptional regulator of the hypoxic response.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(496)

Under normoxic conditions, HIF - 1alpha is recognized by the von Hippel - Lindau tumor - suppressor protein ( VHL ), a component of an E3 ubiquitin ligase complex.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(497)

This interaction thereby promotes the rapid degradation of HIF - 1alpha.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(498)

Under hypoxic conditions, HIF - 1alpha is stabilized.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(499)

We have previously shown that VHL binds in a hypoxia - sensitive manner to a 27 - aa segment of HIF - 1alpha, and that this regulation depends on a posttranslational modification of HIF - 1alpha.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(500)

Through a combination of in vivo coimmunoprecipitation assays using VHL and a panel of point mutants of HIF - 1alpha in this region, as well as MS and in vitro binding assays, we now provide evidence that this modification, which occurs under normoxic conditions, is hydroxylation of Pro - 564 of HIF - 1alpha.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9997	Methylation	Through a combination of in vivo coimmunoprecipitation assays using VHL and a panel of point mutants of HIF - 1alpha in this region, as well as MS and in vitro binding assays, we now provide evidence that this modification, which occurs under normoxic conditions, is Hydroxylationhydroxylation of EntityPro - 564 of HIF - 1alpha.

(501)

The data furthermore show that this proline hydroxylation is the primary regulator of VHL binding.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(502)

2B4 ( CD244 ) and CS1 : novel members of the CD2 subset of the immunoglobulin superfamily molecules expressed on natural killer cells and other leukocytes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(503)

2B4 is a member of the CD2 subset of the immunoglobulin superfamily molecules expressed on natural killer ( NK ) cells and other leukocytes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(504)

It is the high affinity ligand for CD48.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(505)

Engagement of 2B4 on NK - cell surfaces with specific antibodies or CD48 can trigger cell - mediated cytotoxicity, interferon - gamma secretion, phosphoinositol turnover and NK - cell invasiveness.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(506)

The function of 2B4 in CD8 + T cells and myeloid cells remains unknown.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(507)

The cytoplasmic domain of 2B4 contains unique tyrosine motifs ( TxYxxV / I ) that associate with src homology 2 domain - containing protein or signaling lymphocyte activation molecule ( SLAM ) - associated protein, whose mutation is the underlying genetic defect in the X - linked lymphoproliferative disease ( XLPD ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(508)

Impaired signaling via 2B4 and SLAM is implicated in the immunopathogenesis of XLPD.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(509)

CS1 is a novel member of the CD2 subset that contains two of the unique tyrosine motifs present in 2B4 and SLAM.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(510)

Signaling through 2B4, CS1 and other members of the CD2 subset may play a major role in the regulation of NK cells and other leukocyte functions.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(511)

N - glycosylation of CRF receptor type 1 is important for its ligand - specific interaction.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	GlycosylationN - glycosylation of ProteinCRF receptor type 1 is important for its ligand - specific interaction.

(512)

The corticotropin - releasing factor ( CRF ) receptor type 1 ( CRFR1 ) contains five potential N - glycosylation sites : N38, N45, N78, N90, and N98.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(513)

Cells expressing CRFR1 were treated with tunicamycin to block receptor glycosylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(514)

The nonglycosylated receptor did not bind the radioligand and had a decreased cAMP stimulation potency in response to CRF.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(515)

To determine which of the polysaccharide chain ( s ) is / are involved in ligand interaction, the polysaccharide chains were deleted using site - directed mutagenesis of the glycosylation consensus, N - X - S / T.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(516)

Two sets of mutations were performed for each glycosylation site : N to Q and S / T to A, respectively.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(517)

The single mutants Q38, Q45, Q78, Q90, Q98, A40, A47, A80, A92, and A100 and the double mutants A40 / A47 and A80 / A100 were well expressed, bound CRF, sauvagine ( SVG ), and urotensin - I ( UTS - I ) with a normal affinity, and increased cAMP accumulation with a high efficiency.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(518)

In contrast, the combined mutations A80 / A92 / A100, A40 / A80 / A92 / A100, and A40 / A47 / A80 / A92 / A100 had low levels of expression, did not bind the radioligand, and had a decreased cAMP stimulation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(519)

These data indicate the requirement for three or more polysaccharide chains for normal CRFR1 function.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(520)

Reduced rates of gene loss, gene silencing, and gene mutation in Dnmt1 - deficient embryonic stem cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(521)

Tumor suppressor gene inactivation is a crucial event in oncogenesis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(522)

Gene inactivation mechanisms include events resulting in loss of heterozygosity ( LOH ), gene mutation, and transcriptional silencing.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(523)

The contribution of each of these different pathways varies among tumor suppressor genes and by cancer type.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(524)

The factors that influence the relative utilization of gene inactivation pathways are poorly understood.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(525)

In this study, we describe a detailed quantitative analysis of the three major gene inactivation mechanisms for a model gene at two different genomic integration sites in mouse embryonic stem ( ES ) cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(526)

In addition, we targeted the major DNA methyltransferase gene, Dnmt1, to investigate the relative contribution of DNA methylation to these various competing gene inactivation pathways.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(527)

Our data show that gene loss is the predominant mode of inactivation of a herpes simplex virus thymidine kinase neomycin phosphotransferase reporter gene ( HSV - TKNeo ) at the two integration sites tested and that this event is significantly reduced in Dnmt1 - deficient cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(528)

Gene silencing by promoter methylation requires Dnmt1, suggesting that the expression of Dnmt3a and Dnmt3b alone in ES cells is insufficient to achieve effective gene silencing.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(529)

We used a novel assay to show that missense mutation rates are also substantially reduced in Dnmt1 - deficient cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(530)

This is the first direct demonstration that DNA methylation affects point mutation rates in mammalian cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(531)

Surprisingly, the fraction of CpG transition mutations was not reduced in Dnmt1 - deficient cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(532)

Finally, we show that methyl group - deficient growth conditions do not cause an increase in missense mutation rates in Dnmt1 - proficient cells, as predicted by methyltransferase - mediated mutagenesis models.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(533)

We conclude that Dnmt1 deficiency and the accompanying genomic DNA hypomethylation result in a reduction of three major pathways of gene inactivation in our model system.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(534)

Induction of hepatitis C virus E1 envelope protein - specific immune response can be enhanced by mutation of N - glycosylation sites.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(535)

Deglycosylation of viral glycoproteins has been shown to influence the number of available epitopes and to modulate immune recognition of antigens.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(536)

We investigated the role played by N - glycans in the immunogenicity of hepatitis C virus ( HCV ) E1 envelope glycoprotein, a naturally poor immunogen.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(537)

Eight plasmids were engineered, encoding E1 protein mutants in which the four N - linked glycosylation sites of the protein were mutated separately or in combination.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(538)

In vitro expression studies showed an influence of N - linked glycosylation on expression efficiency, instability, and / or secretion of the mutated proteins.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Relation	Text (Head - Tail)
0.9529	Methylation	In vitro expression studies showed an influence of GlycosylationN - linked glycosylation Glycosylationglycosylation on expression efficiency, instability, and / or secretion of the mutated proteins.
0.9435	Methylation	In vitro expression studies showed an influence of GlycosylationN - linked glycosylation Glycosylationglycosylation on expression efficiency, instability, and / or secretion of the mutated proteins.

(539)

Immunogenicity of the E1 mutants was studied in BALB / c mice following intramuscular and intraepidermal injection of the plasmids.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(540)

Whereas some mutations had no or only minor effects on the antibody titers induced, mutation of the fourth glycosylation site ( N4 ) significantly enhanced the anti - E1 humoral response in terms of both seroconversion rates and antibody titers.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(541)

Moreover, antibody induced by the N4 mutant was able to recognize HCV - like particles with higher titers than those induced by the wild - type construct.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(542)

Epitope mapping indicated that the E1 mutant antigens induced antibody directed at two major domains : one, located at amino acids ( aa ) 313 to 332, which is known to be reactive with sera from HCV patients, and a second one, located in the N - terminal domain of E1 ( aa 192 to 226 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(543)

Analysis of the induced immune cellular response confirmed the induction of gamma interferon - producing cells by all mutants, albeit to different levels.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(544)

These results show that N - linked glycosylation can limit the antibody response to the HCV E1 protein and reveal a potential vaccine candidate with enhanced immunogenicity.

Precision: 11.11

Recall: 100.00

F1: 20.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	These results show that GlycosylationN - linked glycosylation can limit the antibody response to the HCV ProteinE1 protein and reveal a potential vaccine candidate with enhanced immunogenicity.
1.0000	Methylation	These results show that N - linked Glycosylationglycosylation can limit the antibody response to the HCV ProteinE1 protein and reveal a potential vaccine candidate with enhanced immunogenicity.
0.9965	Methylation	These results show that GlycosylationN - linked glycosylation can limit the antibody response to the HCV ProteinE1 protein and reveal a potential vaccine candidate with enhanced immunogenicity.
0.8548	Methylation	These results show that GlycosylationN - linked glycosylation Glycosylationglycosylation can limit the antibody response to the HCV E1 protein and reveal a potential vaccine candidate with enhanced immunogenicity.
0.8246	Methylation	These results show that GlycosylationN - linked glycosylation Glycosylationglycosylation can limit the antibody response to the HCV E1 protein and reveal a potential vaccine candidate with enhanced immunogenicity.
0.7682	Methylation	These results show that GlycosylationN - linked Glycosylationglycosylation can limit the antibody response to the HCV E1 protein and reveal a potential vaccine candidate with enhanced immunogenicity.
0.7531	Methylation	These results show that GlycosylationN - linked GlycosylationN - linked glycosylation can limit the antibody response to the HCV E1 protein and reveal a potential vaccine candidate with enhanced immunogenicity.
0.6236	Methylation	These results show that GlycosylationN - linked Glycosylationglycosylation can limit the antibody response to the HCV E1 protein and reveal a potential vaccine candidate with enhanced immunogenicity.
0.5492	Methylation	These results show that GlycosylationN - linked glycosylation GlycosylationN - linked glycosylation can limit the antibody response to the HCV E1 protein and reveal a potential vaccine candidate with enhanced immunogenicity.

(545)

Absence of post - translational aspartyl beta - hydroxylation of epidermal growth factor domains in mice leads to developmental defects and an increased incidence of intestinal neoplasia.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(546)

The BAH genomic locus encodes three distinct proteins : junctin, humbug, and BAH.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(547)

All three proteins share common exons, but differ significantly based upon the use of alternative terminal exons.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(548)

The biological roles of BAH and humbug and their functional relationship to junctin remain unclear.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(549)

To evaluate the role of BAH in vivo, the catalytic domain of BAH was specifically targeted such that the coding regions of junctin and humbug remained undisturbed.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(550)

BAH null mice lack measurable BAH protein in several tissues, lack aspartyl beta - hydroxylase activity in liver preparations, and exhibit no hydroxylation of the epidermal growth factor ( EGF ) domain of clotting Factor X.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Relation	Text (Head - Tail)
	Methylation	BAH null mice lack measurable BAH protein in several tissues, lack aspartyl beta - hydroxylase activity in liver preparations, and exhibit no Hydroxylationhydroxylation of the Entityepidermal growth factor ( EGF ) domain of clotting Factor X.

(551)

In addition to reduced fertility in females, BAH null mice display several developmental defects including syndactyly, facial dysmorphology, and a mild defect in hard palate formation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(552)

The developmental defects present in BAH null mice are similar to defects observed in knock - outs and hypomorphs of the Notch ligand Serrate - 2.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(553)

In this work, beta - hydroxylation of Asp residues in EGF domains is demonstrated for a soluble form of a Notch ligand, human Jagged - 1.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Relation	Text (Head - Tail)
0.9999	Methylation	In this work, Hydroxylationbeta - hydroxylation of EntityAsp residues in EGF domains is demonstrated for a soluble form of a Notch ligand, human Jagged - 1.
0.9997	Methylation	In this work, Hydroxylationbeta - hydroxylation of EntityAsp residues in EGF domains is demonstrated for a soluble form of a Notch ligand, human Jagged - 1.

(554)

These results along with recent reports that another post - translational modification of EGF domains in Notch gene family members ( glycosylation by Fringe ) alters Notch pathway signaling, lends credence to the suggestion that aspartyl beta - hydroxylation may represent another post - translational modification of EGF domains that can modulate Notch pathway signaling.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(555)

Previous work has demonstrated increased levels of BAH in certain tumor tissues and a role for BAH in tumorigenesis has been proposed.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(556)

The role of hydroxylase in tumor formation was tested directly by crossing BAH KO mice with an intestinal tumor model, APCmin mice.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(557)

Surprisingly, BAH null / APCmin mice show a statistically significant increase in both intestinal polyp size and number when compared with BAH wild - type / APCmin controls.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(558)

These results suggest that, in contrast to expectations, loss of BAH catalytic activity may promote tumor formation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(559)

Molecular cloning of ESET, a novel histone H3 - specific methyltransferase that interacts with ERG transcription factor.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(560)

The ets - related gene erg encodes a transcription factor that is implicated in the control of cell growth and differentiation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(561)

To identify interacting partners of ERG, we screened a yeast two - hybrid cDNA library constructed from mouse hematopoietic cells using the N - terminal region of ERG as a bait.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(562)

We isolated a 4. 6 kb full - length mouse cDNA encoding a 1307 - amino acid protein migrating as a 180 kD band, which was termed ESET ( ERG - associated protein with SET domain ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(563)

ESET is 92 % identical to the human protein SETDB1 ( SET domain, bifurcated 1 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(564)

The interaction between ESET and ERG was supported by in vitro pull - down using glutathione S - transferase ( GST ) fusion protein, by transfection and co - immunoprecipitation experiments, and by association of endogenous SETDB1 with ERG.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(565)

Since ESET possesses evolutionarily conserved SET, preSET, and postSET domains implicated in histone methylation, we tested the ability of ESET to methylate core histones.

Precision: 33.33

Recall: 100.00

F1: 50.00

Score	Relation	Text (Head - Tail)
0.9982	Methylation	Since ESET possesses evolutionarily conserved SET, preSET, and postSET domains implicated in Proteinhistone Methylationmethylation , we tested the ability of ESET to methylate core histones.
0.9999	Methylation	Since ESET possesses evolutionarily conserved SET, preSET, and postSET domains implicated in histone methylation, we tested the ability of ESET to Methylationmethylate core Proteinhistones .
0.7511	Methylation	Since ESET possesses evolutionarily conserved SET, preSET, and postSET domains implicated in histone methylation, we tested the ability of ProteinESET to Methylationmethylate core histones.

(566)

The results of these studies demonstrated that ESET is a histone H3 - specific methyltransferase, and that mutations within ESET abolished its methyltransferase activity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(567)

Together, these findings raise the possibility that transcription factor ERG may participate in transcriptional regulation through ESET - mediated histone methylation.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Relation	Text (Head - Tail)
0.9886	Methylation	Together, these findings raise the possibility that transcription factor ERG may participate in transcriptional regulation through ProteinESET - mediated histone Methylationmethylation .
0.5660	Methylation	Together, these findings raise the possibility that transcription factor ERG may participate in transcriptional regulation through ProteinESET - mediated histone ProteinESET - mediated histone methylation.
0.5367	Methylation	Together, these findings raise the possibility that transcription factor ERG may participate in transcriptional regulation through ProteinESET - mediated ProteinESET - mediated histone methylation.
	Methylation	Together, these findings raise the possibility that transcription factor ERG may participate in transcriptional regulation through ProteinESET - mediated ProteinESET - mediated histone methylation.
	Methylation	Together, these findings raise the possibility that transcription factor ERG may participate in transcriptional regulation through ESET - mediated Proteinhistone Methylationmethylation .

(568)

Regulation of HIF by the von Hippel - Lindau tumour suppressor : implications for cellular oxygen sensing.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(569)

Hypoxia - inducible factor ( HIF ) is central in coordinating many of the transcriptional adaptations to hypoxia.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(570)

Composed of a heterodimer of alpha and beta subunits, the alpha subunit is rapidly degraded in normoxia, leading to inactivation of the hypoxic response.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(571)

Many models for a molecular oxygen sensor regulating this system have been proposed, but an important finding has been the ability to mimic hypoxia by chelation or substitution of iron.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(572)

A key insight has been the recognition that HIF - alpha is targeted for degradation by the ubiquitin - proteasome pathway through binding to the von Hippel - Lindau tumour suppressor protein ( pVHL ), which forms the recognition component of an E3 ubiquitin ligase complex leading to ubiquitylation of HIF - alpha.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(573)

Importantly, the classical features of regulation by iron and oxygen availability are reflected in regulation of the HIF - alpha / pVHL interaction.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(574)

It has recently been shown that HIF - alpha undergoes an iron - and oxygen - dependent modification before it can interact with pVHL, and that this results in hydroxylation of at least one prolyl residue ( HIF - 1alpha, Pro 564 ).

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Relation	Text (Head - Tail)
0.9999	Methylation	It has recently been shown that HIF - alpha undergoes an iron - and oxygen - dependent modification before it can interact with pVHL, and that this results in Hydroxylationhydroxylation of at least one Entityprolyl residue ( HIF - 1alpha, Pro 564 ).
0.9998	Methylation	It has recently been shown that HIF - alpha undergoes an iron - and oxygen - dependent modification before it can interact with pVHL, and that this results in Hydroxylationhydroxylation of at least one Entityprolyl residue ( HIF - 1alpha, Pro 564 ).

(575)

This modification is catalysed by an enzyme termed HIF - prolyl hydroxylase ( HIF - PH ), and compatible with all previously described prolyl - 4 - hydroxylases HIF - PH also requires 2 - oxoglutarate as a cosubstrate.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(576)

The key position of this hydroxylation in the degradation pathway of HIF - alpha, together with its requirement for molecular dioxygen as a co - substrate, provides the potential for HIF - PH to function directly as a cellular oxygen sensor.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(577)

However, the ability of these enzyme ( s ) to account for the full range of physiological regulation displayed by the HIF system remains to be defined.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(578)

Promoter methylation status of the DNA repair genes hMLH1 and MGMT in gastric carcinoma and metaplastic mucosa.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9993	Methylation	EntityPromoter DNA_methylationmethylation status of the DNA repair genes hMLH1 and MGMT in gastric carcinoma and metaplastic mucosa.

(579)

Hypermethylation of CpG islands in the promoter region is associated with the silencing of a variety of tumor suppressor genes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(580)

DNA repair genes human Mut L homologue 1 ( hMLH1 ) and O ( 6 ) - methylguanine - DNA methyltransferase ( MGMT ) have been shown to be hypermethylated in certain carcinomas.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9956	Methylation	DNA repair genes human Mut L homologue 1 ( hMLH1 ) and ProteinO ( 6 ) - methylguanine - DNA methyltransferase ( MGMT ) have been shown to be DNA_methylationhypermethylated in certain carcinomas.
0.9864	Methylation	DNA repair genes human ProteinMut L homologue 1 ( hMLH1 ) and O ( 6 ) - methylguanine - DNA methyltransferase ( MGMT ) have been shown to be DNA_methylationhypermethylated in certain carcinomas.

(581)

We studied DNA methylation of CpG islands in hMLH1 and MGMT in 50 gastric carcinomas and 10 intestinal metaplastic mucosa samples.

Precision: 33.33

Recall: 100.00

F1: 50.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	We studied DNA_methylationDNA methylation of EntityCpG islands in hMLH1 and MGMT in 50 gastric carcinomas and 10 intestinal metaplastic mucosa samples.
1.0000	Methylation	We studied DNA DNA_methylationmethylation of EntityCpG islands in hMLH1 and MGMT in 50 gastric carcinomas and 10 intestinal metaplastic mucosa samples.
0.9745	Methylation	We studied DNA_methylationDNA methylation of EntityCpG islands in hMLH1 and MGMT in 50 gastric carcinomas and 10 intestinal metaplastic mucosa samples.

(582)

We analyzed the methylation status of hMLH1 and MGMT using methylation - specific polymerase chain reaction and DNA sequencing analysis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	We analyzed the DNA_methylationmethylation status of ProteinhMLH1 and MGMT using methylation - specific polymerase chain reaction and DNA sequencing analysis.
0.9998	Methylation	We analyzed the DNA_methylationmethylation status of hMLH1 and ProteinMGMT using methylation - specific polymerase chain reaction and DNA sequencing analysis.

(583)

We measured protein levels of hMLH1 using Western blot and immunohistochemical analysis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(584)

CpG island hypermethylation of hMLH1 and MGMT was detected in 11 ( 22 % ) and 8 ( 16 % ) of the 50 gastric tumors, respectively.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9906	Methylation	EntityCpG island DNA_methylationhypermethylation of hMLH1 and MGMT was detected in 11 ( 22 % ) and 8 ( 16 % ) of the 50 gastric tumors, respectively.

(585)

Hypermethylation of the promoter was more common in intestinal - type gastric carcinomas than in poorly diffuse - type gastric carcinomas ( p = 0. 016 and 0. 021, respectively ; Fisher's exact test ).

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Relation	Text (Head - Tail)
0.9989	Methylation	DNA_methylationHypermethylation of the Entitypromoter was more common in intestinal - type gastric carcinomas than in poorly diffuse - type gastric carcinomas ( p = 0. 016 and 0. 021, respectively ; Fisher's exact test ).

(586)

However, hMLH1 promoter hypermethylation did not coincide with MGMT promoter hypermethylation except in 1 patient.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9955	Methylation	However, hMLH1 Entitypromoter DNA_methylationhypermethylation did not coincide with MGMT promoter hypermethylation except in 1 patient.
0.9932	Methylation	However, hMLH1 promoter hypermethylation did not coincide with MGMT Entitypromoter DNA_methylationhypermethylation except in 1 patient.

(587)

Hypermethylation of the hMLH1 promoter but not the MGMT promoter occurred in intestinal metaplastic mucosae.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9976	Methylation	DNA_methylationHypermethylation of the hMLH1 Entitypromoter but not the MGMT promoter occurred in intestinal metaplastic mucosae.
0.9921	Methylation	DNA_methylationHypermethylation of the hMLH1 promoter but not the MGMT Entitypromoter occurred in intestinal metaplastic mucosae.

(588)

Immunohistochemical analysis revealed a corresponding reduction in hMLH1 protein expression in some of the intestinal metaplastic mucosae.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(589)

Our results suggest that at least two types of promoter methylation participate in the development of gastric carcinoma.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(590)

Tumor - specific promoter hypermethylation of hMLH1 may be an early event in carcinogenesis in the stomach.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9972	Methylation	Tumor - specific Entitypromoter DNA_methylationhypermethylation of hMLH1 may be an early event in carcinogenesis in the stomach.

(591)

Histone deacetylases and cancer : causes and therapies.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(592)

Together, histone acetyltransferases and histone deacetylases ( HDACs ) determine the acetylation status of histones.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	Together, histone acetyltransferases and histone deacetylases ( HDACs ) determine the Acetylationacetylation status of Proteinhistones .

(593)

This acetylation affects the regulation of gene expression, and inhibitors of HDACs have been found to cause growth arrest, differentiation and / or apoptosis of many tumours cells by altering the transcription of a small number of genes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(594)

HDAC inhibitors are proving to be an exciting therapeutic approach to cancer, but how do they exert this effect?

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(595)

Co - and posttranslational modification of the alpha ( 1B ) - adrenergic receptor : effects on receptor expression and function.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(596)

We have characterized the maturation, co - and posttranslational modifications, and functional properties of the alpha ( 1B ) - adrenergic receptor ( AR ) expressed in different mammalian cells transfected using conventional approaches or the Semliki Forest virus system.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(597)

We found that the alpha ( 1B ) - AR undergoes N - linked glycosylation as demonstrated by its sensitivity to endoglycosidases and by the effect of tunicamycin on receptor maturation.

Precision: 11.11

Recall: 100.00

F1: 20.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	We found that the Proteinalpha ( 1B ) - AR undergoes GlycosylationN - linked glycosylation as demonstrated by its sensitivity to endoglycosidases and by the effect of tunicamycin on receptor maturation.
0.9999	Methylation	We found that the Proteinalpha ( 1B ) - AR undergoes GlycosylationN - linked glycosylation as demonstrated by its sensitivity to endoglycosidases and by the effect of tunicamycin on receptor maturation.
0.9997	Methylation	We found that the Proteinalpha ( 1B ) - AR undergoes N - linked Glycosylationglycosylation as demonstrated by its sensitivity to endoglycosidases and by the effect of tunicamycin on receptor maturation.
0.8658	Methylation	We found that the alpha ( 1B ) - AR undergoes GlycosylationN - linked glycosylation Glycosylationglycosylation as demonstrated by its sensitivity to endoglycosidases and by the effect of tunicamycin on receptor maturation.
0.8465	Methylation	We found that the alpha ( 1B ) - AR undergoes GlycosylationN - linked glycosylation Glycosylationglycosylation as demonstrated by its sensitivity to endoglycosidases and by the effect of tunicamycin on receptor maturation.
0.8410	Methylation	We found that the alpha ( 1B ) - AR undergoes GlycosylationN - linked Glycosylationglycosylation as demonstrated by its sensitivity to endoglycosidases and by the effect of tunicamycin on receptor maturation.
0.8319	Methylation	We found that the alpha ( 1B ) - AR undergoes GlycosylationN - linked GlycosylationN - linked glycosylation as demonstrated by its sensitivity to endoglycosidases and by the effect of tunicamycin on receptor maturation.
0.5566	Methylation	We found that the alpha ( 1B ) - AR undergoes GlycosylationN - linked glycosylation GlycosylationN - linked glycosylation as demonstrated by its sensitivity to endoglycosidases and by the effect of tunicamycin on receptor maturation.
0.5341	Methylation	We found that the alpha ( 1B ) - AR undergoes GlycosylationN - linked Glycosylationglycosylation as demonstrated by its sensitivity to endoglycosidases and by the effect of tunicamycin on receptor maturation.

(598)

Pulse - chase labeling experiments in BHK - 21 cells demonstrate that the alpha ( 1B ) - AR is synthesized as a 70 kDa core glycosylated precursor that is converted to the 90 kDa mature form of the receptor with a half - time of approximately 2 h.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	Pulse - chase labeling experiments in BHK - 21 cells demonstrate that the Proteinalpha ( 1B ) - AR is synthesized as a 70 kDa core Glycosylationglycosylated precursor that is converted to the 90 kDa mature form of the receptor with a half - time of approximately 2 h.

(599)

N - Linked glycosylation of the alpha ( 1B ) - AR occurs at four asparagines on the N - terminus of the receptor.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Relation	Text (Head - Tail)
0.9977	Methylation	N - Linked Glycosylationglycosylation of the alpha ( 1B ) - AR occurs at Entityfour asparagines on the N - terminus of the receptor.
0.9988	Methylation	N - Linked Glycosylationglycosylation of the alpha ( 1B ) - AR occurs at four Entityasparagines on the N - terminus of the receptor.

(600)

Mutations of the N - linked glycosylation sites did not have a significant effect on receptor function or expression.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(601)

Surprisingly, receptor mutants lacking N - linked glycosylation migrated as heterogeneous bands in SDS - PAGE.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(602)

Our findings demonstrate that N - linked glycosylation and phosphorylation, but not palmitoylation or O - linked glycosylation, contribute to the structural heterogeneity of the alpha ( 1B ) - AR as it is observed in SDS - PAGE.

Precision: 16.67

Recall: 100.00

F1: 28.57

Score	Relation	Text (Head - Tail)
1.0000	Methylation	Our findings demonstrate that N - linked glycosylation and phosphorylation, but not palmitoylation or GlycosylationO - linked glycosylation , contribute to the structural heterogeneity of the Proteinalpha ( 1B ) - AR as it is observed in SDS - PAGE.
0.9993	Methylation	Our findings demonstrate that GlycosylationN - linked glycosylation and phosphorylation, but not palmitoylation or O - linked glycosylation, contribute to the structural heterogeneity of the Proteinalpha ( 1B ) - AR as it is observed in SDS - PAGE.
0.9977	Methylation	Our findings demonstrate that N - linked glycosylation and Phosphorylationphosphorylation , but not palmitoylation or O - linked glycosylation, contribute to the structural heterogeneity of the Proteinalpha ( 1B ) - AR as it is observed in SDS - PAGE.
1.0000	Methylation	Our findings demonstrate that N - linked glycosylation and phosphorylation, but not palmitoylation or O - linked Glycosylationglycosylation , contribute to the structural heterogeneity of the Proteinalpha ( 1B ) - AR as it is observed in SDS - PAGE.
0.9999	Methylation	Our findings demonstrate that N - linked glycosylation and phosphorylation, but not palmitoylation or GlycosylationO - linked glycosylation, contribute to the structural heterogeneity of the Proteinalpha ( 1B ) - AR as it is observed in SDS - PAGE.
0.9993	Methylation	Our findings demonstrate that N - linked Glycosylationglycosylation and phosphorylation, but not palmitoylation or O - linked glycosylation, contribute to the structural heterogeneity of the Proteinalpha ( 1B ) - AR as it is observed in SDS - PAGE.
0.9981	Methylation	Our findings demonstrate that GlycosylationN - linked glycosylation and phosphorylation, but not palmitoylation or O - linked glycosylation, contribute to the structural heterogeneity of the Proteinalpha ( 1B ) - AR as it is observed in SDS - PAGE.
0.7844	Methylation	Our findings demonstrate that N - linked glycosylation and phosphorylation, but not palmitoylation or GlycosylationO - linked glycosylation Glycosylationglycosylation , contribute to the structural heterogeneity of the alpha ( 1B ) - AR as it is observed in SDS - PAGE.
0.6685	Methylation	Our findings demonstrate that N - linked glycosylation and phosphorylation, but not palmitoylation or GlycosylationO - linked glycosylation Glycosylationglycosylation , contribute to the structural heterogeneity of the alpha ( 1B ) - AR as it is observed in SDS - PAGE.
0.6467	Methylation	Our findings demonstrate that GlycosylationN - linked glycosylation Glycosylationglycosylation and phosphorylation, but not palmitoylation or O - linked glycosylation, contribute to the structural heterogeneity of the alpha ( 1B ) - AR as it is observed in SDS - PAGE.
0.5924	Methylation	Our findings demonstrate that GlycosylationN - linked glycosylation Glycosylationglycosylation and phosphorylation, but not palmitoylation or O - linked glycosylation, contribute to the structural heterogeneity of the alpha ( 1B ) - AR as it is observed in SDS - PAGE.
0.5883	Methylation	Our findings demonstrate that N - linked glycosylation and phosphorylation, but not palmitoylation or GlycosylationO - linked Glycosylationglycosylation , contribute to the structural heterogeneity of the alpha ( 1B ) - AR as it is observed in SDS - PAGE.
0.5019	Methylation	Our findings demonstrate that GlycosylationN - linked GlycosylationN - linked glycosylation and phosphorylation, but not palmitoylation or O - linked glycosylation, contribute to the structural heterogeneity of the alpha ( 1B ) - AR as it is observed in SDS - PAGE.
0.4980	Methylation	Our findings demonstrate that GlycosylationN - linked Glycosylationglycosylation and phosphorylation, but not palmitoylation or O - linked glycosylation, contribute to the structural heterogeneity of the alpha ( 1B ) - AR as it is observed in SDS - PAGE.
0.4769	Methylation	Our findings demonstrate that N - linked glycosylation and phosphorylation, but not palmitoylation or GlycosylationO - linked GlycosylationO - linked glycosylation , contribute to the structural heterogeneity of the alpha ( 1B ) - AR as it is observed in SDS - PAGE.
0.4698	Methylation	Our findings demonstrate that N - linked glycosylation and phosphorylation, but not palmitoylation or GlycosylationO - linked glycosylation GlycosylationO - linked glycosylation, contribute to the structural heterogeneity of the alpha ( 1B ) - AR as it is observed in SDS - PAGE.
0.4490	Methylation	Our findings demonstrate that N - linked glycosylation and phosphorylation, but not palmitoylation or GlycosylationO - linked Glycosylationglycosylation , contribute to the structural heterogeneity of the alpha ( 1B ) - AR as it is observed in SDS - PAGE.
0.4099	Methylation	Our findings demonstrate that GlycosylationN - linked Glycosylationglycosylation and phosphorylation, but not palmitoylation or O - linked glycosylation, contribute to the structural heterogeneity of the alpha ( 1B ) - AR as it is observed in SDS - PAGE.

(603)

The modifications found are similar in the different mammalian expression systems explored.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(604)

Our findings indicate that the Semliki Forest virus system can provide large amounts of functional and fully glycosylated alpha ( 1B ) - AR protein suitable for biochemical and structural studies.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9997	Methylation	Our findings indicate that the Semliki Forest virus system can provide large amounts of functional and fully Glycosylationglycosylated Proteinalpha ( 1B ) - AR protein suitable for biochemical and structural studies.

(605)

The results of this study contribute to elucidate the basic steps involved in the processing of G protein - coupled receptors as well as to optimize strategies for their overexpression.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(606)

Binding mode analysis of 3 - ( 4 - benzoyl - 1 - methyl - 1H - 2 - pyrrolyl ) - N - hydroxy - 2 - propenamide : a new synthetic histone deacetylase inhibitor inducing histone hyperacetylation, growth inhibition, and terminal cell differentiation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	Binding mode analysis of 3 - ( 4 - benzoyl - 1 - methyl - 1H - 2 - pyrrolyl ) - N - hydroxy - 2 - propenamide : a new synthetic histone deacetylase inhibitor inducing Proteinhistone Acetylationhyperacetylation , growth inhibition, and terminal cell differentiation.

(607)

The binding mode of 3 - ( 4 - aroyl - 1H - 2 - pyrrolyl ) - N - hydroxy - 2 - propenamides 1a - c, belonging to a recently reported class of synthetic histone deacetylase ( HDAC ) inhibitors ( Massa, S. ; et al.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(608)

J.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(609)

Med.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(610)

Chem. 2001, 44, 2069 - 2072 ), into the new modeled HDAC1 catalytic core is presented, and enzyme / inhibitor interactions are discussed.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(611)

HDAC1 X - ray coordinates were obtained by virtual " mutation " of those of histone deacetylase - like protein, a bacterial HDAC homologue.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(612)

In in vitro antimaize HD2 as well as antimouse HDAC1 assay, compounds 1a - c showed inhibitory activities in the low micromolar range.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(613)

Similarly, 1a - c are endowed with anti - HDAC activity in vivo : on mouse A20 cells, 1a - c induced histone hyperacetylation leading to a highly increased acetylation level of H4 as compared to control histones.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9997	Methylation	Similarly, 1a - c are endowed with anti - HDAC activity in vivo : on mouse A20 cells, 1a - c induced histone hyperacetylation leading to a highly increased Acetylationacetylation level of ProteinH4 as compared to control histones.
0.9979	Methylation	Similarly, 1a - c are endowed with anti - HDAC activity in vivo : on mouse A20 cells, 1a - c induced Proteinhistone Acetylationhyperacetylation leading to a highly increased acetylation level of H4 as compared to control histones.

(614)

Results obtained with acid - urea - triton polyacrylamide gel electrophoresis have been confirmed by Western Blot experiments.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(615)

Finally, compound 1a, chosen as a representative member of this class of HDAC inhibitors, resulted endowed with antiproliferative ( 45 and 85 % cell growth inhibition at 40 and 80 microM, respectively ) and cellular differentiation ( 18 and 21 % of benzidine positive cells at the same concentrations ) activities in murine erythroleukemic cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(616)

Hypoxia - inducible factor ( HIF ) asparagine hydroxylase is identical to factor inhibiting HIF ( FIH ) and is related to the cupin structural family.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(617)

Activity of the hypoxia - inducible factor ( HIF ) complex is controlled by oxygen - dependent hydroxylation of prolyl and asparaginyl residues.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(618)

Hydroxylation of specific prolyl residues by 2 - oxoglutarate ( 2 - OG ) - dependent oxygenases mediates ubiquitinylation and proteasomal destruction of HIF - alpha.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(619)

Hydroxylation of an asparagine residue in the C - terminal transactivation domain ( CAD ) of HIF - alpha abrogates interaction with p300, preventing transcriptional activation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(620)

Yeast two - hybrid assays recently identified factor inhibiting HIF ( FIH ) as a protein that associates with the CAD region of HIF - alpha.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(621)

Since FIH contains certain motifs present in iron - and 2 - OG - dependent oxygenases we investigated whether FIH was the HIF asparaginyl hydroxylase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(622)

Assays using recombinant FIH and HIF - alpha fragments revealed that FIH is the enzyme that hydroxylates the CAD asparagine residue, that the activity is directly inhibited by cobalt ( II ) and limited by hypoxia, and that the oxygen in the alcohol of the hydroxyasparagine residue is directly derived from dioxygen.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(623)

Sequence analyses involving FIH link the 2 - OG oxygenases with members of the cupin superfamily, including Zn ( II ) - utilizing phosphomannose isomerase, revealing structural and evolutionary links between these metal - binding proteins that share common motifs.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(624)

FIH - 1 is an asparaginyl hydroxylase enzyme that regulates the transcriptional activity of hypoxia - inducible factor.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(625)

Mammalian cells adapt to hypoxic conditions through a transcriptional response pathway mediated by the hypoxia - inducible factor, HIF.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(626)

HIF transcriptional activity is suppressed under normoxic conditions by hydroxylation of an asparagine residue within its C - terminal transactivation domain, blocking association with coactivators.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(627)

Here we show that the protein FIH - 1, previously shown to interact with HIF, is an asparaginyl hydroxylase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(628)

Like known hydroxylase enzymes, FIH - 1 is an Fe ( II ) - dependent enzyme that uses molecular O ( 2 ) to modify its substrate.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(629)

Together with the recently discovered prolyl hydroxylases that regulate HIF stability, this class of oxygen - dependent enzymes comprises critical regulatory components of the hypoxic response pathway.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(630)

[ alpha ] - Secretase ADAM10 as well as [ alpha ] APPs is reduced in platelets and CSF of Alzheimer disease patients.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(631)

BACKGROUND : Members of membrane - bound disintegrin metalloproteinases ( ADAMs ) were shown to be capable of cleaving amyloid precursor protein ( APP ) at the alpha - cleavage site in different cell systems.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(632)

One of the candidate alpha - secretases identified in this family is ADAM10.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(633)

The present study addresses the following major questions : 1 ) Are the levels of an alpha - secretase candidate ( i. e., ADAM10 ) reduced in accessible cells of Alzheimer Disease ( AD ) patients? 2 ) Are ADAM10 levels in the peripheral cells of AD patients related to a concomitant decrease in alpha APPs? MATERIALS AND METHODS : Western Blot analysis of ADAM10 is performed on platelet homogenates from 33 sporadic AD patients and on 26 age - matched control subjects.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(634)

Moreover, the levels of alpha - secretase metabolite ( alpha APPs ) are tested both in platelets and cerebrospinal fluid ( CSF ) of the same pool of subjects by means of Western blot with a specific antibody.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(635)

RESULTS : A significant decrease of platelet ADAM10 levels is observed in patients affected by probable AD when compared to control subjects and this is paralleled by a reduced level of alpha APPs released from platelets.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(636)

Moreover, in the same pool of AD patients, alpha APPs levels were reduced concomitantly in CSF.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(637)

CONCLUSIONS : ADAM10 is expressed in platelets.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(638)

A reduced level of ADAM10 is observed in platelets obtained from AD patients compared to age - matched controls.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(639)

Further, in the same pool of AD patients, a qualitatively and quantitatively similar decrease in alpha APPs is present both in thrombin - activated platelets and CSF, thus suggesting that alterations of APP processing might occur both in the neuronal compartment and peripheral cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(640)

Reduced expression of the insulin - induced protein 1 and p41 Arp2 / 3 complex genes in human gastric cancers.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(641)

Aberrantly methylated DNA fragments in a human gastric cancer were searched for by a genome - scanning method, methylation - sensitive - representational difference analysis ( MS - RDA ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(642)

Six DNA fragments flanked by CpG islands ( CGIs ) and hypermethylated in the cancer were isolated.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(643)

Four of the 6 fragments possessed genes in their vicinities.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(644)

Quantitative RT - PCR analysis of the 4 genes showed reduced expression of 2 genes in cancers : Insulin - induced protein 1 ( INSIG1 / CL - 6 ) and p41 Arp2 / 3 complex ( p41 - Arc ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(645)

As for INSIG1, a DNA fragment was derived from the edge of a CGI in the promoter region.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(646)

The edge was methylated in 11 of 22 primary gastric cancers, whereas the center was not methylated in any cancer.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	The edge was methylated in 11 of 22 primary gastric cancers, whereas the Entitycenter was not DNA_methylationmethylated in any cancer.
0.9993	Methylation	The Entityedge was DNA_methylationmethylated in 11 of 22 primary gastric cancers, whereas the center was not methylated in any cancer.

(647)

INSIG1 expression was markedly reduced in 19 cancers, including the 11 cancers with the methylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9993	Methylation	ProteinINSIG1 expression was markedly reduced in 19 cancers, including the 11 cancers with the DNA_methylationmethylation .

(648)

By 5 - aza - 2'- deoxycytidine treatment of 5 cell lines with the methylation of the edge, partial restoration of INSIG1 expression was detected only in 2 of them.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	By 5 - aza - 2'- deoxycytidine treatment of 5 cell lines with the DNA_methylationmethylation of the Entityedge , partial restoration of INSIG1 expression was detected only in 2 of them.

(649)

These data indicated that, although the reduced INSIG1 expression in cancers was associated with the methylation at the edge of the CGI in the promoter region, the methylation was likely to be a secondary change.

Precision: 25.00

Recall: 100.00

F1: 40.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	These data indicated that, although the reduced INSIG1 expression in cancers was associated with the DNA_methylationmethylation at the Entityedge of the CGI in the promoter region, the methylation was likely to be a secondary change.
0.9960	Methylation	These data indicated that, although the reduced INSIG1 expression in cancers was associated with the DNA_methylationmethylation at the edge of the EntityCGI in the promoter region, the methylation was likely to be a secondary change.
0.7434	Methylation	These data indicated that, although the reduced INSIG1 expression in cancers was associated with the methylation at the Entityedge of the CGI in the promoter region, the DNA_methylationmethylation was likely to be a secondary change.
0.6073	Methylation	These data indicated that, although the reduced INSIG1 expression in cancers was associated with the methylation at the edge of the EntityCGI in the promoter region, the DNA_methylationmethylation was likely to be a secondary change.

(650)

As for p41 - Arc, a DNA fragment was derived from a CGI overlapping exon 8, and its methylation did not correlate with its expression.

Precision: 33.33

Recall: 100.00

F1: 50.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	As for p41 - Arc, a DNA fragment was derived from a EntityCGI overlapping exon 8 , and its DNA_methylationmethylation did not correlate with its expression.
1.0000	Methylation	As for p41 - Arc, a DNA fragment was derived from a CGI overlapping Entityexon 8 , and its DNA_methylationmethylation did not correlate with its expression.
1.0000	Methylation	As for p41 - Arc, a DNA fragment was derived from a CGI Entityoverlapping exon 8 , and its DNA_methylationmethylation did not correlate with its expression.

(651)

However, methylation of a CGI in the promoter region with a marked reduction of its expression was observed in 1 of the 22 primary cancers.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9996	Methylation	However, DNA_methylationmethylation of a EntityCGI in the promoter region with a marked reduction of its expression was observed in 1 of the 22 primary cancers.

(652)

INSIG1 and p41 - Arc are known to be involved in cellular differentiation and morphology, respectively, and it was suggested that their reduced expressions might be involved in gastric cancer development or progression.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(653)

The kyphoscoliotic type of Ehlers - Danlos syndrome ( type VI ) : differential effects on the hydroxylation of lysine in collagens I and II revealed by analysis of cross - linked telopeptides from urine.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	The kyphoscoliotic type of Ehlers - Danlos syndrome ( type VI ) : differential effects on the Hydroxylationhydroxylation of Entitylysine in collagens I and II revealed by analysis of cross - linked telopeptides from urine.

(654)

The kyphoscoliotic type of Ehlers - Danlos syndrome ( EDS type VIA ) ( OMIM 225400 ) is an autosomal recessive connective tissue disorder that results from mutations in the lysyl hydroxylase 1 gene ( PLOD1 ) causing underhydroxylation of lysine residues in tissue collagens, particularly of skin.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(655)

Previous studies have shown that the pool of collagen cross - linking amino acids, hydroxylysyl pyridinoline ( HP ) and lysyl pyridinoline ( LP ) excreted in urine has an abnormally low HP / LP ratio, which is diagnostic of the condition.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(656)

Here we isolated cross - linked peptides containing these residues from the urine of a child with EDS VIA homozygous for a mutation that results in a stop codon and effective null expression of PLOD1 enzyme activity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(657)

Peptides that had originated from bone type I collagen and cartilage type II collagen were identified.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(658)

A cross - linked N - telopeptide fraction that is derived from bone type I collagen contained only LP, no HP, which means that the helical lysines at residues 930 of alpha 1 ( I ) and 933 of alpha 2 ( I ) of the collagen triple - helix had not been hydroxylated.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Relation	Text (Head - Tail)
0.9986	Methylation	A cross - linked N - telopeptide fraction that is derived from bone type I collagen contained only LP, no HP, which means that the helical lysines at residues 930 of alpha 1 ( I ) and Entity933 of alpha 2 ( I ) of the collagen triple - helix had not been Hydroxylationhydroxylated .
0.9975	Methylation	A cross - linked N - telopeptide fraction that is derived from bone type I collagen contained only LP, no HP, which means that the helical lysines at Entityresidues 930 of alpha 1 ( I ) and 933 of alpha 2 ( I ) of the collagen triple - helix had not been Hydroxylationhydroxylated .
0.9976	Methylation	A cross - linked N - telopeptide fraction that is derived from bone type I collagen contained only LP, no HP, which means that the helical lysines at residues Entity930 of alpha 1 ( I ) and 933 of alpha 2 ( I ) of the collagen triple - helix had not been Hydroxylationhydroxylated .
0.9931	Methylation	A cross - linked N - telopeptide fraction that is derived from bone type I collagen contained only LP, no HP, which means that the helical Entitylysines at residues 930 of alpha 1 ( I ) and 933 of alpha 2 ( I ) of the collagen triple - helix had not been Hydroxylationhydroxylated .

(659)

The equivalent peptide fraction from a normal child's urine gave a ratio of HP to LP of 1. 5 : 1 typical for normal bone collagen.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(660)

A second cross - linked peptide that is derived from the C - telopeptide domain of cartilage type II collagen showed both HP and LP in a 2 : 1 ratio, compared with 18 : 1 for the equivalent peptide from a normal child's urine.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(661)

The results show that in EDS VIA, bone type I collagen is more markedly underhydroxylated than cartilage type II collagen, at least at those helical sites that form cross - links.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Relation	Text (Head - Tail)
0.9991	Methylation	The results show that in EDS VIA, bone type I collagen is more markedly Glycosylationunderhydroxylated than cartilage Proteintype II collagen , at least at those helical sites that form cross - links.
	Methylation	The results show that in EDS VIA, bone type I collagen is more markedly Hydroxylationunderhydroxylated than cartilage Proteintype II collagen , at least at those helical sites that form cross - links.

(662)

The residual fraction of HP found in the urine of EDS VI patients therefore appears to be contributed in significant part by the degradation products of cartilage.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(663)

Since PLOD1 is null, other PLOD genes must be responsible for the helical hydroxylation activity that results in HP.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(664)

The presented approach of analyzing urinary cross - linked C - telopeptide fragments of type II collagen may allow the detection of chondrodysplasias due to genetic defects in lysyl hydroxylase isoforms active in cartilage.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(665)

Postsynthetic trimethylation of histone H4 at lysine 20 in mammalian tissues is associated with aging.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9965	Methylation	Postsynthetic Methylationtrimethylation of histone H4 at Entitylysine 20 in mammalian tissues is associated with aging.

(666)

Methylation of the N - terminal region of histones was first described more than 35 years ago, but its biological significance has remained unclear.

Precision: 25.00

Recall: 100.00

F1: 40.00

Score	Relation	Text (Head - Tail)
0.9995	Methylation	MethylationMethylation of the EntityN - terminal region of histones was first described more than 35 years ago, but its biological significance has remained unclear.
0.9994	Methylation	MethylationMethylation of the EntityN - terminal region of histones was first described more than 35 years ago, but its biological significance has remained unclear.
0.5032	Methylation	Methylation of the EntityN - terminal EntityN - terminal region of histones was first described more than 35 years ago, but its biological significance has remained unclear.
0.4565	Methylation	Methylation of the EntityN - terminal region EntityN - terminal region of histones was first described more than 35 years ago, but its biological significance has remained unclear.

(667)

Proposed functions range from transcriptional regulation to the higher order packing of chromatin in progress of mitotic condensation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(668)

Primarily because of the recent discovery of the SET domain - depending H3 - specific histone methyltransferases SUV39H1 and Suv39h1, which selectively methylate lysine 9 of the H3 N terminus, this posttranslational modification has regained scientific interest.

Precision: 75.00

Recall: 100.00

F1: 85.71

Score	Relation	Text (Head - Tail)
0.9998	Methylation	Primarily because of the recent discovery of the SET domain - depending H3 - specific histone methyltransferases SUV39H1 and ProteinSuv39h1 , which selectively Methylationmethylate lysine 9 of the H3 N terminus, this posttranslational modification has regained scientific interest.
0.9987	Methylation	Primarily because of the recent discovery of the SET domain - depending H3 - specific histone methyltransferases ProteinSUV39H1 and Suv39h1, which selectively Methylationmethylate lysine 9 of the H3 N terminus, this posttranslational modification has regained scientific interest.
0.9927	Methylation	Primarily because of the recent discovery of the SET domain - depending H3 - specific histone methyltransferases SUV39H1 and Suv39h1, which selectively Methylationmethylate Entitylysine 9 of the H3 N terminus, this posttranslational modification has regained scientific interest.
0.9733	Methylation	Primarily because of the recent discovery of the SET domain - depending H3 - specific histone methyltransferases SUV39H1 and Suv39h1, which selectively Methylationmethylate Entitylysine 9 of the H3 N terminus, this posttranslational modification has regained scientific interest.

(669)

In the past, investigations concerning the biological significance of histone methylation were largely limited because of a lack of simple and sensitive analytical procedures for detecting this modification.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	In the past, investigations concerning the biological significance of Proteinhistone Methylationmethylation were largely limited because of a lack of simple and sensitive analytical procedures for detecting this modification.

(670)

The present work investigated the methylation pattern of histone H4 both in different mammalian organs of various ages and in cell lines by applying mass spectrometric analysis and a newly developed hydrophilic - interaction liquid chromatographic method enabling the simultaneous separation of methylated and acetylated forms, which obviates the need to work with radioactive materials.

Precision: 33.33

Recall: 100.00

F1: 50.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	The present work investigated the Methylationmethylation pattern of Proteinhistone H4 both in different mammalian organs of various ages and in cell lines by applying mass spectrometric analysis and a newly developed hydrophilic - interaction liquid chromatographic method enabling the simultaneous separation of methylated and acetylated forms, which obviates the need to work with radioactive materials.
0.7415	Methylation	The present work investigated the methylation pattern of Proteinhistone H4 both in different mammalian organs of various ages and in cell lines by applying mass spectrometric analysis and a newly developed hydrophilic - interaction liquid chromatographic method enabling the simultaneous separation of methylated and Acetylationacetylated forms, which obviates the need to work with radioactive materials.
0.5063	Methylation	The present work investigated the methylation pattern of Proteinhistone H4 both in different mammalian organs of various ages and in cell lines by applying mass spectrometric analysis and a newly developed hydrophilic - interaction liquid chromatographic method enabling the simultaneous separation of Methylationmethylated and acetylated forms, which obviates the need to work with radioactive materials.

(671)

In rat kidney and liver the dimethylated lysine 20 was found to be the main methylation product, whereas the monomethyl derivative was present in much smaller amounts.

Precision: 33.33

Recall: 100.00

F1: 50.00

Score	Relation	Text (Head - Tail)
0.9987	Methylation	In rat kidney and liver the Methylationdimethylated Entitylysine 20 was found to be the main methylation product, whereas the monomethyl derivative was present in much smaller amounts.
0.9987	Methylation	In rat kidney and liver the dimethylated Entitylysine 20 was found to be the main methylation product, whereas the Methylationmonomethyl derivative was present in much smaller amounts.
0.9999	Methylation	In rat kidney and liver the dimethylated Entitylysine 20 was found to be the main Methylationmethylation product, whereas the monomethyl derivative was present in much smaller amounts.
0.9964	Methylation	In rat kidney and liver the dimethylated Entitylysine 20 was found to be the main Methylationmethylation product, whereas the monomethyl derivative was present in much smaller amounts.
0.9956	Methylation	In rat kidney and liver the Methylationdimethylated Entitylysine 20 was found to be the main methylation product, whereas the monomethyl derivative was present in much smaller amounts.
0.9810	Methylation	In rat kidney and liver the dimethylated Entitylysine 20 was found to be the main methylation product, whereas the Methylationmonomethyl derivative was present in much smaller amounts.

(672)

In addition, for the first time a trimethylated form of lysine 20 of H4 was found in mammalian tissue.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Relation	Text (Head - Tail)
0.9998	Methylation	In addition, for the first time a Methylationtrimethylated form of Entitylysine 20 of H4 was found in mammalian tissue.
0.9992	Methylation	In addition, for the first time a Methylationtrimethylated form of Entitylysine 20 of H4 was found in mammalian tissue.

(673)

A significant increase in this trimethylated histone H4 was detected in organs of animals older than 30 days, whereas the amounts of mono - and dimethylated forms did not essentially change in organs from young ( 10 days old ) or old animals ( 30 and 450 days old ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9995	Methylation	A significant increase in this trimethylated Proteinhistone H4 was detected in organs of animals older than 30 days, whereas the amounts of Methylationmono - and dimethylated forms did not essentially change in organs from young ( 10 days old ) or old animals ( 30 and 450 days old ).
0.9994	Methylation	A significant increase in this Methylationtrimethylated Proteinhistone H4 was detected in organs of animals older than 30 days, whereas the amounts of mono - and dimethylated forms did not essentially change in organs from young ( 10 days old ) or old animals ( 30 and 450 days old ).
0.9991	Methylation	A significant increase in this trimethylated Proteinhistone H4 was detected in organs of animals older than 30 days, whereas the amounts of mono - and Methylationdimethylated forms did not essentially change in organs from young ( 10 days old ) or old animals ( 30 and 450 days old ).

(674)

Trimethylated H4 was also detected in transformed cells ; although it was present in only trace amounts in logarithmically growing cells, we found an increase in trimethylated lysine 20 in cells in the stationary phase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9982	Methylation	MethylationTrimethylated ProteinH4 was also detected in transformed cells ; although it was present in only trace amounts in logarithmically growing cells, we found an increase in trimethylated lysine 20 in cells in the stationary phase.
0.9844	Methylation	Trimethylated H4 was also detected in transformed cells ; although it was present in only trace amounts in logarithmically growing cells, we found an increase in Methylationtrimethylated Entitylysine 20 in cells in the stationary phase.

(675)

In the cystathionine beta - synthase knockout mouse, elevations in total plasma homocysteine increase tissue S - adenosylhomocysteine, but responses of S - adenosylmethionine and DNA methylation are tissue specific.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(676)

The cystathionine beta - synthase knockout mouse provides a unique opportunity to study biochemical consequences of a defective cystathionine beta - synthase enzyme.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(677)

The present study was undertaken to assess the effect of elevated plasma total homocysteine caused by cystathionine beta - synthase deficiency on one - carbon metabolism in 10 homozygous mutant mice and 10 age - and sex - matched wild - type mice.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(678)

Plasma total homocysteine levels, S - adenosylmethionine and S - adenosylhomocysteine concentrations in liver, kidney and brain were measured by HPLC.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(679)

Tissue DNA methylation status was measured by in vitro DNA methyl acceptance.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(680)

Plasma total homocysteine concentration in food - deprived homozygous mutant mice ( 271. 1 + / - 61. 5 micro mol / L ) was markedly higher than in wild - type mice ( 7. 4 + / - 2. 9 micro mol / L ) ( P < 0. 001 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(681)

In liver only, S - adenosylmethionine concentrations were higher in the homozygous mutant mice ( 35. 6 + / - 5. 9 nmol / g ) than in wild type mice ( 19. 1 + / - 6. 1 nmol / g ) ( P < 0. 001 ) and tended to be lower in kidney ( P = 0. 07 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(682)

In contrast, S - adenosylhomocysteine concentrations were significantly higher in homozygous mutant mice compared with wild - type mice in all tissues studied.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(683)

Genomic DNA methylation status in homozygous mutant compared with wild - type mice was lower in liver ( P = 0. 037 ) and tended to be lower in kidney ( P = 0. 077 ) but did not differ in brain ( P = 0. 46 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(684)

The results of this study are consistent with the predicted role of cystathionine beta - synthase in the regulation of plasma total homocysteine levels and tissue S - adenosylhomocysteine levels.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(685)

However, the fact that the absence of the enzyme had differential effects on S - adenosylmethionine concentrations and DNA methylation status in different tissues suggests that regulation of biological methylation is a complex tissue - specific phenomenon.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(686)

Glycosylation of human proteinase - activated receptor - 2 ( hPAR2 ) : role in cell surface expression and signalling.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9997	Methylation	GlycosylationGlycosylation of human Proteinproteinase - activated receptor - 2 ( hPAR2 ) : role in cell surface expression and signalling.

(687)

We have analysed the role of N - linked glycosylation in regulating human proteinase - activated receptor - 2 ( hPAR ( 2 ) ) expression and function.

Precision: 11.11

Recall: 100.00

F1: 20.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	We have analysed the role of GlycosylationN - linked glycosylation in regulating human Proteinproteinase - activated receptor - 2 ( hPAR ( 2 ) ) expression and function.
0.9998	Methylation	We have analysed the role of N - linked Glycosylationglycosylation in regulating human Proteinproteinase - activated receptor - 2 ( hPAR ( 2 ) ) expression and function.
0.9393	Methylation	We have analysed the role of GlycosylationN - linked glycosylation in regulating human Proteinproteinase - activated receptor - 2 ( hPAR ( 2 ) ) expression and function.
0.8599	Methylation	We have analysed the role of GlycosylationN - linked glycosylation Glycosylationglycosylation in regulating human proteinase - activated receptor - 2 ( hPAR ( 2 ) ) expression and function.
0.8415	Methylation	We have analysed the role of GlycosylationN - linked glycosylation Glycosylationglycosylation in regulating human proteinase - activated receptor - 2 ( hPAR ( 2 ) ) expression and function.
0.8291	Methylation	We have analysed the role of GlycosylationN - linked GlycosylationN - linked glycosylation in regulating human proteinase - activated receptor - 2 ( hPAR ( 2 ) ) expression and function.
0.8230	Methylation	We have analysed the role of GlycosylationN - linked Glycosylationglycosylation in regulating human proteinase - activated receptor - 2 ( hPAR ( 2 ) ) expression and function.
0.6961	Methylation	We have analysed the role of GlycosylationN - linked Glycosylationglycosylation in regulating human proteinase - activated receptor - 2 ( hPAR ( 2 ) ) expression and function.
0.6738	Methylation	We have analysed the role of GlycosylationN - linked glycosylation GlycosylationN - linked glycosylation in regulating human proteinase - activated receptor - 2 ( hPAR ( 2 ) ) expression and function.

(688)

Epitope - tagged wild - type hPAR ( 2 ) ( wt - hPAR ( 2 ) ) or hPAR ( 2 ) that lacked glycosylation sequons ( following site - directed mutagenesis ) in either the N - terminus [ hPAR ( 2 ) N30A ( Asn ( 30 ) - - > Ala ) ], extracellular loop 2 [ ECL2 ; hPAR ( 2 ) N222Q ( Asn ( 222 ) - - > Gln ) or hPAR ( 2 ) N222A ( Asn ( 222 ) - - > Ala ) ] or both ( hPAR ( 2 ) N30A, N222A or hPAR ( 2 ) N30A, N222Q ) were expressed in the Chinese - hamster ovary ( CHO ) fibroblast cell line, Pro5.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(689)

Western blot analysis of wt - hPAR ( 2 ) showed mature wt - hPAR ( 2 ) to have a molecular mass of 55 - 100 kDa, and 33 - 48 kDa following N - glycosidase F deglycosylation.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Relation	Text (Head - Tail)
0.9993	Methylation	Western blot analysis of wt - hPAR ( 2 ) showed mature Proteinwt - hPAR ( 2 ) to have a molecular mass of 55 - 100 kDa, and 33 - 48 kDa following N - glycosidase F Catalysisdeglycosylation .
0.9958	Methylation	Western blot analysis of wt - hPAR ( 2 ) showed mature wt - hPAR ( 2 ) to have a molecular mass of 55 - 100 kDa, and 33 - 48 kDa following N Protein- glycosidase F Catalysisdeglycosylation .
0.9956	Methylation	Western blot analysis of wt - hPAR ( 2 ) showed mature wt - hPAR ( 2 ) to have a molecular mass of 55 - 100 kDa, and 33 - 48 kDa following ProteinN - glycosidase F Catalysisdeglycosylation .
	Methylation	Western blot analysis of wt - hPAR ( 2 ) showed mature wt - hPAR ( 2 ) to have a molecular mass of 55 - 100 kDa, and 33 - 48 kDa following ProteinN - glycosidase F Deglycosylationdeglycosylation .
	Methylation	Western blot analysis of wt - hPAR ( 2 ) showed mature Proteinwt - hPAR ( 2 ) to have a molecular mass of 55 - 100 kDa, and 33 - 48 kDa following N - glycosidase F Deglycosylationdeglycosylation .

(690)

FACS analysis and immunocytochemistry of the wt - hPAR ( 2 ) and PAR ( 2 ) mutant cell lines revealed that removal of both glycosylation sequons decreases ( 50 % of wt - hPAR ( 2 ) ) cell surface expression.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(691)

Western blot analysis indicated that both N - linked sites are glycosylated.

Precision: 33.33

Recall: 100.00

F1: 50.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	Western blot analysis indicated that both EntityN - linked sites are Glycosylationglycosylated .
0.9979	Methylation	Western blot analysis indicated that both EntityN - linked sites are Glycosylationglycosylated .
0.4813	Methylation	Western blot analysis indicated that both EntityN - linked sites EntityN - linked sites are glycosylated.

(692)

In functional studies, hPAR ( 2 ) N30A displayed a selective and significant increase in sensitivity towards tryptase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(693)

Interestingly, hPAR ( 2 ) N222A displayed a loss in sensitivity towards all PAR ( 2 ) agonists tested.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(694)

However, further analysis revealed receptor sensitivity to alanine mutations in this domain, as the more conservative substitution hPAR ( 2 ) N222Q displayed no change in response to PAR ( 2 ) agonists. hPAR ( 2 ) N30A, N222Q displayed increased sensitivity towards tryptase, but a loss in sensitivity towards trypsin and the synthetic peptide SLIGRL - NH ( 2 ), although this loss in sensitivity towards trypsin and SLIGRL - NH ( 2 ) was secondary to changes in cell - surface expression.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(695)

Finally, expression of sialic - acid - deficient wt - hPAR ( 2 ) in the CHO Lec2 glycosylation - deficient mutant cell line, showed a 40 kDa loss in molecular mass, in addition to a marked and selective increase in sensitivity towards tryptase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(696)

We conclude that hPAR ( 2 ) N - linked glycosylation and sialylation regulates receptor expression and / or signalling.

Precision: 11.11

Recall: 100.00

F1: 20.00

Score	Relation	Text (Head - Tail)
0.9991	Methylation	We conclude that ProteinhPAR ( 2 ) GlycosylationN - linked glycosylation and sialylation regulates receptor expression and / or signalling.
0.9956	Methylation	We conclude that ProteinhPAR ( 2 ) GlycosylationN - linked glycosylation and sialylation regulates receptor expression and / or signalling.
0.9867	Methylation	We conclude that ProteinhPAR ( 2 ) N - linked Glycosylationglycosylation and sialylation regulates receptor expression and / or signalling.
0.9268	Methylation	We conclude that hPAR ( 2 ) GlycosylationN - linked glycosylation Glycosylationglycosylation and sialylation regulates receptor expression and / or signalling.
0.9242	Methylation	We conclude that hPAR ( 2 ) GlycosylationN - linked Glycosylationglycosylation and sialylation regulates receptor expression and / or signalling.
0.8991	Methylation	We conclude that hPAR ( 2 ) GlycosylationN - linked glycosylation Glycosylationglycosylation and sialylation regulates receptor expression and / or signalling.
0.8932	Methylation	We conclude that hPAR ( 2 ) GlycosylationN - linked GlycosylationN - linked glycosylation and sialylation regulates receptor expression and / or signalling.
0.6459	Methylation	We conclude that hPAR ( 2 ) GlycosylationN - linked Glycosylationglycosylation and sialylation regulates receptor expression and / or signalling.
0.6401	Methylation	We conclude that hPAR ( 2 ) GlycosylationN - linked glycosylation GlycosylationN - linked glycosylation and sialylation regulates receptor expression and / or signalling.

(697)

N - acetyltransferase 2 genotype - related sulfapyridine acetylation and its adverse events.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(698)

Sulfapyridine ( SP ), one of the metabolites of sulfasalazine ( SASP ), is further metabolized into N - acetylsulfapyridine ( AcSP ) by polymorphic N - acetyltransferase 2 ( NAT2 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(699)

NAT2 activity has been diagnosed by phenotyping, that is, evaluating plasma concentrations or urinary excretions of tentatively administered test drugs for dose individualization and avoidance of serious adverse events.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(700)

Herein, we investigated the relationship between NAT2 genotypes and the pharmacokinetics of SP in healthy Japanese subjects, as well as the adverse events of SASP in patients with inflammatory bowel disease ( IBD ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(701)

Eight healthy subjects and 13 IBD patients were classified into three groups by NAT2 genotyping ; the homozygote for the wild - type allele ( Rapid Types ), the compound heterozygote for the wild - type and mutant alleles ( Intermediate Types ), and the homozygote for mutant alleles ( Slow Types ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(702)

A single oral dose of 40 mg / kg SASP was administered to each healthy subject, and plasma and urine samples were taken until 51 and 72 h after administration, respectively.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(703)

Both the SP and AcSP concentrations in each sample were determined by the HPLC method.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(704)

The NAT2 genotypes were well - correlated with the plasma concentrations or urinary excretions of SP and AcSP in 8 healthy subjects, except for one Slow Type.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(705)

In patients with IBD, skin rash was seen in 3 of 6 Rapid Types and 1 of 6 Intermediate Types, consistent with the concept that hypersensitive reactions are independent of serum SP concentrations.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(706)

In contrast, SASP dosing - related acute pancreatitis was found in the Slow Type patient.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(707)

In this case, the NAT2 activity was diagnosed by genotyping in advance, and the medical staff could pay scrupulous attention, resulting in no serious subjective symptoms such as abdominal pain, anorexia or fever.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(708)

Further investigations on the relationship between the NAT2 genotype and adverse events are required, although genotyping appeared to be a promising method to avoid such serious adverse events.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(709)

Histone H3 lysine 4 methylation is mediated by Set1 and promotes maintenance of active chromatin states in fission yeast.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9988	Methylation	Histone H3 lysine 4 methylation is Catalysismediated by ProteinSet1 and promotes maintenance of active chromatin states in fission yeast.
0.9803	Methylation	Histone H3 Entitylysine 4 Methylationmethylation is mediated by Set1 and promotes maintenance of active chromatin states in fission yeast.

(710)

Methylation of histone H3 at lysine 4 ( H3 Lys - 4 ) or lysine 9 ( H3 Lys - 9 ) is known to define active and silent chromosomal domains respectively from fission yeast to humans.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9951	Methylation	MethylationMethylation of histone H3 at Entitylysine 4 ( H3 Lys - 4 ) or lysine 9 ( H3 Lys - 9 ) is known to define active and silent chromosomal domains respectively from fission yeast to humans.
0.9914	Methylation	MethylationMethylation of histone H3 at lysine 4 ( H3 Lys - 4 ) or Entitylysine 9 ( H3 Lys - 9 ) is known to define active and silent chromosomal domains respectively from fission yeast to humans.

(711)

However, in budding yeast, H3 Lys - 4 methylation is also necessary for silent chromatin assembly at telomeres and ribosomal DNA.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9993	Methylation	However, in budding yeast, H3 EntityLys - 4 Methylationmethylation is also necessary for silent chromatin assembly at telomeres and ribosomal DNA.

(712)

Here we demonstrate that deletion of set1, which encodes a protein containing an RNA recognition motif at its amino terminus and a SET domain at the carboxy terminus, abolishes H3 Lys - 4 methylation in fission yeast.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Relation	Text (Head - Tail)
0.9979	Methylation	Here we demonstrate that deletion of set1, which encodes a protein containing an RNA recognition motif at its amino terminus and a SET domain at the carboxy terminus, abolishes H3 EntityLys - 4 Methylationmethylation in fission yeast.
0.9305	Methylation	Here we demonstrate that deletion of set1, which encodes a protein containing an RNA recognition motif at its amino terminus and a SET domain at the carboxy terminus, abolishes EntityH3 Lys - 4 Methylationmethylation in fission yeast.

(713)

Unlike in budding yeast, Set1 - mediated H3 Lys - 4 methylation is not required for heterochromatin assembly at the silent mating - type region and centromeres in fission yeast.

Precision: 33.33

Recall: 50.00

F1: 40.00

Score	Relation	Text (Head - Tail)
0.9759	Methylation	Unlike in budding yeast, Set1 - mediated H3 EntityLys - 4 Methylationmethylation is not required for heterochromatin assembly at the silent mating - type region and centromeres in fission yeast.
0.6041	Methylation	Unlike in budding yeast, CatalysisSet1 - mediated ProteinSet1 - mediated H3 Lys - 4 methylation is not required for heterochromatin assembly at the silent mating - type region and centromeres in fission yeast.
0.5512	Methylation	Unlike in budding yeast, ProteinSet1 - mediated H3 CatalysisSet1 - mediated H3 Lys - 4 methylation is not required for heterochromatin assembly at the silent mating - type region and centromeres in fission yeast.
	Methylation	Unlike in budding yeast, ProteinSet1 - mediated ProteinSet1 - mediated H3 Lys - 4 methylation is not required for heterochromatin assembly at the silent mating - type region and centromeres in fission yeast.

(714)

Our analysis suggests that H3 Lys - 4 methylation is a stable histone modification present throughout the cell cycle, including mitosis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9973	Methylation	Our analysis suggests that H3 EntityLys - 4 Methylationmethylation is a stable histone modification present throughout the cell cycle, including mitosis.

(715)

The loss of H3 Lys - 4 methylation in set1Delta cells is correlated with a decrease in histone H3 acetylation levels, suggesting a mechanistic link between H3 Lys - 4 methylation and acetylation of the H3 tail.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9946	Methylation	The loss of H3 EntityLys - 4 Methylationmethylation in set1Delta cells is correlated with a decrease in histone H3 acetylation levels, suggesting a mechanistic link between H3 Lys - 4 methylation and acetylation of the H3 tail.
0.9913	Methylation	The loss of H3 Lys - 4 methylation in set1Delta cells is correlated with a decrease in histone H3 acetylation levels, suggesting a mechanistic link between H3 EntityLys - 4 Methylationmethylation and acetylation of the H3 tail.
0.9854	Methylation	The loss of H3 Lys - 4 methylation in set1Delta cells is correlated with a decrease in histone H3 acetylation levels, suggesting a mechanistic link between H3 Lys - 4 methylation and Acetylationacetylation of the H3 Entitytail .
0.9851	Methylation	The loss of H3 Lys - 4 methylation in set1Delta cells is correlated with a decrease in Proteinhistone H3 Acetylationacetylation levels, suggesting a mechanistic link between H3 Lys - 4 methylation and acetylation of the H3 tail.

(716)

We suggest that methylation of H3 Lys - 4 primarily acts in the maintenance of transcriptionally poised euchromatic domains, and that this modification is dispensable for heterochromatin formation in fission yeast, which instead utilizes H3 Lys - 9 methylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9987	Methylation	We suggest that methylation of H3 Lys - 4 primarily acts in the maintenance of transcriptionally poised euchromatic domains, and that this modification is dispensable for heterochromatin formation in fission yeast, which instead utilizes H3 EntityLys - 9 Methylationmethylation .
0.9942	Methylation	We suggest that Methylationmethylation of H3 EntityLys - 4 primarily acts in the maintenance of transcriptionally poised euchromatic domains, and that this modification is dispensable for heterochromatin formation in fission yeast, which instead utilizes H3 Lys - 9 methylation.

(717)

Hypoxia - inducible factor asparaginyl hydroxylase ( FIH - 1 ) catalyses hydroxylation at the beta - carbon of asparagine - 803.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(718)

Asparagine - 803 in the C - terminal transactivation domain of human hypoxia - inducible factor ( HIF ) - 1 alpha - subunit is hydroxylated by factor inhibiting HIF - 1 ( FIH - 1 ) under normoxic conditions causing abrogation of the HIF - 1alpha / p300 interaction.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9993	Methylation	Asparagine - 803 in the C - terminal transactivation domain of human hypoxia - inducible factor ( HIF ) - 1 alpha - subunit is Hydroxylationhydroxylated by Proteinfactor inhibiting HIF - 1 ( FIH - 1 ) under normoxic conditions causing abrogation of the HIF - 1alpha / p300 interaction.
0.9951	Methylation	EntityAsparagine - 803 in the C - terminal transactivation domain of human hypoxia - inducible factor ( HIF ) - 1 alpha - subunit is Hydroxylationhydroxylated by factor inhibiting HIF - 1 ( FIH - 1 ) under normoxic conditions causing abrogation of the HIF - 1alpha / p300 interaction.

(719)

NMR and other analyses of a hydroxylated HIF fragment produced in vitro demonstrate that hydroxylation occurs at the beta - carbon of Asn - 803 and imply production of the threo - isomer, in contrast with other known aspartic acid / asparagine hydroxylases that produce the erythro - isomer.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Relation	Text (Head - Tail)
0.9994	Methylation	NMR and other analyses of a hydroxylated HIF fragment produced in vitro demonstrate that Hydroxylationhydroxylation occurs at the beta - carbon of EntityAsn - 803 and imply production of the threo - isomer, in contrast with other known aspartic acid / asparagine hydroxylases that produce the erythro - isomer.
0.5956	Methylation	NMR and other analyses of a Hydroxylationhydroxylated HIF fragment produced in vitro demonstrate that hydroxylation occurs at the beta - carbon of EntityAsn - 803 and imply production of the threo - isomer, in contrast with other known aspartic acid / asparagine hydroxylases that produce the erythro - isomer.

(720)

Effect of the alcohol extract of the seeds of Mucuna pruriens on free radicals and oxidative stress in albino rats.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(721)

In vitro and in vivo studies were made with an alcohol extract of the seeds of Mucuna pruriens ( Fabaceae ) to investigate its antioxidant property.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(722)

In vitro studies were carried out in rat liver homogenate to investigate the chemical interaction of various phytochemicals with different species of free radicals.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(723)

The effect was also checked on iron - induced lipid peroxidation, oxidation of GSH content, and its interaction with hydroxyl and superoxide radicals.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(724)

There was no change on the rate of aerial oxidation of GSH content but it significantly inhibited FeSO ( 4 ) induced lipid peroxidation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(725)

It also inhibited the specific chemical reactions induced by superoxides and hydroxyl radicals.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(726)

The removal of these species was through direct chemical interaction.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(727)

An in vivo study on albino rats for 30 days showed no toxic effect up to a dose of 600 mg / kg body weight, on oral administration.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(728)

There was no change in the level of TBA - reactive substances, reduced glutathione content and SOD activity in the liver.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(729)

The activity of serum GOT, GPT and alkaline phosphatase was also unchanged.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(730)

Thus it could be concluded that the alcohol extract of the seeds of M. pruriens has an antilipid peroxidation property, which is mediated through the removal of superoxides and hydroxyl radicals.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(731)

Induction of apoptosis of human B - CLL and ALL cells by a novel retinoid and its nonretinoidal analog.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(732)

We have recently described a novel retinoid 6 - [ 3 - ( 1 - adamantyl ) - 4 - hydroxyphenyl ] - 2 - naphthalenecarboxylic acid ( CD437 / AHPN ) that induces apoptosis in a number of malignant cell types.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(733)

We now describe our studies examining the effects of CD437 and a nonretinoidal analog ( MM002 ) on the in vitro proliferation of the ALL - REH cell line, the in vitro and in vivo growth of a novel Epstein - Barr virus - negative ( EBV ( - ) ) B - cell chronic lymphocytic leukemia ( B - CLL ) cell line ( WSU - CLL ), and primary cultures of human B - CLL and acute lymphoblastic leukemia ( ALL ) cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(734)

CD437 and MM002 induce apoptosis in both cell lines, as indicated by the activation of caspase - 2 and caspase - 3, cleavage of poly ( adenosine diphosphate - ribose ) ( poly ( ADP - ribose ) ) polymerase, increase in annexin V binding, and subsequent nuclear fragmentation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(735)

CD437 - mediated apoptosis was not associated with the modulation of Bcl - 2, Bax, or Mcl - 1 levels, but was associated with the cleavage of the antiapoptotic protein Bcl - X ( L ) to a proapoptotic 18 - kD form.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(736)

This cleavage of Bcl - X ( L ) was dependent on caspase - 3 activation since Bcl - X ( L ) cleavage and apoptosis were inhibited by the caspase - 3 inhibitor Z - DVED - fmk.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(737)

CD437 markedly inhibited the growth of WSU - CLL cells in severe combined immunodeficiency ( SCID ) mice.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(738)

Tumor growth inhibition, growth delay, and log cell kill were 85. 7 %, 21 days, and 2. 1, respectively, in the treated mice.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(739)

Moreover, 1 of the 5 treated mice was tumor - free longer than 150 days and thus was considered cured.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(740)

Exposure of primary cultures of both B - CLL and ALL cells obtained from patients to CD437 and MM002 resulted in their apoptosis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(741)

These results suggest that CD437 and MM002 analogs may have a potential role in the treatment of B - CLL and ALL.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(742)

Biochemical purification and pharmacological inhibition of a mammalian prolyl hydroxylase acting on hypoxia - inducible factor.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(743)

The product of the von Hippel - Lindau gene, pVHL, targets the alpha subunits of the heterodimeric transcription factor hypoxia - inducible factor ( HIF ) for polyubiquitination in the presence of oxygen.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(744)

The binding of pVHL to HIF is governed by the enzymatic hydroxylation of conserved prolyl residues within peptidic motifs present in the HIFalpha family members.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(745)

By using a biochemical purification strategy, we have identified a human homolog of Caenorhabditis elegans Egl9 as a HIF prolyl hydroxylase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(746)

In addition, we studied the activity of a structurally diverse collection of low molecular weight inhibitors of procollagen prolyl 4 - hydroxylase as potential inhibitors of the HIF hydroxylase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(747)

A model compound of this series stabilized HIF in a variety of cells, leading to the increased production of its downstream target, vascular endothelial growth factor.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(748)

Control of CBP co - activating activity by arginine methylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9998	Methylation	Control of CBP co - activating activity by Entityarginine Methylationmethylation .

(749)

The histone acetyltransferases CREB binding protein ( CBP ) and the related p300 protein function as key transcriptional co - activators in multiple pathways.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(750)

In the case of transcriptional activation by nuclear receptors, ligand promotes the recruitment of co - activators of the p160 family, such as GRIP - 1.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(751)

Subsequently, the p160 co - activators recruit other co - activators via two activation domains, AD1 and AD2.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(752)

AD1 binds CBP or p300, whereas AD2 has been shown to activate transcription through the recruitment of the arginine methyltransferase CARM1.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(753)

Recently, the KIX domain of CBP has been shown to be methylated by CARM1 in vitro.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	Recently, the KIX domain of CBP has been shown to be Catalysismethylated by ProteinCARM1 in vitro.
0.9985	Methylation	Recently, the EntityKIX domain of CBP has been shown to be Catalysismethylated by CARM1 in vitro.
0.9883	Methylation	Recently, the EntityKIX domain of CBP has been shown to be Catalysismethylated by CARM1 in vitro.
	Methylation	Recently, the EntityKIX domain of CBP has been shown to be Methylationmethylated by CARM1 in vitro.
	Methylation	Recently, the KIX domain of CBP has been shown to be Methylationmethylated by ProteinCARM1 in vitro.

(754)

Here, we report that another domain of CBP is specifically methylated by CARM1 on conserved arginine residues in vitro and in vivo.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	Here, we report that another domain of CBP is specifically Catalysismethylated by ProteinCARM1 on conserved arginine residues in vitro and in vivo.
1.0000	Methylation	Here, we report that another domain of ProteinCBP is specifically Catalysismethylated by CARM1 on conserved arginine residues in vitro and in vivo.
	Methylation	Here, we report that another domain of CBP is specifically Methylationmethylated by ProteinCARM1 on conserved arginine residues in vitro and in vivo.
	Methylation	Here, we report that another domain of ProteinCBP is specifically Methylationmethylated by CARM1 on conserved arginine residues in vitro and in vivo.

(755)

We also provide functional evidence that arginine residues methylated by CARM1 play a critical role in GRIP - 1 - dependent transcriptional activation and in hormone - induced gene activation.

Precision: 66.67

Recall: 100.00

F1: 80.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	We also provide functional evidence that arginine residues Methylationmethylated by ProteinCARM1 play a critical role in GRIP - 1 - dependent transcriptional activation and in hormone - induced gene activation.
0.9997	Methylation	We also provide functional evidence that Entityarginine residues Methylationmethylated by CARM1 play a critical role in GRIP - 1 - dependent transcriptional activation and in hormone - induced gene activation.
0.9928	Methylation	We also provide functional evidence that Entityarginine residues Methylationmethylated by CARM1 play a critical role in GRIP - 1 - dependent transcriptional activation and in hormone - induced gene activation.

(756)

Altogether, our data provide strong evidence that arginine methylation represents an important mechanism for modulating co - activator transcriptional activity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(757)

The hepatitis C virus RNA - dependent RNA polymerase membrane insertion sequence is a transmembrane segment.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(758)

The hepatitis C virus ( HCV ) RNA - dependent RNA polymerase ( RdRp ) belongs to a class of membrane proteins termed tail - anchored proteins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(759)

Here, we show that the HCV RdRp C - terminal membrane insertion sequence traverses the phospholipid bilayer as a transmembrane segment.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(760)

Moreover, the HCV RdRp was found to be retained in the endoplasmic reticulum ( ER ) or an ER - derived modified compartment both following transient transfection and in the context of a subgenomic replicon.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(761)

An absolutely conserved GVG motif was not essential for membrane insertion but possibly provides a docking site for transmembrane protein - protein interactions.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(762)

These findings have important implications for the functional architecture of the HCV replication complex.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(763)

Molecular and biochemical characterisation of a novel sulphatase gene : Arylsulfatase G ( ARSG ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(764)

Molecular analysis has provided important insights into the biochemistry and genetics of the sulphatase family of enzymes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(765)

Through bioinformatic searches of the EST database, we have identified a novel gene consisting of 11 exons and encoding a 525 aa protein that shares a high degree of sequence similarity with all sulphatases and in particular with arylsulphatases, hence the tentative name Arylsulfatase G ( ARSG ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(766)

The highest homology is shared with Arylsulfatase A, a lysosomal sulphatase which is mutated in metachromatic leukodistrophy, particularly in the amino - terminal region.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(767)

The 10 amino acids that form the catalytic site are strongly conserved.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(768)

The murine homologue of Arylsulfatase G gene product shows 87 % identity with the human protein.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(769)

To test the function of this novel gene we transfected the full - length cDNA in Cos7 cells, and detected an Arylsulfatase G precursor protein of 62 kDa.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(770)

After glycosylation the precursor is maturated in a 70 kDa form, which localises to the endoplasmic reticulum.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(771)

Northern blot analysis of Arylsulfatase G revealed a ubiquitous expression pattern.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(772)

We tested the sulphatase activity towards two different artificial substrates 4 - methylumbelliferyl ( 4 - MU ) sulphate and p - nitrocatechol sulphate, but no arylsulphatase activity was detectable.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(773)

Further studies are needed to characterise the function of Arylsulfatase G, possibly revealing a novel metabolic pathway.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(774)

Gene - specific targeting of H3K9 methylation is sufficient for initiating repression in vivo.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9990	Methylation	Gene - specific targeting of EntityH3K9 Methylationmethylation is sufficient for initiating repression in vivo.

(775)

Covalent modifications of chromatin have emerged as key determinants of the genome's transcriptional competence.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(776)

Histone H3 lysine 9 ( H3K9 ) methylation is an epigenetic signal that is recognized by HP1 and correlates with gene silencing in a variety of organisms.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9982	Methylation	Histone H3 Entitylysine 9 ( H3K9 ) Methylationmethylation is an epigenetic signal that is recognized by HP1 and correlates with gene silencing in a variety of organisms.

(777)

Discovery of the enzymes that catalyze H3K9 methylation has identified a second gene - specific function for this modification in transcriptional repression.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9989	Methylation	Discovery of the enzymes that catalyze EntityH3K9 Methylationmethylation has identified a second gene - specific function for this modification in transcriptional repression.

(778)

Whether H3K9 methylation is causative in the initiation and establishment of gene repression or is a byproduct of the process leading to the repressed state remains unknown.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9991	Methylation	Whether EntityH3K9 Methylationmethylation is causative in the initiation and establishment of gene repression or is a byproduct of the process leading to the repressed state remains unknown.

(779)

To investigate the role of HMTs and specifically H3K9 methylation in gene repression, we have employed engineered zinc - finger transcription factors ( ZFPs ) to target HMT activity to a specific endogenous gene.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9996	Methylation	To investigate the role of HMTs and specifically EntityH3K9 Methylationmethylation in gene repression, we have employed engineered zinc - finger transcription factors ( ZFPs ) to target HMT activity to a specific endogenous gene.

(780)

By utilizing ZFPs that recognize the promoter of the endogenous VEGF - A gene, and thus employing this chromosomal locus as an in vivo reporter, we show that ZFPs linked to a minimal catalytic HMT domain affect local methylation of histone H3K9 and the consequent repression of target gene expression.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(781)

Furthermore, amino acid substitutions within the HMT that ablate its catalytic activity effectively eliminate the ability of the ZFP fusions to repress transcription.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(782)

Thus, H3K9 methylation is a primary signal that is sufficient for initiating a gene repression pathway in vivo.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9991	Methylation	Thus, EntityH3K9 Methylationmethylation is a primary signal that is sufficient for initiating a gene repression pathway in vivo.

(783)

Studies on the inhibition of human cytochromes P450 by selenocysteine Se - conjugates.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(784)

1. To investigate whether cytochrome P450 ( P450 ) inhibition can contribute to the chemopreventive activity of selenocysteine Se - conjugates ( SeCys conjugates ), 21 SeCys conjugates were screened for their inhibitory potency towards seven of the most important human P450s. 2.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(785)

The majority of the SeCys conjugates produced near complete inhibition of CYP1A1 at a concentration of 250 microm.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(786)

The most potent inhibitor, Se - benzyl - L - selenocysteine, displayed an IC50 of 12. 8 + / - 1. 2 microm.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(787)

CYP2C9, - 2C19 and - 2D6 were moderately ( 50 - 60 % ) inhibited by the SeCys conjugates.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(788)

CYP1A2, - 2E1 and - 3A4 were least inhibited. 3.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(789)

Studies on the susceptibility of CYP1A1 to SeCys conjugates implicated a thiol - reactive intermediate, as evidenced by reduced inhibition levels in the presence of glutathione and N - acetyl cysteine.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(790)

Uncoupling of the P450 - catalytic cycle was of no importance as ROS scavengers did not influence inhibition levels. 4.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(791)

P450 inhibition by two physiologically relevant metabolite classes of SeCys conjugates was also studied.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(792)

N - acetylation of SeCys conjugates consistently increased the inhibitory potency towards CYP1A2, - 2C19, - 2E1 and - 3A4.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(793)

Beta - lyase catalysed bioactivation of alkyl - substituted SeCys conjugates or Se - benzyl - L - selenocysteine produced little or no additional inhibition of P450 activity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(794)

For Se - phenyl - L - selenocysteine, however, significant increases in P450 inhibition were obtained by beta - lyase pre - incubation. 5.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(795)

It is concluded that the potent and relatively selective CYP1A1 inhibition exerted by SeCys conjugates may contribute to their chemopreventive activity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(796)

Identification of a lactoferrin - derived peptide possessing binding activity to hepatitis C virus E2 envelope protein.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(797)

Bovine and human lactoferrins ( LF ) prevent hepatitis C virus ( HCV ) infection in cultured human hepatocytes ; the preventive mechanism is thought to be the direct interaction between LF and HCV.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(798)

To clarify this hypothesis, we have characterized the binding activity of LF to HCV E2 envelope protein and have endeavored to determine which region ( s ) of LF are important for this binding activity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(799)

Several regions of human LF have been expressed and purified as thioredoxin - fused proteins in Escherichia coli.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(800)

Far - Western blot analysis using these LF fragments and the E2 protein, expressed in Chinese hamster ovary cells, revealed that the 93 carboxyl amino acids of LF specifically bound to the E2 protein.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(801)

The 93 carboxyl amino acids of LFs derived from bovine and horse cells also possessed similar binding activity to the E2 protein.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(802)

In addition, the amino acid sequences of these carboxyl regions appeared to show partial homology to CD81, a candidate receptor for HCV, and the binding activity of these carboxyl regions was also comparable with that of CD81.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(803)

Further deletion analysis identified 33 amino acid residues as the minimum binding site in the carboxyl region of LF, and the binding specificity of these 33 amino acids was also confirmed by using 33 maltose - binding protein - fused amino acids.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(804)

Furthermore, we demonstrated that the 33 maltose - binding protein - fused amino acids prevented HCV infection in cultured human hepatocytes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(805)

In addition, the site - directed mutagenesis to an Ala residue in both terminal residues of the 33 amino acids revealed that Cys at amino acid 628 was determined to be critical for binding to the E2 protein.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(806)

These results led us to consider the development of an effective anti - HCV peptide.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(807)

This is the first identification of a natural protein - derived peptide that specifically binds to HCV E2 protein and prevents HCV infection.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(808)

Glucocorticoid suppression of nuclear factor - kappa B : a role for histone modifications.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(809)

Corticosteroids are by far the most effective treatment for chronic inflammatory diseases such as asthma.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(810)

Inflammation in asthma is characterized by the increased expression of multiple inflammatory genes, including those that encode cytokines, chemokines, adhesion molecules, and inflammatory enzymes and receptors.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(811)

Increased expression of inflammatory genes is regulated by pro - inflammatory transcription factors, such as nuclear factor kappa B ( NF - kappa B ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(812)

These bind to, and activate, co - activator molecules that then acetylate core histones resulting in elevated gene transcription.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	These bind to, and activate, co - activator molecules that then Acetylationacetylate core Proteinhistones resulting in elevated gene transcription.

(813)

Corticosteroids reverse histone acetylation at the site of inflammatory gene transcription, either by direct binding of the activated glucocorticoid receptor to NF - kappa B - associated co - activators or by recruitment of histone deacetylases to the activated transcription complex.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9992	Methylation	Corticosteroids reverse Proteinhistone Acetylationacetylation at the site of inflammatory gene transcription, either by direct binding of the activated glucocorticoid receptor to NF - kappa B - associated co - activators or by recruitment of histone deacetylases to the activated transcription complex.

(814)

Understanding how corticosteroids work in asthma may help in designing novel corticosteroids with fewer systemic effects, as well as novel anti - inflammatory approaches.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(815)

Probing the conformational and dynamical effects of O - glycosylation within the immunodominant region of a MUC1 peptide tumor antigen.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	Probing the conformational and dynamical effects of GlycosylationO - glycosylation within the Entityimmunodominant region of a MUC1 peptide tumor antigen.

(816)

MUC1 mucin is a large transmembrane glycoprotein, the extracellular domain of which is formed by a repeating 20 amino acid sequence, GVTSAPDTRPAPGSTAPPAH.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(817)

In normal breast epithelial cells, the extracellular domain is densely covered with highly branched complex carbohydrate structures.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(818)

However, in neoplastic breast tissue, the extracellular domain is under - glycosylated, resulting in the exposure of a highly immunogenic core peptide epitope ( PDTRP in bold above ), as well as in the exposure of normally cryptic core Tn ( GalNAc ), STn ( sialyl alpha2 - 6 GalNAc ) and TF ( Gal beta1 - 3 GalNAc ) carbohydrates.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Relation	Text (Head - Tail)
0.9952	Methylation	However, in neoplastic breast tissue, the Glycosylationextracellular Entitydomain is under - glycosylated, resulting in the exposure of a highly immunogenic core peptide epitope ( PDTRP in bold above ), as well as in the exposure of normally cryptic core Tn ( GalNAc ), STn ( sialyl alpha2 - 6 GalNAc ) and TF ( Gal beta1 - 3 GalNAc ) carbohydrates.

(819)

Here, we report the results of 1H NMR structural studies, natural abundance 13C NMR relaxation measurements and distance - restrained MD simulations designed to probe the structural and dynamical effects of Tn - glycosylation within the PDTRP core peptide epitope.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(820)

Two synthetic peptides were studied : a nine - residue MUC1 peptide of the sequence, Thr1 - Ser2 - Ala3 - Pro4 - Asp5 - Thr6 - Arg7 - Pro8 - Ala9, and a Tn - glycosylated version of this peptide, Thr1 - Ser2 - Ala3 - Pro4 - Asp5 - Thr6 ( alphaGalNAc ) - Arg7 - Pro8 - Ala9.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(821)

The results of these studies show that a type I beta - turn conformation is adopted by residues PDTR within the PDTRP region of the unglycosylated MUC1 sequence.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9996	Methylation	The results of these studies show that a type I beta - turn conformation is adopted by residues PDTR within the PDTRP region of the Glycosylationunglycosylated ProteinMUC1 sequence.

(822)

The existence of a similar beta - turn within the PDTRP core peptide epitope of the under - glycosylated cancer - associated MUC1 mucin protein might explain the immunodominance of this region in vivo, as the presence of defined secondary structure within peptide epitope regions has been correlated with increased immunogenicity in other systems.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	The existence of a similar beta - turn within the PDTRP core peptide Entityepitope of the Glycosylationunder - glycosylated cancer - associated MUC1 mucin protein might explain the immunodominance of this region in vivo, as the presence of defined secondary structure within peptide epitope regions has been correlated with increased immunogenicity in other systems.
	Methylation	The existence of a similar beta - turn within the EntityPDTRP core peptide epitope of the Glycosylationunder - glycosylated cancer - associated MUC1 mucin protein might explain the immunodominance of this region in vivo, as the presence of defined secondary structure within peptide epitope regions has been correlated with increased immunogenicity in other systems.

(823)

Our results have also shown that Tn glycosylation at the central threonine within the PDTRP core epitope region shifts the conformational equilibrium away from the type I beta - turn conformation and toward a more rigid and extended state.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(824)

The significance of these results are discussed in relation to the possible roles that peptide epitope secondary structure and glycosylation state may play in MUC1 tumor immunogenicity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	The significance of these results are discussed in relation to the possible roles that peptide epitope secondary structure and Glycosylationglycosylation state may play in ProteinMUC1 tumor immunogenicity.

(825)

Lysine - 79 of histone H3 is hypomethylated at silenced loci in yeast and mammalian cells : a potential mechanism for position - effect variegation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9968	Methylation	EntityLysine - 79 of histone H3 is Methylationhypomethylated at silenced loci in yeast and mammalian cells : a potential mechanism for position - effect variegation.

(826)

Methylation of lysine - 79 ( K79 ) within the globular domain of histone H3 by Dot1 methylase is important for transcriptional silencing and for association of the Sir silencing proteins in yeast.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9994	Methylation	MethylationMethylation of Entitylysine - 79 ( K79 ) within the globular domain of histone H3 by Dot1 methylase is important for transcriptional silencing and for association of the Sir silencing proteins in yeast.
0.9926	Methylation	MethylationMethylation of lysine - 79 ( K79 ) within the globular domain of histone H3 by ProteinDot1 methylase is important for transcriptional silencing and for association of the Sir silencing proteins in yeast.

(827)

Here, we show that the level of H3 - K79 methylation is low at all Sir - dependent silenced loci but not at other transcriptionally repressed regions.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Relation	Text (Head - Tail)
0.9991	Methylation	Here, we show that the level of EntityH3 - K79 Methylationmethylation is low at all Sir - dependent silenced loci but not at other transcriptionally repressed regions.
	Methylation	Here, we show that the level of ProteinH3 - K79 Methylationmethylation is low at all Sir - dependent silenced loci but not at other transcriptionally repressed regions.

(828)

Hypomethylation of H3 - K79 at the telomeric and silent mating - type loci, but not the ribosomal DNA, requires the Sir proteins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	MethylationHypomethylation of ProteinH3 - K79 at the telomeric and silent mating - type loci, but not the ribosomal DNA, requires the Sir proteins.

(829)

Overexpression of Sir3 concomitantly extends the domain of Sir protein association and H3 - K79 hypomethylation at telomeres.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Relation	Text (Head - Tail)
0.9994	Methylation	Overexpression of Sir3 concomitantly extends the domain of Sir protein association and EntityH3 - K79 Methylationhypomethylation at telomeres.
	Methylation	Overexpression of Sir3 concomitantly extends the domain of Sir protein association and ProteinH3 - K79 Methylationhypomethylation at telomeres.

(830)

In mammalian cells, H3 - K79 methylation is found at loci that are active for V ( D ) J recombination, but not at recombinationally inactive loci that are heterochromatic.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Relation	Text (Head - Tail)
0.9996	Methylation	In mammalian cells, EntityH3 - K79 Methylationmethylation is found at loci that are active for V ( D ) J recombination, but not at recombinationally inactive loci that are heterochromatic.
	Methylation	In mammalian cells, ProteinH3 - K79 Methylationmethylation is found at loci that are active for V ( D ) J recombination, but not at recombinationally inactive loci that are heterochromatic.

(831)

These results suggest that H3 - K79 methylation is an evolutionarily conserved marker of active chromatin regions, and that silencing proteins block the ability of Dot1 to methylate histone H3.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9989	Methylation	These results suggest that H3 - K79 methylation is an evolutionarily conserved marker of active chromatin regions, and that silencing proteins block the ability of ProteinDot1 to Methylationmethylate histone H3.
0.9968	Methylation	These results suggest that ProteinH3 - K79 Methylationmethylation is an evolutionarily conserved marker of active chromatin regions, and that silencing proteins block the ability of Dot1 to methylate histone H3.
0.9967	Methylation	These results suggest that H3 - K79 methylation is an evolutionarily conserved marker of active chromatin regions, and that silencing proteins block the ability of Dot1 to Methylationmethylate Proteinhistone H3 .

(832)

Further, they suggest that Sir proteins preferentially bind chromatin with hypomethylated H3 - K79 and then block H3 - K79 methylation.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Relation	Text (Head - Tail)
0.9969	Methylation	Further, they suggest that Sir proteins preferentially bind chromatin with hypomethylated H3 - K79 and then block EntityH3 - K79 Methylationmethylation .
0.9873	Methylation	Further, they suggest that Sir proteins preferentially bind chromatin with Methylationhypomethylated EntityH3 - K79 and then block H3 - K79 methylation.
	Methylation	Further, they suggest that Sir proteins preferentially bind chromatin with hypomethylated H3 - K79 and then block ProteinH3 - K79 Methylationmethylation .
	Methylation	Further, they suggest that Sir proteins preferentially bind chromatin with Methylationhypomethylated ProteinH3 - K79 and then block H3 - K79 methylation.

(833)

This positive feedback loop, and the reverse loop in which H3 - K79 methylation weakens Sir protein association and leads to further methylation, suggests a model for position - effect variegation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9995	Methylation	This positive feedback loop, and the reverse loop in which ProteinH3 - K79 Methylationmethylation weakens Sir protein association and leads to further methylation, suggests a model for position - effect variegation.

(834)

Cyclosporin A prevents the hypoxic adaptation by activating hypoxia - inducible factor - 1alpha Pro - 564 hydroxylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9987	Methylation	Cyclosporin A prevents the hypoxic adaptation by activating hypoxia - inducible factor - 1alpha EntityPro - 564 Hydroxylationhydroxylation .

(835)

The mechanism by which hypoxia induces gene transcription involves the inhibition of hypoxia - inducible factor ( HIF ) - 1alpha prolyl hydroxylase activity, which prevents von Hippel - Lindau ( vHL ) - dependent targeting of HIF - 1alpha to the ubiquitin - proteasome pathway.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(836)

HIF - 1alpha is stabilized, translocates to the nucleus, interacts with hypoxia - responsive elements, and promotes the activation of target genes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(837)

This report shows that cyclosporin A ( CsA ) interferes with the hypoxic signaling cascade in C6 glioma cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(838)

CsA inhibits hypoxia - dependent gene transcription in a reporter gene assay and prevents the hypoxic accumulation of HIF - 1alpha.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(839)

Addition of the 530 - 603 C - terminal oxygen - dependent degradation ( ODD ) domain of HIF - 1alpha to the green fluorescent protein ( GFP ) destabilized the protein in an oxygen - dependent manner.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(840)

CsA prevented the hypoxic stabilization of an ODD. GFP fusion protein.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(841)

An assay for 2 - oxoglutarate - dependent dioxygenases was developed using a light mitochondrial kidney fraction as a source of enzyme.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(842)

It uses the capacity of specific peptides to stimulate the degradation of [ ( 14 ) C ] 2 - oxoglutarate.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(843)

CsA stimulated the enzymatic activity in the presence of a peptide that mimicked the 557 - 576 sequence of HIF - 1alpha.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(844)

The enzyme promoted [ ( 35 ) S ] vHL binding to glutathione S - transferase ( GST ). ODD fusion protein.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(845)

This association increased in the presence of CsA.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(846)

CsA effects were not observed when the proline residue corresponding to Pro - 564 in the HIF - 1alpha sequence was replaced by a hydroxyproline or an alanine residue.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(847)

Finally, CsA increased vHL - ODD interaction during hypoxia.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(848)

We conclude that CsA destabilizes HIF - 1alpha by promoting hydroxylation of Pro - 564 in the ODD domain.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	We conclude that CsA destabilizes HIF - 1alpha by promoting Hydroxylationhydroxylation of EntityPro - 564 in the ODD domain.

(849)

Such a mechanism may prevent hypoxic adaptation during CsA - induced nephrotoxicity and contribute to the adverse effects of this drug.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(850)

A role for N - glycanase in the cytosolic turnover of glycoproteins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(851)

Successful maturation determines the intracellular fate of secretory and membrane proteins in the endoplasmic reticulum ( ER ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(852)

Failure of proteins to fold or assemble properly can lead to their retention in the ER and redirects them to the cytosol for degradation by the proteasome.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(853)

Proteasome inhibitors can yield deglycosylated cytoplasmic intermediates that are the result of an N - glycanase activity, believed to act prior to destruction of these substrates by the proteasome.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(854)

A gene encoding a yeast peptide : N - glycanase, PNG1, has been cloned, but this N - glycanase and its mammalian homolog were reported to be incapable of deglycosylating full - length glycoproteins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(855)

We show that both the yeast PNG1 enzyme and its mammalian homolog display N - glycanase activity towards intact glycoproteins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(856)

As substrates, cytosolic PNGase activity prefers proteins containing high - mannose over those bearing complex type oligosaccharides.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(857)

Importantly, PNG1 discriminates between non - native and folded glycoproteins, consistent with a role for N - glycanase in cytoplasmic turnover of glycoproteins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(858)

Preclinical evaluation of antineoplastic activity of inhibitors of DNA methylation ( 5 - aza - 2'- deoxycytidine ) and histone deacetylation ( trichostatin A, depsipeptide ) in combination against myeloid leukemic cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9998	Methylation	Preclinical evaluation of antineoplastic activity of inhibitors of DNA methylation ( 5 - aza - 2'- deoxycytidine ) and Proteinhistone Deacetylationdeacetylation ( trichostatin A, depsipeptide ) in combination against myeloid leukemic cells.

(859)

During the development of leukemia, genes that suppress growth and induce differentiation can be silenced by aberrant DNA methylation and by changes in chromatin structure that involve histone deacetylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	During the development of leukemia, genes that suppress growth and induce differentiation can be silenced by aberrant DNA methylation and by changes in chromatin structure that involve Proteinhistone Deacetylationdeacetylation .

(860)

It has been reported that a positive interaction between DNA methylation and histone deacetylation takes place to inhibit transcription.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9998	Methylation	It has been reported that a positive interaction between DNA methylation and Proteinhistone Deacetylationdeacetylation takes place to inhibit transcription.

(861)

Based on this observation, our working hypothesis was that a combination of inhibitors of these processes should produce an enhancement of their antineoplastic activity on leukemic cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(862)

The cytosine nucleoside analog, 5 - aza - 2'- deoxycytidine ( 5AZA ), is a potent inhibitor of DNA methylation, which can activate tumor suppressor genes in leukemic cells that have been silenced by aberrant methylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(863)

In clinical trials, 5AZA was demonstrated to be an active antileukemic agent.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(864)

Histone deacetylase inhibitors ( HDI ) can also activate gene expression in leukemic cell lines by producing changes in chromatin configuration, and show antineoplastic activity in preclinical studies.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(865)

In this report, we investigated the in vitro antineoplastic activity of 5AZA, alone and in combination with the HDI, trichostatin A ( TSA ) and depsipeptide ( FR901228, depsi ), on the human myeloid leukemic cell lines, HL - 60 and KG1a.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(866)

The results showed that the combination of 5AZA with TSA or depsi produced a greater inhibition of growth and DNA synthesis and a greater loss of clonogenicity than either agent alone.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(867)

These results suggest that 5AZA used in combination with HDI may be an interesting chemotherapeutic regimen to investigate in patients with acute myeloid leukemia that is resistant to conventional chemotherapy.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(868)

Complex - type biantennary N - glycans of recombinant human transferrin from Trichoplusia ni insect cells expressing mammalian [ beta ] - 1, 4 - galactosyltransferase and [ beta ] - 1, 2 - N - acetylglucosaminyltransferase II.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(869)

A novel recombinant baculovirus expression vector was used to produce His - tagged human transferrin in a transformed insect cell line ( Tn5beta4GalT ) that constitutively expresses a mammalian beta - 1, 4 - galactosyltransferase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(870)

This virus encoded the His - tagged human transferrin protein in conventional fashion under the control of the very late polyhedrin promoter.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(871)

In addition, to enhance the synthesis of galactosylated biantennary N - glycans, this virus encoded human beta - 1, 2 - N - acetylglucosaminyltransferase II under the control of an immediate - early ( ie1 ) promoter.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(872)

Detailed analyses by MALDI - TOF MS, exoglycosidase digestion, and two - dimensional HPLC revealed that the N - glycans on the purified recombinant human transferrin produced by this virus - host system included four different fully galactosylated, biantennary, complex - type glycans.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(873)

Thus, this study describes a novel baculovirus - host system, which can be used to produce a recombinant glycoprotein with fully galactosylated, biantennary N - glycans.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(874)

Overexpression of the CT GalNAc transferase inhibits muscular dystrophy in a cleavage - resistant dystroglycan mutant mouse.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(875)

Transgenic mice that express dystroglycan containing a serine to alanine point mutation at the normal site of cleavage ( DG ( S654A ) ) in their skeletal muscles fail to express endogenously cleaved dystroglycan and have muscular dystrophy [ Neuromusc.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(876)

Disord., in press ].

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(877)

Dystrophic DG ( S654A ) muscles have reduced binding of antibodies, including VIA4 - 1, that recognize carbohydrate antigens on alpha dystroglycan, a finding similar to muscles in some forms of congenital muscular dystrophy.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(878)

Here we describe one DG ( S654A ) transgenic line where VIA4 - 1 antibody binding is absent in skeletal muscle.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(879)

In theory, the absence of this carbohydrate antigen should inhibit later glycosylation events that would occur on the structure or structures this antibody binds to.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(880)

One such modification is likely to be the CT carbohydrate antigen, which is present on alpha dystroglycan in muscles overexpressing the CT GalNAc transferase [ Dev.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(881)

Biol. 242 ( 2002 ) 58 ].

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(882)

To test the relationship between the VIA4 - 1 and CT carbohydrate antigens, we made DG ( S654A ) / CT GalNAc transferase ( DG ( S654A ) / CT ) transgenic mice.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(883)

Surprisingly, dystroglycan was cleaved, and alpha dystroglycan was glycosylated with the VIA4 - 1 antigen, in DG ( S654A ) / CT muscles.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Relation	Text (Head - Tail)
1.0000	Methylation	Surprisingly, dystroglycan was cleaved, and Proteinalpha dystroglycan was Glycosylationglycosylated with the VIA4 - 1 antigen, in DG ( S654A ) / CT muscles.
1.0000	Methylation	Surprisingly, dystroglycan was cleaved, and alpha Proteindystroglycan was Glycosylationglycosylated with the VIA4 - 1 antigen, in DG ( S654A ) / CT muscles.

(884)

In addition, muscles in DG ( S654A ) / CT transgenic mice had little or no evidence of muscular dystrophy when compared to DG ( S654A ) littermates.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(885)

These experiments demonstrate that the CT GalNAc transferase can affect the post - translational processing of dystroglycan and the extent of muscular dystrophy even in muscles where the VIA4 - 1 antigen is not present.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(886)

Characterization of two novel proteins, NgRH1 and NgRH2, structurally and biochemically homologous to the Nogo - 66 receptor.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(887)

Nogo - 66 receptor ( NgR ) has recently been identified as the neuronal receptor of the myelin - associated proteins Nogo - A, oligodendrocyte protein ( OMgp ) and myelin - associated glycoprotein ( MAG ), and mediates inhibition of axonal regeneration both in vitro and in vivo.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(888)

Through database searches, we have identified two novel proteins ( NgRH1 and NgRH2 ) that turned out to be homologous in their primary structures, biochemical properties and expression patterns to NgR.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(889)

Like NgR, the homologues contain eight leucine - rich repeats ( LRR ) flanked by a leucine - rich repeat C - terminus ( LRRCT ) and a leucine - rich repeat N - terminus ( LRRNT ), and also have a C - terminal GPI signal sequence.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(890)

Northern blot analysis showed predominant expression of NgRH1 and NgRH2 mRNA in the brain.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(891)

In situ hybridization and immunohistochemistry on rat brain slices revealed neuronal expression of the genes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(892)

NgRH1 and NgRH2 were detected on the cell surface of recombinant cell lines as N - glycosylated GPI anchored proteins and, consistent with other GPI anchored proteins, were localized within the lipid rafts of cellular membranes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9998	Methylation	NgRH1 and ProteinNgRH2 were detected on the cell surface of recombinant cell lines as GlycosylationN - glycosylated GPI anchored proteins and, consistent with other GPI anchored proteins, were localized within the lipid rafts of cellular membranes.
0.9995	Methylation	ProteinNgRH1 and NgRH2 were detected on the cell surface of recombinant cell lines as GlycosylationN - glycosylated GPI anchored proteins and, consistent with other GPI anchored proteins, were localized within the lipid rafts of cellular membranes.

(893)

In addition, an N - terminal proteolytic fragment of NgR comprising the majority of the ectodomain was found to be constitutively secreted from cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(894)

Our data indicate that NgR, NgRH1 and NgRH2 constitute a novel receptor protein family, which may play related roles within the CNS.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(895)

Synthesis of hydroxymethylglutathione from glutathione and L - serine catalyzed by carboxypeptidase Y.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(896)

Hydroxymethylglutathione ( gamma - L - glutamyl - L - cysteinyl - L - serine ; hmGSH ) occurs in many species belonging to the family Gramineae, but the biosynthetic pathway for hmGSH has not been identified.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(897)

We found that carboxypeptidase Y ( CPY ), but not carboxypeptidase A, catalyzed hmGSH synthesis from glutathione and L - serine in vitro at acidic pH.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(898)

CPY also catalyzed methylglutathione synthesis from glutathione and L - alanine.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(899)

These findings suggested that a carboxypeptidase - like enzyme may be involved in hmGSH synthesis in vivo.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(900)

Transcriptional elongation by RNA polymerase II and histone methylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	Transcriptional elongation by RNA polymerase II and Proteinhistone Methylationmethylation .

(901)

mRNA synthesis in eukaryotic organisms is a key biological process that is regulated at multiple levels.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(902)

From the covalent modifications of chromatin by a number of chromatin remodeling complexes during the initiation and activation steps of transcription to the processing of mRNA transcripts, a very large consortium of proteins and multiprotein complexes is critical for gene expression by RNA polymerase II.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(903)

The list of proteins essential for the successful synthesis of mRNA continues to grow at a rapid pace.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(904)

Recent advances in this area of research have been focused on transcription through chromatin.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(905)

In this article, we will review the recent literature linking the key biochemical process of transcriptional elongation by RNA polymerase II to histone methylation by COMPASS, Dot1p, and Set2 methyltransferases.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9996	Methylation	In this article, we will review the recent literature linking the key biochemical process of transcriptional elongation by RNA polymerase II to Proteinhistone Methylationmethylation by COMPASS, Dot1p, and Set2 methyltransferases.
0.9995	Methylation	In this article, we will review the recent literature linking the key biochemical process of transcriptional elongation by RNA polymerase II to histone Methylationmethylation by COMPASS, ProteinDot1p , and Set2 methyltransferases.
0.9991	Methylation	In this article, we will review the recent literature linking the key biochemical process of transcriptional elongation by RNA polymerase II to histone Methylationmethylation by COMPASS, Dot1p, and ProteinSet2 methyltransferases.

(906)

Prolyl hydroxylation of collagen type I is required for efficient binding to integrin alpha 1 beta 1 and platelet glycoprotein VI but not to alpha 2 beta 1.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(907)

Collagen is a potent adhesive substrate for cells, an event essentially mediated by the integrins alpha 1 beta 1 and alpha 2 beta 1.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(908)

Collagen fibrils also bind to the integrin alpha 2 beta 1 and the platelet receptor glycoprotein VI to activate and aggregate platelets.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(909)

The distinct triple helical recognition motifs for these receptors, GXOGER and ( GPO ) n, respectively, all contain hydroxyproline.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(910)

Using unhydroxylated collagen I produced in transgenic plants, we investigated the role of hydroxyproline in the receptor - binding properties of collagen.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(911)

We show that alpha 2 beta 1 but not alpha 1 beta 1 mediates cell adhesion to unhydroxylated collagen.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(912)

Soluble recombinant alpha 1 beta 1 binding to unhydroxylated collagen is considerably reduced compared with bovine collagens, but binding can be restored by prolyl hydroxylation of recombinant collagen.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(913)

We also show that platelets use alpha 2 beta 1 to adhere to the unhydroxylated recombinant molecules, but the adhesion is weaker than on fully hydroxylated collagen, and the unhydroxylated collagen fibrils fail to aggregate platelets.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(914)

Prolyl hydroxylation is thus required for binding of collagen to platelet glycoprotein VI and to cells by alpha 1 beta 1.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(915)

These observations give new insights into the molecular basis of collagen - receptor interactions and offer new selective applications for the recombinant unhydroxylated collagen I.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(916)

Mechanism, mutagenesis, and chemical rescue of a beta - mannosidase from cellulomonas fimi.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(917)

The chemical mechanism of a retaining beta - mannosidase from Cellulomonas fimi has been characterized through steady - state kinetic analyses with a range of substrates, coupled with chemical rescue studies on both the wild - type enzyme and mutants in which active site carboxyl groups have been replaced.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(918)

Studies with a series of aryl beta - mannosides of vastly different reactivities ( pK ( a ) ( lg ) = 4 - 10 ) allowed kinetic isolation of the glycosylation and deglycosylation steps.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(919)

Substrate inhibition was observed for all but the least reactive of these substrates.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(920)

Bronsted analysis of k ( cat ) revealed a downward breaking plot ( beta ( lg ) = - 0. 54 + / - 0. 05 ) that is consistent with a change in rate - determining step ( glycosylation to deglycosylation ), and this was confirmed by partitioning studies with ethylene glycol.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(921)

The pH dependence of k ( cat ) / K ( m ) follows an apparent single ionization of a group of pK ( a ) = 7. 65 that must be protonated for catalysis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(922)

The tentative assignment of E429 as the acid - base catalyst of Man2A on the basis of sequence alignments with other family 2 glycosidases was confirmed by the increased turnover rate observed for the mutant E429A in the presence of azide and fluoride, leading to the production of beta - mannosyl azide and beta - mannosyl fluoride, respectively.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(923)

A pH - dependent chemical rescue of E429A activity is also observed with citrate.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(924)

Substantial oxocarbenium ion character at the transition state was demonstrated by the alpha - deuterium kinetic isotope effect for Man2A E429A of alpha - D ( V ) = 1. 12 + / - 0. 01.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(925)

Surprisingly, this isotope effect was substantially greater in the presence of azide ( alpha - D ( V ) = 1. 166 + / - 0. 009 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(926)

Likely involvement of acid / base catalysis was revealed by the pH dependence of k ( cat ) for Man2A E429A, which follows a bell - shaped profile described by pK ( a ) values of 6. 1 and 8. 4, substantially different from that of the wild - type enzyme.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(927)

The glycosidic bond cleaving activity of Man2A E519A and E519S nucleophile mutants is restored with azide and fluoride and appears to correlate with the corresponding " glycosynthase " activities.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(928)

The contribution of the substrate 2 - hydroxyl to stabilization of the Man2A glycosylation transition state ( DeltaDeltaG ( ) = 5. 1 kcal mol ( - 1 ) ) was probed using a 2 - deoxymannose substrate.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(929)

This value, surprisingly, is comparable to that found from equivalent studies with beta - glucosidases despite the geometric differences at C - 2 and the importance of hydrogen bonding at that position.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(930)

Modes of stabilizing the mannosidase transition state are discussed.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(931)

Occurrence of O - linked Xyl - GlcNAc and Xyl - Glc disaccharides in trocarin, a factor Xa homolog from snake venom.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9976	Methylation	Occurrence of GlycosylationO - linked Xyl - GlcNAc and EntityXyl - Glc disaccharides in trocarin, a factor Xa homolog from snake venom.
0.9955	Methylation	Occurrence of GlycosylationO - linked EntityXyl - GlcNAc and Xyl - Glc disaccharides in trocarin, a factor Xa homolog from snake venom.

(932)

Trocarin is a 46515 - Da group D prothrombin - activating glycoprotein from the venom of the Australian elapid, Tropidechis carinatus.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(933)

Amino acid sequencing and functional characterization of trocarin demonstrated that it is a structural and functional homolog of mammalian blood coagulation factor ( F ) Xa.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(934)

In this study we show that, in contrast to mammalian Xa, which is not glycosylated, trocarin contains an O - linked carbohydrate moiety in its light chain and an N - linked carbohydrate oligosaccharide in its heavy chain.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	In this study we show that, in contrast to mammalian ProteinXa , which is not Glycosylationglycosylated , trocarin contains an O - linked carbohydrate moiety in its light chain and an N - linked carbohydrate oligosaccharide in its heavy chain.
0.9902	Methylation	In this study we show that, in contrast to mammalian ProteinXa , which is not glycosylated, trocarin contains an GlycosylationO - linked carbohydrate moiety in its light chain and an N - linked carbohydrate oligosaccharide in its heavy chain.
0.9809	Methylation	In this study we show that, in contrast to mammalian ProteinXa , which is not glycosylated, trocarin contains an O - linked carbohydrate moiety in its light chain and an GlycosylationN - linked carbohydrate oligosaccharide in its heavy chain.

(935)

Mass spectrometry and sugar compositional analysis indicate that the O - linked carbohydrate moiety is a mixture of Xyl - GlcNAc -, GlcNAc -, Xyl - Glc - and Glc - structures linked to Ser 52.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(936)

The N - linked carbohydrate on Asn 45 of the heavy chain is a sialylated, diantennary oligosaccharide that is located at the lip of the active site of the prothrombin activator.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Relation	Text (Head - Tail)
0.6108	Methylation	The GlycosylationN - linked Entitycarbohydrate on Asn 45 of the heavy chain is a sialylated, diantennary oligosaccharide that is located at the lip of the active site of the prothrombin activator.

(937)

Differential glycosylation regulates processing of lipoprotein receptors by gamma - secretase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(938)

The low density lipoprotein ( LDL ) receptor - related protein 1 ( LRP1 ) belongs to a growing number of cell surface proteins that undergo regulated proteolytic processing that culminates in the release of their intracellular domain ( ICD ) by the intramembranous protease gamma - secretase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(939)

Here we show that LRP1 is differentially glycosylated in a tissue - specific manner and that carbohydrate addition reduces proteolytic cleavage of the extracellular domain and, concomitantly, ICD release.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9991	Methylation	Here we show that ProteinLRP1 is differentially Glycosylationglycosylated in a tissue - specific manner and that carbohydrate addition reduces proteolytic cleavage of the extracellular domain and, concomitantly, ICD release.

(940)

The apolipoprotein E ( apoE ) receptor - 2 ( apoER2 ), another member of the LDL receptor family with functions in cellular signal transmission, also undergoes sequential proteolytic processing, resulting in intracellular domain release into the cytoplasm.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(941)

The penultimate processing step also involves cleavage of the apoER2 extracellular domain.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(942)

The rate at which this cleavage step occurs is determined by the glycosylation state of the receptor, which in turn is regulated by the alternative splicing of an exon encoding several O - linked sugar attachment sites.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(943)

These findings suggest a role for differential and tissue - specific glycosylation as a physiological switch that modulates the diverse biological functions of these receptors in a cell - type specific manner.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(944)

Inactivation of p16 gene in leukemia.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(945)

To determine the frequency of p16 gene inactivation in leukemia cells, and to evaluate their value in the prediction of their clinical outcome.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(946)

Bone marrow or peripheral blood samples from 48 patients with leukemia were examined by multiplex polymerase chain reaction ( MPCR ) to detect p16 gene homozygous deletion, and restriction enzyme PCR to detect p16 gene methylation. p16 gene inactivation were detected in 10 of the 48 patients ( 20. 4 % ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9996	Methylation	Bone marrow or peripheral blood samples from 48 patients with leukemia were examined by multiplex polymerase chain reaction ( MPCR ) to detect p16 gene homozygous deletion, and restriction enzyme PCR to detect Proteinp16 gene DNA_methylationmethylation . p16 gene inactivation were detected in 10 of the 48 patients ( 20. 4 % ).

(947)

They were five patients with p16 homozygous deletion, and five patients with p16 methylation, respectively. p16 gene inactivation correlates with adverse prognosis features.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9992	Methylation	They were five patients with p16 homozygous deletion, and five patients with Proteinp16 DNA_methylationmethylation , respectively. p16 gene inactivation correlates with adverse prognosis features.

(948)

The patients with p16 inactivation had poor response to chemotherapy, and had significantly shorter survival times than the patients in whom p16 gene was preserved ( P < 0. 001 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(949)

The inactivation of p16 gene play a key role in the pathogenesis and the progression of some leukemia.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(950)

The detection of p16 gene is reliable prognostic factor that predict shortened survival times.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(951)

A haploid affair : core histone transitions during spermatogenesis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(952)

The process of meiosis reduces a diploid cell to four haploid gametes and is accompanied by extensive recombination.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(953)

Thus, the dynamics of chromatin during meiosis are significantly different than in mitotic cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(954)

As spermatogenesis progresses, there is a widespread reorganization of the haploid genome followed by extensive DNA compaction.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(955)

It has become increasingly clear that the dynamic composition of chromatin plays a critical role in the activities of enzymes and processes that act upon it.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(956)

Therefore, an analysis of the role of histone variants and modifications in these processes may shed light upon the mechanisms involved and the control of chromatin structure in general.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(957)

Histone variants such as histone H3. 3, H2AX, and macroH2A appear to play key roles in the various stages of spermiogenesis, in addition to the specifically modulated acetylation of histone H4 ( acH4 ), ubiquitination of histones H2A and H2B ( uH2A, uH2B ), and phosphorylation of histone H3 ( H3p ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(958)

This review will examine recent discoveries concerning the role of histone modifications and variants during meiosis and spermatogenesis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(959)

HIF prolyl - hydroxylase 2 is the key oxygen sensor setting low steady - state levels of HIF - 1alpha in normoxia.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(960)

Hypoxia - inducible factor ( HIF ), a transcriptional complex conserved from Caenorhabditis elegans to vertebrates, plays a pivotal role in cellular adaptation to low oxygen availability.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(961)

In normoxia, the HIF - alpha subunits are targeted for destruction by prolyl hydroxylation, a specific modification that provides recognition for the E3 ubiquitin ligase complex containing the von Hippel - Lindau tumour suppressor protein ( pVHL ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(962)

Three HIF prolyl - hydroxylases ( PHD1, 2 and 3 ) were identified recently in mammals and shown to hydroxylate HIF - alpha subunits.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(963)

Here we show that specific'silencing'of PHD2 with short interfering RNAs is sufficient to stabilize and activate HIF - 1alpha in normoxia in all the human cells investigated.'Silencing'of PHD1 and PHD3 has no effect on the stability of HIF - 1alpha either in normoxia or upon re - oxygenation of cells briefly exposed to hypoxia.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(964)

We therefore conclude that, in vivo, PHDs have distinct assigned functions, PHD2 being the critical oxygen sensor setting the low steady - state levels of HIF - 1alpha in normoxia.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(965)

Interestingly, PHD2 is upregulated by hypoxia, providing an HIF - 1 - dependent auto - regulatory mechanism driven by the oxygen tension.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(966)

Insulation of the chicken beta - globin chromosomal domain from a chromatin - condensing protein, MENT.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(967)

Active genes are insulated from developmentally regulated chromatin condensation in terminally differentiated cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(968)

We mapped the topography of a terminal stage - specific chromatin - condensing protein, MENT, across the active chicken beta - globin domain.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(969)

We observed two sharp transitions of MENT concentration coinciding with the beta - globin boundary elements.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(970)

The MENT distribution profile was opposite to that of acetylated core histones but correlated with that of histone H3 dimethylated at lysine 9 ( H3me2K9 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	The MENT distribution profile was opposite to that of Acetylationacetylated core Proteinhistones but correlated with that of histone H3 dimethylated at lysine 9 ( H3me2K9 ).
0.9996	Methylation	The MENT distribution profile was opposite to that of acetylated core histones but correlated with that of histone H3 Methylationdimethylated at Entitylysine 9 ( H3me2K9 ).

(971)

Ectopic MENT expression in NIH 3T3 cells caused a large - scale and specific remodeling of chromatin marked by H3me2K9.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(972)

MENT colocalized with H3me2K9 both in chicken erythrocytes and NIH 3T3 cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(973)

Mutational analysis of MENT and experiments with deacetylase inhibitors revealed the essential role of the reaction center loop domain and an inhibitory affect of histone hyperacetylation on the MENT - induced chromatin remodeling in vivo.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9997	Methylation	Mutational analysis of MENT and experiments with deacetylase inhibitors revealed the essential role of the reaction center loop domain and an inhibitory affect of Proteinhistone Acetylationhyperacetylation on the MENT - induced chromatin remodeling in vivo.

(974)

In vitro, the elimination of the histone H3 N - terminal peptide containing lysine 9 by trypsin blocked chromatin self - association by MENT, while reconstitution with dimethylated but not acetylated N - terminal domain of histone H3 specifically restored chromatin self - association by MENT.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Relation	Text (Head - Tail)
0.9995	Methylation	In vitro, the elimination of the histone H3 N - terminal peptide containing lysine 9 by trypsin blocked chromatin self - association by MENT, while reconstitution with dimethylated but not Acetylationacetylated EntityN - terminal domain of histone H3 specifically restored chromatin self - association by MENT.
0.9987	Methylation	In vitro, the elimination of the histone H3 N - terminal peptide containing lysine 9 by trypsin blocked chromatin self - association by MENT, while reconstitution with Methylationdimethylated but not acetylated EntityN - terminal domain of histone H3 specifically restored chromatin self - association by MENT.
0.9994	Methylation	In vitro, the elimination of the histone H3 N - terminal peptide containing lysine 9 by trypsin blocked chromatin self - association by MENT, while reconstitution with dimethylated but not Acetylationacetylated EntityN - terminal domain of histone H3 specifically restored chromatin self - association by MENT.
0.9985	Methylation	In vitro, the elimination of the histone H3 N - terminal peptide containing lysine 9 by trypsin blocked chromatin self - association by MENT, while reconstitution with Methylationdimethylated but not acetylated EntityN - terminal domain of histone H3 specifically restored chromatin self - association by MENT.

(975)

We suggest that histone H3 modification at lysine 9 directly regulates chromatin condensation by recruiting MENT to chromatin in a fashion that is spatially constrained from active genes by gene boundary elements and histone hyperacetylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9991	Methylation	We suggest that histone H3 modification at lysine 9 directly regulates chromatin condensation by recruiting MENT to chromatin in a fashion that is spatially constrained from active genes by gene boundary elements and Proteinhistone Acetylationhyperacetylation .

(976)

Requirement for neo1p in retrograde transport from the Golgi complex to the endoplasmic reticulum.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(977)

Neo1p from Saccharomyces cerevisiae is an essential P - type ATPase and potential aminophospholipid translocase ( flippase ) in the Drs2p family.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(978)

We have previously implicated Drs2p in protein transport steps in the late secretory pathway requiring ADP - ribosylation factor ( ARF ) and clathrin.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(979)

Here, we present evidence that epitope - tagged Neo1p localizes to the endoplasmic reticulum ( ER ) and Golgi complex and is required for a retrograde transport pathway between these organelles.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(980)

Using conditional alleles of NEO1, we find that loss of Neo1p function causes cargo - specific defects in anterograde protein transport early in the secretory pathway and perturbs glycosylation in the Golgi complex.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(981)

Rer1 - GFP, a protein that cycles between the ER and Golgi complex in COPI and COPII vesicles, is mislocalized to the vacuole in neo1 - ts at the nonpermissive temperature.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(982)

These phenotypes suggest that the anterograde protein transport defect is a secondary consequence of a defect in a COPI - dependent retrograde pathway.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(983)

We propose that loss of lipid asymmetry in the cis Golgi perturbs retrograde protein transport to the ER.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(984)

Fabrication of nickel and chromium nanoparticles using the protein cage of apoferritin.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(985)

The iron storage protein, apoferritin, has a cavity in which iron is oxidized and stored as a hydrated oxide core.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(986)

The size of the core is about 7 nm in diameter and is regulated by the cavity size.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(987)

The cavity can be utilized as a nanoreactor to grow inorganic crystals.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(988)

We incubated apoferritin in nickel or chromium salt solutions to fabricate hydroxide nanoparticles in the cavity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(989)

By using a solution containing dissolved carbon dioxide and by precisely controlling the pH, we succeeded in fabricating nickel and chromium cores.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(990)

During the hydroxylation process of nickel ions a large portion of the apoferritin precipitated through bulk precipitation of nickel hydroxide.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(991)

Bulk precipitation was suppressed by adding ammonium ions.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(992)

However, even in the presence of ammonium ions the core did not form using a degassed solution.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(993)

We concluded that carbonate ions were indispensable for core formation and that the ammonium ions prevented precipitation in the bulk solution.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(994)

The optimized condition for nickel core formation was 0. 3 mg / mL horse spleen apoferritin and 5 mM ammonium nickel sulfate in water containing dissolved carbon dioxide.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(995)

The pH was maintained at 8. 65 using two buffer solutions : 150 mM HEPES ( pH 7. 5 ) and 195 mM CAPSO ( pH 9. 5 ) with 20 mM ammonium at 23 degrees C.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(996)

The pH had not changed after 48 h.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(997)

After 24 h of incubation, all apoferritins remained in the supernatant and all of them had cores.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(998)

Recombinant L - ferritin showed less precipitation even above a pH of 8. 65.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(999)

A chromium core was formed under the following conditions : 0. 1 mg / mL apoferritin, 1 mM ammonium chromium sulfate, 100 mM HEPES ( pH 7. 5 ) with a solution containing dissolved carbon dioxide.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1000)

About 80 % of the supernatant apoferritin ( 0. 07 mg / mL ) formed a core.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1001)

In nickel and chromium core formation, carbonate ions would play an important role in accelerating the hydroxylation in the apoferritin cavity compared to the bulk solution outside.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1002)

Histone hyperacetylation in mitosis prevents sister chromatid separation and produces chromosome segregation defects.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	ProteinHistone Acetylationhyperacetylation in mitosis prevents sister chromatid separation and produces chromosome segregation defects.

(1003)

Posttranslational modifications of core histones contribute to driving changes in chromatin conformation and compaction.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1004)

Herein, we investigated the role of histone deacetylation on the mitotic process by inhibiting histone deacetylases shortly before mitosis in human primary fibroblasts.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9997	Methylation	Herein, we investigated the role of Proteinhistone Deacetylationdeacetylation on the mitotic process by inhibiting histone deacetylases shortly before mitosis in human primary fibroblasts.

(1005)

Cells entering mitosis with hyperacetylated histones displayed altered chromatin conformation associated with decreased reactivity to the anti - Ser 10 phospho H3 antibody, increased recruitment of protein phosphatase 1 - delta on mitotic chromosomes, and depletion of heterochromatin protein 1 from the centromeric heterochromatin.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9992	Methylation	Cells entering mitosis with Acetylationhyperacetylated Proteinhistones displayed altered chromatin conformation associated with decreased reactivity to the anti - Ser 10 phospho H3 antibody, increased recruitment of protein phosphatase 1 - delta on mitotic chromosomes, and depletion of heterochromatin protein 1 from the centromeric heterochromatin.

(1006)

Inhibition of histone deacetylation before mitosis produced defective chromosome condensation and impaired mitotic progression in living cells, suggesting that improper chromosome condensation may induce mitotic checkpoint activation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9998	Methylation	Inhibition of Proteinhistone Deacetylationdeacetylation before mitosis produced defective chromosome condensation and impaired mitotic progression in living cells, suggesting that improper chromosome condensation may induce mitotic checkpoint activation.

(1007)

In situ hybridization analysis on anaphase cells demonstrated the presence of chromatin bridges, which were caused by persisting cohesion along sister chromatid arms after centromere separation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1008)

Thus, the presence of hyperacetylated chromatin during mitosis impairs proper chromosome condensation during the pre - anaphase stages, resulting in poor sister chromatid resolution.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1009)

Lagging chromosomes consisting of single or paired sisters were also induced by the presence of hyperacetylated histones, indicating that the less constrained centromeric organization associated with heterochromatin protein 1 depletion may promote the attachment of kinetochores to microtubules coming from both poles.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9989	Methylation	Lagging chromosomes consisting of single or paired sisters were also induced by the presence of Acetylationhyperacetylated Proteinhistones , indicating that the less constrained centromeric organization associated with heterochromatin protein 1 depletion may promote the attachment of kinetochores to microtubules coming from both poles.

(1010)

Hydroxylated kininogens and kinins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9982	Methylation	HydroxylationHydroxylated kininogens and Proteinkinins .
0.9981	Methylation	HydroxylationHydroxylated Proteinkininogens and kinins.

(1011)

Hydroxyprolyl - 3 - bradykinin was identified in the digest of purified human high molecular weight ( H ) kininogen with plasma kallikrein.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1012)

Hydroxyproline was not detected in the heavy and light chains portions of H kininogen, although they include three possible sites for hydroxylation of proline by proline hydroxylase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1013)

The content of hydroxyprolyl - 3 - bradykinin in H kininogen from individual plasmas varied from 14 % to 64 % of total kinin.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1014)

The present results and our previous results indicate that only kinin moity in H kininogen from human and monkey plasmas has been partially hydroxylated post - translationally by proline - 4 - hydroxylase.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Relation	Text (Head - Tail)
0.9989	Methylation	The present results and our previous results indicate that only Proteinkinin moity in H kininogen from human and monkey plasmas has been partially Hydroxylationhydroxylated post - translationally by proline - 4 - hydroxylase.
0.4434	Methylation	The present results and our previous results indicate that only kinin moity in H kininogen from human and monkey plasmas has been partially Hydroxylationhydroxylated post - translationally by Proteinproline - 4 - hydroxylase .

(1015)

Purification and characterization of soluble forms of human and rat stem cell factor recombinantly expressed by Escherichia coli and by Chinese hamster ovary cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1016)

Stem cell factor ( SCF ) is a novel, early - acting hematopoietic factor.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1017)

It was isolated from the medium of a rat cell line in a soluble, processed form ( Zsebo et al., 1990, Cell 63, 195 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1018)

The cloned human and rat genes encode the soluble form plus additional C - terminal amino acids including a hydrophobic transmembrane domain ( Martin et al., 1990, Cell 63, 203 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1019)

We have recombinantly expressed forms of human and rat SCF corresponding to the soluble, processed form in Escherichia coli and in Chinese hamster ovary ( CHO ) cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1020)

After expression in E. coli, folding and oxidation of the SCF polypeptides are required.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1021)

The SCFs expressed in CHO cells are secreted into the medium in active state and, like the natural SCF, are glycosylated.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9995	Methylation	The ProteinSCFs expressed in CHO cells are secreted into the medium in active state and, like the natural SCF, are Glycosylationglycosylated .

(1022)

Purification of the recombinant SCFs is described.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1023)

Biological and biochemical characterization includes activity toward responsive human and mouse cell lines, N - terminal amino acid sequences, disulfide bond linkages, and sites of glycosylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1024)

Inhibition of fusion by neutralizing monoclonal antibodies to the haemagglutinin - neuraminidase glycoprotein of Newcastle disease virus.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1025)

The majority of neutralizing monoclonal antibodies ( MAbs ) to the haemagglutinin - neuraminidase ( HN ) glycoprotein of Newcastle disease virus prevent attachment of the virus to cellular receptors and inhibits virion - induced fusion from without ( FFWO ) and fusion from within ( FFWI ) mediated by the virus glycoprotein - laden infected cell surface.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1026)

For these antibodies, the inhibition of fusion is presumed to be the result of the prevention of HN - mediated bridging of potential fusion partners.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1027)

MAbs against antigenic sites 3 and 4 neutralize virus infectivity, but by a mechanism other than the prevention of attachment, the exact nature of which remains to be established.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1028)

Antibodies to both of these sites effectively inhibit virion - induced FFWO, even when the inducing virus is not infectious.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1029)

This is consistent with the mechanism of neutralization of these MAbs involving the inhibition of an early, post - attachment step in infection.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1030)

MAbs to site 3 also inhibit FFWI, but those to site 4 do not, even when added at high concentrations.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1031)

This suggests that the requirement for HN may be different in the two modes of fusion.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1032)

The epitopes recognized by MAbs to sites 3 and 4 have been delineated by the identification of individual nucleotide substitutions in the HN genes of neutralization escape variants.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1033)

Some of the deduced amino acid substitutions result in additional N - linked glycosylation sites in HN, which are utilized and presumably account for the escape from neutralization.

Precision: 4.76

Recall: 100.00

F1: 9.09

Score	Relation	Text (Head - Tail)
0.9984	Methylation	Some of the deduced amino acid substitutions result in additional EntityN - linked glycosylation sites in HN, which are Glycosylationutilized and presumably account for the escape from neutralization.
0.9991	Methylation	Some of the deduced amino acid substitutions result in additional N - linked Entityglycosylation sites in HN, which are Glycosylationutilized and presumably account for the escape from neutralization.
0.9970	Methylation	Some of the deduced amino acid substitutions result in additional N - linked Entityglycosylation sites in HN, which are Glycosylationutilized and presumably account for the escape from neutralization.
0.9945	Methylation	Some of the deduced amino acid substitutions result in additional EntityN - linked glycosylation sites in HN, which are Glycosylationutilized and presumably account for the escape from neutralization.
0.9625	Methylation	Some of the deduced amino acid substitutions result in additional EntityN - linked glycosylation sites in HN, which are Glycosylationutilized and presumably account for the escape from neutralization.
0.8151	Methylation	Some of the deduced amino acid substitutions result in additional EntityN - linked glycosylation Entityglycosylation sites in HN, which are utilized and presumably account for the escape from neutralization.
0.8138	Methylation	Some of the deduced amino acid substitutions result in additional N - linked Entityglycosylation sites Entityglycosylation sites in HN, which are utilized and presumably account for the escape from neutralization.
0.8119	Methylation	Some of the deduced amino acid substitutions result in additional EntityN - linked glycosylation sites Entityglycosylation sites in HN, which are utilized and presumably account for the escape from neutralization.
0.7828	Methylation	Some of the deduced amino acid substitutions result in additional EntityN - linked Entityglycosylation sites in HN, which are utilized and presumably account for the escape from neutralization.
0.7171	Methylation	Some of the deduced amino acid substitutions result in additional EntityN - linked glycosylation sites EntityN - linked glycosylation sites in HN, which are utilized and presumably account for the escape from neutralization.
0.7153	Methylation	Some of the deduced amino acid substitutions result in additional EntityN - linked glycosylation sites Entityglycosylation sites in HN, which are utilized and presumably account for the escape from neutralization.
0.7102	Methylation	Some of the deduced amino acid substitutions result in additional EntityN - linked glycosylation sites Entityglycosylation sites in HN, which are utilized and presumably account for the escape from neutralization.
0.6894	Methylation	Some of the deduced amino acid substitutions result in additional EntityN - linked glycosylation Entityglycosylation sites in HN, which are utilized and presumably account for the escape from neutralization.
0.6849	Methylation	Some of the deduced amino acid substitutions result in additional EntityN - linked glycosylation sites Entityglycosylation sites in HN, which are utilized and presumably account for the escape from neutralization.
0.6821	Methylation	Some of the deduced amino acid substitutions result in additional N - linked Entityglycosylation Entityglycosylation sites in HN, which are utilized and presumably account for the escape from neutralization.
0.6746	Methylation	Some of the deduced amino acid substitutions result in additional EntityN - linked glycosylation sites EntityN - linked glycosylation sites in HN, which are utilized and presumably account for the escape from neutralization.
0.6448	Methylation	Some of the deduced amino acid substitutions result in additional EntityN - linked Entityglycosylation sites in HN, which are utilized and presumably account for the escape from neutralization.
0.5407	Methylation	Some of the deduced amino acid substitutions result in additional EntityN - linked glycosylation Entityglycosylation sites in HN, which are utilized and presumably account for the escape from neutralization.
0.5377	Methylation	Some of the deduced amino acid substitutions result in additional EntityN - linked glycosylation EntityN - linked glycosylation sites in HN, which are utilized and presumably account for the escape from neutralization.
0.5345	Methylation	Some of the deduced amino acid substitutions result in additional EntityN - linked glycosylation Entityglycosylation sites in HN, which are utilized and presumably account for the escape from neutralization.
0.4927	Methylation	Some of the deduced amino acid substitutions result in additional EntityN - linked glycosylation EntityN - linked glycosylation sites in HN, which are utilized and presumably account for the escape from neutralization.

(1034)

The influence of N - linked glycosylation on the antigenicity and immunogenicity of rubella virus E1 glycoprotein.

Precision: 25.00

Recall: 100.00

F1: 40.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	The influence of GlycosylationN - linked glycosylation on the antigenicity and immunogenicity of rubella virus ProteinE1 glycoprotein.
0.9999	Methylation	The influence of N - linked Glycosylationglycosylation on the antigenicity and immunogenicity of rubella virus ProteinE1 glycoprotein.
0.8685	Methylation	The influence of GlycosylationN - linked glycosylation Glycosylationglycosylation on the antigenicity and immunogenicity of rubella virus E1 glycoprotein.
0.8347	Methylation	The influence of GlycosylationN - linked glycosylation Glycosylationglycosylation on the antigenicity and immunogenicity of rubella virus E1 glycoprotein.

(1035)

Rubella virus E1 glycoprotein contains three functional N - linked glycosylation sites.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1036)

The role of N - linked glycosylation on the antigenicity and immunogenicity of E1 glycoprotein was studied using vaccinia recombinants expressing E1 glycosylation mutants.

Precision: 11.11

Recall: 100.00

F1: 20.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	The role of GlycosylationN - linked glycosylation on the antigenicity and immunogenicity of ProteinE1 glycoprotein was studied using vaccinia recombinants expressing E1 glycosylation mutants.
0.9999	Methylation	The role of N - linked Glycosylationglycosylation on the antigenicity and immunogenicity of ProteinE1 glycoprotein was studied using vaccinia recombinants expressing E1 glycosylation mutants.
0.9783	Methylation	The role of GlycosylationN - linked glycosylation on the antigenicity and immunogenicity of ProteinE1 glycoprotein was studied using vaccinia recombinants expressing E1 glycosylation mutants.
0.8596	Methylation	The role of GlycosylationN - linked glycosylation Glycosylationglycosylation on the antigenicity and immunogenicity of E1 glycoprotein was studied using vaccinia recombinants expressing E1 glycosylation mutants.
0.7845	Methylation	The role of GlycosylationN - linked glycosylation Glycosylationglycosylation on the antigenicity and immunogenicity of E1 glycoprotein was studied using vaccinia recombinants expressing E1 glycosylation mutants.
0.7073	Methylation	The role of GlycosylationN - linked Glycosylationglycosylation on the antigenicity and immunogenicity of E1 glycoprotein was studied using vaccinia recombinants expressing E1 glycosylation mutants.
0.6493	Methylation	The role of GlycosylationN - linked GlycosylationN - linked glycosylation on the antigenicity and immunogenicity of E1 glycoprotein was studied using vaccinia recombinants expressing E1 glycosylation mutants.
0.6315	Methylation	The role of GlycosylationN - linked Glycosylationglycosylation on the antigenicity and immunogenicity of E1 glycoprotein was studied using vaccinia recombinants expressing E1 glycosylation mutants.
0.6080	Methylation	The role of GlycosylationN - linked glycosylation GlycosylationN - linked glycosylation on the antigenicity and immunogenicity of E1 glycoprotein was studied using vaccinia recombinants expressing E1 glycosylation mutants.

(1037)

Expressed E1 glycosylation mutant proteins were recognized by a panel of E1 - specific monoclonal antibodies in radioimmunoprecipitation, immunofluorescence, and immunoblotting, indicating that carbohydrate side chains on E1 are not involved in the constitution of epitopes recognized by these monoclonal antibodies.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1038)

This observation was further supported by the fact that removal of oligosaccharides on E1 by glycosidase digestion did not significantly change the antigenicity of E1.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1039)

All the glycosylation mutants were capable of eliciting anti - RV E1 antibodies.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1040)

The single glycosylation mutants ( G1, G2, and G3 ), but not the double mutant ( G23 ) or the triple mutant ( G123 ), were found to be capable of inducing virus neutralizing antibodies.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1041)

Among the single glycosylation mutants, only G2 and G3 were active in producing hemagglutination inhibition antibodies in mice.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1042)

Our findings suggest that although carbohydrate on E1 is not directly involved in the antigenic structures of E1, it is important in maintaining proper protein folding and stable conformation for expression of immunological epitopes on E1.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1043)

Glycosylation of the interleukin - 1 receptor type I is required for optimal binding of interleukin - 1.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9996	Methylation	GlycosylationGlycosylation of the Proteininterleukin - 1 receptor type I is required for optimal binding of interleukin - 1.

(1044)

The two types of cell surface receptors for interleukin - 1 ( IL - 1 ) are glycoproteins that contain N - glycosidic chains on their extracellular portions.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1045)

To determine the role of glycosylation of the IL - 1 receptor type I ( IL - 1RtI ) in the binding and function of IL - 1, we used four plant lectins and glycosidase treatment on two different T - cell lines ( EL4 - 6. 1 and D10S ) and expressing high number of binding sites for IL - 1.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9996	Methylation	To determine the role of Glycosylationglycosylation of the ProteinIL - 1 receptor type I ( IL - 1RtI ) in the binding and function of IL - 1, we used four plant lectins and glycosidase treatment on two different T - cell lines ( EL4 - 6. 1 and D10S ) and expressing high number of binding sites for IL - 1.

(1046)

The lectins wheat germ agglutinin, phytohemagglutinin, and concanavalin A inhibited in a dose - response manner the IL - 1 - induced proliferation of D10S cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1047)

Binding of IL - 1 was blocked and radioactive IL - 1 was displaced from these cells by these lectins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1048)

Specific sugars ( GlcNAc, NeuAc, Gal - GlcNAc - Man, Man, or alpha - MeMan ) did not themselves affect IL - 1 binding but reversed the blocking effects of the lectins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1049)

The two cell lines differed in their responses to the lectin - mediated inhibition of IL - 1 binding.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1050)

Digestion by N - glycosidase significantly decreased the capacity of cells to bind IL - 1, and reduced by approximately 20, 000 D the M ( r ) of the IL - 1RtI.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1051)

Neuraminidase and O - glycanase treatment did not alter the binding of IL - 1 to D10S or EL4 - 6. 1 cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1052)

This study demonstrates that glycosylation of the extracellular domain of the IL - 1RtI is due to N - linked carbohydrates, that the degree of glycosylation can vary in cells of different lineage, and that this N - linked glycosylation appears to be essential for optimal binding and activity of IL - 1 to its type I receptor.

Precision: 25.00

Recall: 100.00

F1: 40.00

Score	Relation	Text (Head - Tail)
0.9923	Methylation	This study demonstrates that glycosylation of the extracellular domain of the ProteinIL - 1RtI is due to N - linked carbohydrates, that the degree of Glycosylationglycosylation can vary in cells of different lineage, and that this N - linked glycosylation appears to be essential for optimal binding and activity of IL - 1 to its type I receptor.
0.9541	Methylation	This study demonstrates that glycosylation of the extracellular domain of the ProteinIL - 1RtI is due to N - linked carbohydrates, that the degree of glycosylation can vary in cells of different lineage, and that this GlycosylationN - linked glycosylation appears to be essential for optimal binding and activity of IL - 1 to its type I receptor.
0.8956	Methylation	This study demonstrates that Glycosylationglycosylation of the extracellular domain of the IL - 1RtI is due to N - linked Entitycarbohydrates , that the degree of glycosylation can vary in cells of different lineage, and that this N - linked glycosylation appears to be essential for optimal binding and activity of IL - 1 to its type I receptor.
0.9473	Methylation	This study demonstrates that glycosylation of the extracellular domain of the ProteinIL - 1RtI is due to N - linked carbohydrates, that the degree of glycosylation can vary in cells of different lineage, and that this N - linked Glycosylationglycosylation appears to be essential for optimal binding and activity of IL - 1 to its type I receptor.
0.9015	Methylation	This study demonstrates that glycosylation of the extracellular domain of the ProteinIL - 1RtI is due to GlycosylationN - linked carbohydrates, that the degree of glycosylation can vary in cells of different lineage, and that this N - linked glycosylation appears to be essential for optimal binding and activity of IL - 1 to its type I receptor.
0.8832	Methylation	This study demonstrates that glycosylation of the extracellular domain of the ProteinIL - 1RtI is due to N - linked carbohydrates, that the degree of glycosylation can vary in cells of different lineage, and that this GlycosylationN - linked glycosylation appears to be essential for optimal binding and activity of IL - 1 to its type I receptor.
0.8784	Methylation	This study demonstrates that glycosylation of the extracellular domain of the IL - 1RtI is due to N - linked carbohydrates, that the degree of glycosylation can vary in cells of different lineage, and that this GlycosylationN - linked glycosylation Glycosylationglycosylation appears to be essential for optimal binding and activity of IL - 1 to its type I receptor.
0.8477	Methylation	This study demonstrates that glycosylation of the extracellular domain of the IL - 1RtI is due to N - linked carbohydrates, that the degree of glycosylation can vary in cells of different lineage, and that this GlycosylationN - linked glycosylation Glycosylationglycosylation appears to be essential for optimal binding and activity of IL - 1 to its type I receptor.
0.7924	Methylation	This study demonstrates that glycosylation of the extracellular domain of the IL - 1RtI is due to N - linked carbohydrates, that the degree of glycosylation can vary in cells of different lineage, and that this GlycosylationN - linked Glycosylationglycosylation appears to be essential for optimal binding and activity of IL - 1 to its type I receptor.
0.7901	Methylation	This study demonstrates that glycosylation of the extracellular domain of the IL - 1RtI is due to N - linked carbohydrates, that the degree of glycosylation can vary in cells of different lineage, and that this GlycosylationN - linked GlycosylationN - linked glycosylation appears to be essential for optimal binding and activity of IL - 1 to its type I receptor.
0.7245	Methylation	This study demonstrates that glycosylation of the extracellular domain of the IL - 1RtI is due to N - linked carbohydrates, that the degree of glycosylation can vary in cells of different lineage, and that this GlycosylationN - linked Glycosylationglycosylation appears to be essential for optimal binding and activity of IL - 1 to its type I receptor.
0.6665	Methylation	This study demonstrates that glycosylation of the extracellular domain of the IL - 1RtI is due to N - linked carbohydrates, that the degree of glycosylation can vary in cells of different lineage, and that this GlycosylationN - linked glycosylation GlycosylationN - linked glycosylation appears to be essential for optimal binding and activity of IL - 1 to its type I receptor.

(1053)

Expression of Cpgp40 / 15 in Toxoplasma gondii : a surrogate system for the study of Cryptosporidium glycoprotein antigens.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1054)

Cryptosporidium parvum is a waterborne enteric coccidian that causes diarrheal disease in a wide range of hosts.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1055)

Development of successful therapies is hampered by the inability to culture the parasite and the lack of a transfection system for genetic manipulation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1056)

The glycoprotein products of the Cpgp40 / 15 gene, gp40 and gp15, are involved in C. parvum sporozoite attachment to and invasion of host cells and, as such, may be good targets for anticryptosporidial therapies.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1057)

However, the function of these antigens appears to be dependent on the presence of multiple O - linked alpha - N - acetylgalactosamine ( alpha - GalNAc ) determinants.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Relation	Text (Head - Tail)
0.9986	Methylation	However, the function of these antigens appears to be dependent on the presence of multiple GlycosylationO - linked Entityalpha - N - acetylgalactosamine ( alpha - GalNAc ) determinants.
0.8171	Methylation	However, the function of these antigens appears to be dependent on the presence of multiple GlycosylationO - linked Entityalpha - N - acetylgalactosamine ( alpha - GalNAc ) determinants.

(1058)

A eukaryotic expression system that would produce proteins bearing glycosylation patterns similar to those found on the native C. parvum glycoproteins would greatly facilitate the molecular and functional characterization of these antigens.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1059)

As a unique approach to this problem, the Cpgp40 / 15 gene was transiently expressed in Toxoplasma gondii, and the expressed recombinant glycoproteins were characterized.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1060)

Antisera to gp40 and gp15 reacted with the surface membranes of tachyzoites expressing the Cpgp40 / 15 construct, and this reactivity colocalized with that of antiserum to the T. gondii surface protein SAG1.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1061)

Surface membrane localization was dependent on the presence of the glycophosphatidylinositol anchor attachment site present in the gp15 coding sequence.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1062)

The presence of terminal O - linked alpha - GalNAc determinants on the T. gondii recombinant gp40 was confirmed by reactivity with Helix pomatia lectin and the monoclonal antibody 4E9, which recognizes alpha - GalNAc residues, and digestion with alpha - N - acetylgalactosaminidase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9982	Methylation	The presence of terminal GlycosylationO - linked Entityalpha - GalNAc determinants on the T. gondii recombinant gp40 was confirmed by reactivity with Helix pomatia lectin and the monoclonal antibody 4E9, which recognizes alpha - GalNAc residues, and digestion with alpha - N - acetylgalactosaminidase.

(1063)

In addition to appropriate localization and glycosylation, T. gondii apparently processes the gp40 / 15 precursor into the gp40 and gp15 component glycopolypeptides, albeit inefficiently.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1064)

These results suggest that a surrogate system using T. gondii for the study of Cryptosporidium biology may be useful.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1065)

Aberrant methylation of DAP - kinase in therapy - related acute myeloid leukemia and myelodysplastic syndromes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	Aberrant DNA_methylationmethylation of ProteinDAP - kinase in therapy - related acute myeloid leukemia and myelodysplastic syndromes.

(1066)

Death - associated protein kinase ( DAP - kinase ), a proapoptotic serine / threonine kinase, is a candidate tumor suppressor gene.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1067)

We studied the methylation status of DAP - kinase of 194 bone marrow samples from 160 patients with acute myeloid leukemia ( AML ) and 34 with a myelodysplastic syndrome ( MDS ) at the time of initial diagnosis by polymerase chain reaction ( PCR ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	We studied the DNA_methylationmethylation status of ProteinDAP - kinase of 194 bone marrow samples from 160 patients with acute myeloid leukemia ( AML ) and 34 with a myelodysplastic syndrome ( MDS ) at the time of initial diagnosis by polymerase chain reaction ( PCR ).

(1068)

Hypermethylation of DAP - kinase was present in 27. 5 % ( 44 of 160 ) of AML and in 47 % ( 16 of 34 ) of MDS specimens and significantly correlated to loss of DAP - kinase expression ( P =. 008 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	DNA_methylationHypermethylation of ProteinDAP - kinase was present in 27. 5 % ( 44 of 160 ) of AML and in 47 % ( 16 of 34 ) of MDS specimens and significantly correlated to loss of DAP - kinase expression ( P =. 008 ).

(1069)

It was significantly more frequent in AML secondary to therapy for other malignancies ( s - AML ; 14 of 29, 48. 3 % ), as compared to de novo AML ( 30 of 131, 22. 9 %, P =. 01 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1070)

DAP - kinase hypermethylation in AML was associated with myelodysplastic changes in the bone marrow at the time of the initial diagnosis ( P =. 002 ) and with the presence of cytogenetic abnormalities ( P =. 02 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9998	Methylation	ProteinDAP - kinase DNA_methylationhypermethylation in AML was associated with myelodysplastic changes in the bone marrow at the time of the initial diagnosis ( P =. 002 ) and with the presence of cytogenetic abnormalities ( P =. 02 ).

(1071)

Alteration in the apoptotic response due to the loss of DAP - kinase function may be an early event in the transformation pathway to secondary leukemia via myelodysplasia.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1072)

Molecular pathology of atypical polypoid adenomyoma of the uterus.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1073)

Atypical polypoid adenomyoma ( APA ) is an uncommon and benign tumor of the uterus.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1074)

In some patients, however, APA has been found to coexist with or to precede the development of an endometrioid adenocarcinoma similarly to complex endometrial hyperplasia.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1075)

The molecular changes underlying the progression from APA to adenocarcinoma are unknown.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1076)

DNA from paraffin - embedded tissue of 6 APAs was evaluated for microsatellite instability ( MI ), MLH - 1 promoter hypermethylation, and CTNNB - 1 mutations.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9941	Methylation	DNA from paraffin - embedded tissue of 6 APAs was evaluated for microsatellite instability ( MI ), MLH - 1 Entitypromoter DNA_methylationhypermethylation , and CTNNB - 1 mutations.

(1077)

Tissue sections were also subjected to MLH - 1, MSH - 2, and beta - catenin immunostaining.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1078)

MI was not detected in any case.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1079)

Two tumors exhibited MLH - 1 promoter hypermethylation and showed focal negative MHL - 1 immunostaining ; 1 of these showed marked architectural complexity and cellular pleomorphism.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9944	Methylation	Two tumors exhibited MLH - 1 Entitypromoter DNA_methylationhypermethylation and showed focal negative MHL - 1 immunostaining ; 1 of these showed marked architectural complexity and cellular pleomorphism.

(1080)

Five cases presented beta - catenin nuclear immunoreactivity, but none of them had CTNNB - 1 mutations.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1081)

The results of this study suggest that APA and complex endometrial hyperplasia may share some molecular alterations.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1082)

Some APAs exhibit MLH - 1 promoter hypermethylation with focal lack of MLH - 1 immunostaining, a molecular abnormality involved in the transition from complex atypical hyperplasia to endometrioid adenocarcinoma.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9973	Methylation	Some APAs exhibit MLH - 1 Entitypromoter DNA_methylationhypermethylation with focal lack of MLH - 1 immunostaining, a molecular abnormality involved in the transition from complex atypical hyperplasia to endometrioid adenocarcinoma.

(1083)

A common polymorphism in the oxygen - dependent degradation ( ODD ) domain of hypoxia inducible factor - 1alpha ( HIF - 1alpha ) does not impair Pro - 564 hydroxylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9986	Methylation	A common polymorphism in the oxygen - dependent degradation ( ODD ) domain of hypoxia inducible factor - 1alpha ( HIF - 1alpha ) does not impair EntityPro - 564 Hydroxylationhydroxylation .

(1084)

BACKGROUND : The hypoxia - inducible factor ( HIF ) transcription complex, which is activated by low oxygen tension, controls a diverse range of cellular processes including angiogenesis and erythropoiesis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1085)

Under normoxic conditions, the alpha subunit of HIF is rapidly degraded in a manner dependent on hydroxylation of two conserved proline residues at positions 402 and 564 in HIF - 1alpha in the oxygen - dependent degradation ( ODD ) domain.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Relation	Text (Head - Tail)
1.0000	Methylation	Under normoxic conditions, the alpha subunit of HIF is rapidly degraded in a manner dependent on Hydroxylationhydroxylation of two conserved Entityproline residues at positions 402 and 564 in HIF - 1alpha in the oxygen - dependent degradation ( ODD ) domain.
0.9999	Methylation	Under normoxic conditions, the alpha subunit of HIF is rapidly degraded in a manner dependent on Hydroxylationhydroxylation of two conserved Entityproline residues at positions 402 and 564 in HIF - 1alpha in the oxygen - dependent degradation ( ODD ) domain.

(1086)

This allows subsequent recognition by the von Hippel - Lindau ( VHL ) tumor suppressor protein, which targets HIF for degradation by the ubiquitin - proteasome pathway.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1087)

Under hypoxic conditions, prolyl hydroxylation of HIF is inhibited, allowing it to escape VHL - mediated degradation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1088)

The transcriptional regulation of the erythropoietin gene by HIF raises the possibility that HIF may play a role in disorders of erythropoiesis, such as idiopathic erythrocytosis ( IE ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1089)

RESULTS : Patients with IE were screened for changes in the HIF - 1alpha coding sequence, and a change in the ODD domain that converts Pro - 582 to Ser was identified in several patients.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1090)

This same change, however, was also detected at a significant frequency, 0. 073, in unaffected controls compared to 0. 109 in the IE patient group.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1091)

In vitro hydroxylation assays examining this amino acid change failed to reveal a discernible effect on HIF hydroxylation at Pro - 564.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1092)

CONCLUSION : The Pro582Ser change represents a common polymorphism of HIF - 1alpha that does not impair HIF - 1alpha prolyl hydroxylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9975	Methylation	CONCLUSION : The Pro582Ser change represents a common polymorphism of HIF - 1alpha that does not impair HIF - 1alpha Entityprolyl Hydroxylationhydroxylation .

(1093)

Although the Pro582Ser polymorphism is located in the ODD domain of HIF - 1alpha it does not diminish the association of HIF - 1alpha with VHL.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1094)

Thus, it is unlikely that this polymorphism accounts for the erythrocytosis in the group of IE patients studied.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1095)

Expression of histone acetyltransferases was down - regulated in poly ( ADP - ribose ) polymerase - 1 - deficient murine cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1096)

NF - kappaB - dependent, as well as human immunodeficiency virus type - 1 ( HIV - 1 ) long terminal repeat ( LTR ) - dependent, reporter gene expression was significantly impaired in cells derived from poly ( ADP - ribose ) polymerase - 1 ( PARP - 1 ) - knockout ( PARP - 1 - / - ) mice.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1097)

In addition, the level of protein acetylation was markedly lower in PARP - 1 - / - cells than control ( PARP - 1 + / + ) cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1098)

Surprisingly, the expression levels of histone acetyltransferases ( HATs ), p300, cAMP response element - binding protein - binding protein ( CBP ), and p300 / CBP - associated factor ( PCAF ), were significantly reduced in PARP - 1 - / - cells, as compared with PARP - 1 + / + cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1099)

These results suggest that PARP - 1 is required for the proper expression of particular HATs.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1100)

Since p300 and CBP are coactivators of NF - kappaB, we propose here that PARP - 1 participates in NF - kappaB - dependent transcription by means of maintaining the expression of HATs.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1101)

The ITIM - bearing CLECSF6 ( DCIR ) is down - modulated in neutrophils by neutrophil activating agents.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1102)

The recently discovered CLECSF6 protein displays the features of a receptor involved in the down - modulation of leukocyte activation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1103)

Although CLECSF6 has been the focus of the interest of many researchers lately, a Western blot characterization of the protein is still lacking.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1104)

This highly reduces our ability to gain full knowledge of the biological relevance of this protein in cell responses.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1105)

We produced two rabbit polyclonal antisera that detected a glycosylated protein at approximately 35kDa in neutrophils.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1106)

Four different CLECSF6 mRNA species have been discovered to date.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1107)

When deglycosylated, the protein displayed the molecular weight expected for the longest CLECSF6 form.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	When Deglycosylationdeglycosylated , the protein displayed the molecular weight expected for the longest ProteinCLECSF6 form.

(1108)

Neutrophil membrane fractions were strongly enriched in the protein.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1109)

We showed a down - modulation of the expression of this protein in neutrophils treated with granulocyte - macrophage - colony stimulating factor ( GM - CSF ), tumor necrosis factor ( TNF - alpha ), lipopolysaccharide ( LPS ), and interleukin ( IL ) - 4.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1110)

This work supports the hypothesis that CLECSF6 is involved in the control of inflammation in neutrophils.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1111)

Epigenetic switch from posttranscriptional to transcriptional silencing is correlated with promoter hypermethylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1112)

Changes in the distribution of methylcytosine residues along a transgene locus of tobacco ( Nicotiana tabacum ) in relation to the type of gene silencing were studied in parental plant leaves, calli, and regenerated plants derived thereof.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1113)

Parental - silenced HeLo1 ( hemizygous for locus 1 ) plants show posttranscriptional silencing of the residing nptII ( neomycin phosphotransferase II ) transgene and cytosine methylation restricted to the 3'end and center part of the transcribed region.

Precision: 33.33

Recall: 100.00

F1: 50.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	Parental - silenced HeLo1 ( hemizygous for locus 1 ) plants show posttranscriptional silencing of the residing nptII ( neomycin phosphotransferase II ) transgene and cytosine DNA_methylationmethylation restricted to the Entity3'end and center part of the transcribed region.
0.9997	Methylation	Parental - silenced HeLo1 ( hemizygous for locus 1 ) plants show posttranscriptional silencing of the residing nptII ( neomycin phosphotransferase II ) transgene and cytosine DNA_methylationmethylation restricted to the 3'end and Entitycenter part of the transcribed region.
0.9999	Methylation	Parental - silenced HeLo1 ( hemizygous for locus 1 ) plants show posttranscriptional silencing of the residing nptII ( neomycin phosphotransferase II ) transgene and cytosine DNA_methylationmethylation restricted to the 3 ' Entityend and center part of the transcribed region.
0.9999	Methylation	Parental - silenced HeLo1 ( hemizygous for locus 1 ) plants show posttranscriptional silencing of the residing nptII ( neomycin phosphotransferase II ) transgene and cytosine DNA_methylationmethylation restricted to the Entity3 ' end and center part of the transcribed region.
0.9997	Methylation	Parental - silenced HeLo1 ( hemizygous for locus 1 ) plants show posttranscriptional silencing of the residing nptII ( neomycin phosphotransferase II ) transgene and cytosine DNA_methylationmethylation restricted to the 3'end and center part of the Entitytranscribed region .
0.9994	Methylation	Parental - silenced HeLo1 ( hemizygous for locus 1 ) plants show posttranscriptional silencing of the residing nptII ( neomycin phosphotransferase II ) transgene and cytosine DNA_methylationmethylation restricted to the 3'end and Entitycenter part of the transcribed region.

(1114)

Here, we report that with an increasing number of cell cycles, DNA methylation changes gradually, and methylation is introduced into the promoter during cell culture and more slowly in vegetatively propagated plants.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1115)

After 24 months of callus in vitro cultivation, an epigenetic variant, designated locus 1E, was obtained in which cytosine methylation of symmetrical ( CG and CNG ) sites was almost complete within the 5'end of the nptII - transcribed region and the 35S promoter.

Precision: 28.57

Recall: 100.00

F1: 44.44

Score	Relation	Text (Head - Tail)
0.9998	Methylation	After 24 months of callus in vitro cultivation, an epigenetic variant, designated locus 1E, was obtained in which cytosine DNA_methylationmethylation of symmetrical ( CG and CNG ) sites was almost complete within the Entity5'end of the nptII - transcribed region and the 35S promoter.
0.9986	Methylation	After 24 months of callus in vitro cultivation, an epigenetic variant, designated locus 1E, was obtained in which cytosine DNA_methylationmethylation of symmetrical ( CG and CNG ) sites was almost complete within the 5'end of the nptII - transcribed region and the Entity35S promoter .
0.9997	Methylation	After 24 months of callus in vitro cultivation, an epigenetic variant, designated locus 1E, was obtained in which cytosine DNA_methylationmethylation of symmetrical ( CG and CNG ) sites was almost complete within the Entity5 ' end of the nptII - transcribed region and the 35S promoter.
0.9997	Methylation	After 24 months of callus in vitro cultivation, an epigenetic variant, designated locus 1E, was obtained in which cytosine DNA_methylationmethylation of symmetrical ( CG and CNG ) sites was almost complete within the 5 ' Entityend of the nptII - transcribed region and the 35S promoter.
0.9994	Methylation	After 24 months of callus in vitro cultivation, an epigenetic variant, designated locus 1E, was obtained in which cytosine DNA_methylationmethylation of symmetrical ( CG and CNG ) sites was almost complete within the 5'end of the nptII - transcribed Entityregion and the 35S promoter.
0.9990	Methylation	After 24 months of callus in vitro cultivation, an epigenetic variant, designated locus 1E, was obtained in which cytosine DNA_methylationmethylation of symmetrical ( CG and CNG ) sites was almost complete within the 5'end of the nptII - transcribed region and the 35S Entitypromoter .
0.9980	Methylation	After 24 months of callus in vitro cultivation, an epigenetic variant, designated locus 1E, was obtained in which cytosine DNA_methylationmethylation of symmetrical ( CG and CNG ) sites was almost complete within the 5'end of the nptII - transcribed region and the Entity35S promoter.

(1116)

Further, methylation of nonsymmetrical sites appeared de novo in the promoter, whereas this type of methylation was significantly reduced in the 3'end of the transcribed region when compared with locus 1.

Precision: 25.00

Recall: 100.00

F1: 40.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	Further, DNA_methylationmethylation of nonsymmetrical sites appeared de novo in the Entitypromoter , whereas this type of methylation was significantly reduced in the 3'end of the transcribed region when compared with locus 1.
0.9981	Methylation	Further, DNA_methylationmethylation of nonsymmetrical sites appeared de novo in the promoter, whereas this type of methylation was significantly reduced in the 3'end of the Entitytranscribed region when compared with locus 1.
0.9993	Methylation	Further, methylation of nonsymmetrical sites appeared de novo in the promoter, whereas this type of DNA_methylationmethylation was significantly reduced in the Entity3 ' end of the transcribed region when compared with locus 1.
0.9987	Methylation	Further, methylation of nonsymmetrical sites appeared de novo in the promoter, whereas this type of DNA_methylationmethylation was significantly reduced in the 3'end of the Entitytranscribed region when compared with locus 1.
0.9978	Methylation	Further, methylation of nonsymmetrical sites appeared de novo in the promoter, whereas this type of DNA_methylationmethylation was significantly reduced in the 3'end of the Entitytranscribed region when compared with locus 1.
0.9967	Methylation	Further, DNA_methylationmethylation of nonsymmetrical sites appeared de novo in the promoter, whereas this type of methylation was significantly reduced in the 3'end of the Entitytranscribed region when compared with locus 1.
0.9966	Methylation	Further, DNA_methylationmethylation of nonsymmetrical sites appeared de novo in the promoter, whereas this type of methylation was significantly reduced in the Entity3 ' end of the transcribed region when compared with locus 1.
0.9929	Methylation	Further, methylation of nonsymmetrical sites appeared de novo in the Entitypromoter , whereas this type of DNA_methylationmethylation was significantly reduced in the 3'end of the transcribed region when compared with locus 1.

(1117)

The newly established epigenetic patterns were stably transmitted from calli into regenerated plants and their progeny.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1118)

The protein and steady - state RNA levels remained low in locus 1E, whereas with nuclear run - on assays, no detectable amounts of primary transcripts were found along the nptII gene, indicating that the methylated promoter became inactivated.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9972	Methylation	The protein and steady - state RNA levels remained low in locus 1E, whereas with nuclear run - on assays, no detectable amounts of primary transcripts were found along the nptII gene, indicating that the DNA_methylationmethylated Entitypromoter became inactivated.

(1119)

The results suggest that a switch between posttranscriptional and transcriptional gene silencing could be a mechanism leading to irrevocable shut down of gene expression within a finite number of generations.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1120)

Frequent methylation of p16INK4A and p14ARF genes implicated in the evolution of chronic myeloid leukaemia from its chronic to accelerated phase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	Frequent DNA_methylationmethylation of Proteinp16INK4A and p14ARF genes implicated in the evolution of chronic myeloid leukaemia from its chronic to accelerated phase.
0.9999	Methylation	Frequent DNA_methylationmethylation of p16INK4A and Proteinp14ARF genes implicated in the evolution of chronic myeloid leukaemia from its chronic to accelerated phase.

(1121)

The frequency and mechanism of p16 ( INK4A ) and p14 ( ARF ) gene alterations were studied in cell samples from 30 patients with Philadelphia ( Ph ) chromosome - positive chronic myeloid leukaemia ( CML ), both at diagnosis and at the onset of the accelerated phase ( AP ) of the disease.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1122)

No alterations in the p16 ( INK4A ) or p14 ( ARF ) genes were found in any of the chronic phase ( CP ) samples.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1123)

DNA sequencing analyses detected p16 ( INK4A ) or p14 ( ARF ) mutations in 17 AP samples.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1124)

All mutations were heterozygous without loss of the other allele.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1125)

Aberrant methylation of the p16 ( INK4A ) or p14 ( ARF ) promoters was found in 14 of 30 AP samples.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9958	Methylation	Aberrant DNA_methylationmethylation of the p16 ( INK4A ) or p14 ( ARF ) Entitypromoters was found in 14 of 30 AP samples.

(1126)

The most common situation was the simultaneous methylation of both promoters.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9996	Methylation	The most common situation was the simultaneous DNA_methylationmethylation of both Entitypromoters .

(1127)

Our data indicate that p16 ( INK4A ) and p14 ( ARF ) are primary targets for inactivation by promoter methylation in the acceleration of CML.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9984	Methylation	Our data indicate that p16 ( INK4A ) and p14 ( ARF ) are primary targets for inactivation by Entitypromoter DNA_methylationmethylation in the acceleration of CML.

(1128)

Transcriptional silencing of the p16 ( INK4A ) and p14 ( ARF ) genes may be important in the conversion of CML from the CP to the AP.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1129)

mSin3A / histone deacetylase 2 - and PRMT5 - containing Brg1 complex is involved in transcriptional repression of the Myc target gene cad.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1130)

The role of hSWI / SNF complexes in transcriptional activation is well characterized ; however, little is known about their function in transcriptional repression.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1131)

We have previously shown that subunits of the mSin3A / histone deacetylase 2 ( HDAC2 ) corepressor complex copurify with hSWI / SNF complexes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1132)

Here we show that the type II arginine - specific methyltransferase PRMT5, which is involved in cyclin E repression, can be found in association with Brg1 and hBrm - based hSWI / SNF complexes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1133)

We also show that hSWI / SNF - associated PRMT5 can methylate hypoacetylated histones H3 and H4 more efficiently than hyperacetylated histones H3 and H4.

Precision: 50.00

Recall: 77.78

F1: 60.87

Score	Relation	Text (Head - Tail)
1.0000	Methylation	We also show that hSWI / SNF - associated PRMT5 can Methylationmethylate hypoacetylated Proteinhistones H3 and H4 more efficiently than hyperacetylated histones H3 and H4.
0.9999	Methylation	We also show that hSWI / SNF - associated PRMT5 can Methylationmethylate hypoacetylated histones H3 and ProteinH4 more efficiently than hyperacetylated histones H3 and H4.
0.9989	Methylation	We also show that hSWI / SNF - associated ProteinPRMT5 can Methylationmethylate hypoacetylated histones H3 and H4 more efficiently than hyperacetylated histones H3 and H4.
0.9976	Methylation	We also show that hSWI / SNF - associated PRMT5 can methylate hypoacetylated histones H3 and H4 more efficiently than Acetylationhyperacetylated histones H3 and ProteinH4 .
0.9921	Methylation	We also show that hSWI / SNF - associated PRMT5 can methylate hypoacetylated histones H3 and H4 more efficiently than Acetylationhyperacetylated Proteinhistones H3 and H4.
0.9885	Methylation	We also show that hSWI / SNF - associated PRMT5 can Methylationmethylate hypoacetylated histones H3 and H4 more efficiently than hyperacetylated histones H3 and ProteinH4 .
0.9769	Methylation	We also show that hSWI / SNF - associated PRMT5 can Methylationmethylate hypoacetylated histones H3 and H4 more efficiently than hyperacetylated Proteinhistones H3 and H4.
0.9985	Methylation	We also show that hSWI / SNF - associated PRMT5 can Acetylationmethylate hypoacetylated histones H3 and ProteinH4 more efficiently than hyperacetylated histones H3 and H4.
0.9921	Methylation	We also show that hSWI / SNF - associated PRMT5 can Acetylationmethylate hypoacetylated Proteinhistones H3 and H4 more efficiently than hyperacetylated histones H3 and H4.
0.9894	Methylation	We also show that hSWI / SNF - associated PRMT5 can methylate Acetylationhypoacetylated histones H3 and ProteinH4 more efficiently than hyperacetylated histones H3 and H4.
0.9736	Methylation	We also show that hSWI / SNF - associated ProteinPRMT5 can Acetylationmethylate hypoacetylated histones H3 and H4 more efficiently than hyperacetylated histones H3 and H4.
0.9462	Methylation	We also show that hSWI / SNF - associated PRMT5 can methylate Acetylationhypoacetylated Proteinhistones H3 and H4 more efficiently than hyperacetylated histones H3 and H4.
0.5937	Methylation	We also show that hSWI / SNF - associated PRMT5 can Acetylationmethylate hypoacetylated histones H3 and H4 more efficiently than hyperacetylated histones H3 and ProteinH4 .
0.4155	Methylation	We also show that hSWI / SNF - associated PRMT5 can Acetylationmethylate hypoacetylated histones H3 and H4 more efficiently than hyperacetylated Proteinhistones H3 and H4.
	Methylation	We also show that hSWI / SNF - associated PRMT5 can methylate Deacetylationhypoacetylated histones H3 and ProteinH4 more efficiently than hyperacetylated histones H3 and H4.
	Methylation	We also show that hSWI / SNF - associated PRMT5 can methylate Deacetylationhypoacetylated Proteinhistones H3 and H4 more efficiently than hyperacetylated histones H3 and H4.

(1134)

Protein - protein interaction studies indicate that PRMT5 and mSin3A interact with the same hSWI / SNF subunits as those targeted by c - Myc.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1135)

These observations prompted us to examine the expression profile of the c - Myc target genes, carbamoyl - phosphate synthase - aspartate carbamoyltransferase - dihydroorotase ( cad ) and nucleolin ( nuc ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1136)

We found that cad repression is altered in cells that express inactive Brg1 and in cells treated with the HDAC inhibitor depsipeptide.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1137)

Using chromatin immunoprecipitation assays, we found that Brg1, mSin3A, HDAC2, and PRMT5 are directly recruited to the cad promoter.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1138)

These results suggest that hSWI / SNF complexes, through their ability to interact with activator and repressor proteins, control expression of genes involved in cell growth and proliferation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1139)

Centromere silencing and function in fission yeast is governed by the amino terminus of histone H3.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1140)

BACKGROUND : Centromeric domains often consist of repetitive elements that are assembled in specialized chromatin, characterized by hypoacetylation of histones H3 and H4 and methylation of lysine 9 of histone H3 ( K9 - MeH3 ).

Precision: 75.00

Recall: 100.00

F1: 85.71

Score	Relation	Text (Head - Tail)
0.9996	Methylation	BACKGROUND : Centromeric domains often consist of repetitive elements that are assembled in specialized chromatin, characterized by Acetylationhypoacetylation of Proteinhistones H3 and H4 and methylation of lysine 9 of histone H3 ( K9 - MeH3 ).
0.9993	Methylation	BACKGROUND : Centromeric domains often consist of repetitive elements that are assembled in specialized chromatin, characterized by hypoacetylation of histones H3 and H4 and Methylationmethylation of Entitylysine 9 of histone H3 ( K9 - MeH3 ).
0.9941	Methylation	BACKGROUND : Centromeric domains often consist of repetitive elements that are assembled in specialized chromatin, characterized by Acetylationhypoacetylation of histones H3 and ProteinH4 and methylation of lysine 9 of histone H3 ( K9 - MeH3 ).
0.9978	Methylation	BACKGROUND : Centromeric domains often consist of repetitive elements that are assembled in specialized chromatin, characterized by hypoacetylation of histones H3 and H4 and Methylationmethylation of Entitylysine 9 of histone H3 ( K9 - MeH3 ).

(1141)

Perturbation of this underacetylated state by transient treatment with histone deacetylase inhibitors leads to defective centromere function, correlating with delocalization of the heterochromatin protein Swi6 / HP1.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1142)

Likewise, deletion of the K9 - MeH3 methyltransferase Clr4 / Suvar39 causes defective chromosome segregation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1143)

Here, we create fission yeast strains retaining one histone H3 and H4 gene ; the creation of these strains allows mutation of specific N - terminal tail residues and their role in centromeric silencing and chromosome stability to be investigated.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1144)

RESULTS : Reduction of H3 / H4 gene dosage to one - third does not affect cell viability or heterochromatin formation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1145)

Mutation of lysines 9 or 14 or serine 10 within the amino terminus of histone H3 impairs centromere function, leading to defective chromosome segregation and Swi6 delocalization.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1146)

Surprisingly, silent centromeric chromatin does not require the conserved lysine 8 and 16 residues of histone H4.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1147)

CONCLUSIONS : To date, mutation of conserved N - terminal residues in endogenous histone genes has only been performed in budding yeast, which lacks the Clr4 / Suvar39 histone methyltransferase and Swi6 / HP1.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1148)

We demonstrate the importance of conserved residues within the histone H3 N terminus for the maintenance of centromeric heterochromatin in fission yeast.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1149)

In sharp contrast, mutation of two conserved lysines within the histone H4 tail has no impact on the integrity of centromeric heterochromatin.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1150)

Our data highlight the striking divergence between the histone tail requirements for the fission yeast and budding yeast silencing pathways.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1151)

Thermal stability and aggregation of sulfolobus solfataricus beta - glycosidase are dependent upon the N - epsilon - methylation of specific lysyl residues : critical role of in vivo post - translational modifications.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Relation	Text (Head - Tail)
1.0000	Methylation	Thermal stability and aggregation of sulfolobus solfataricus beta - glycosidase are dependent upon the MethylationN - epsilon - methylation of specific Entitylysyl residues : critical role of in vivo post - translational modifications.
1.0000	Methylation	Thermal stability and aggregation of sulfolobus solfataricus beta - glycosidase are dependent upon the MethylationN - epsilon - methylation of specific Entitylysyl residues : critical role of in vivo post - translational modifications.

(1152)

Methylation in vivo is a post - translational modification observed in several organisms belonging to eucarya, bacteria, and archaea.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1153)

Although important implications of this modification have been demonstrated in several eucaryotes, its biological role in hyperthermophilic archaea is far from being understood.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1154)

The aim of this work is to clarify some effects of methylation on the properties of beta - glycosidase from Sulfolobus solfataricus, by a structural comparison between the native, methylated protein and its unmethylated counterpart, recombinantly expressed in Escherichia coli.

Precision: 66.67

Recall: 100.00

F1: 80.00

Score	Relation	Text (Head - Tail)
0.9995	Methylation	The aim of this work is to clarify some effects of methylation on the properties of Proteinbeta - glycosidase from Sulfolobus solfataricus, by a structural comparison between the native, Methylationmethylated protein and its unmethylated counterpart, recombinantly expressed in Escherichia coli.
0.9989	Methylation	The aim of this work is to clarify some effects of methylation on the properties of Proteinbeta - glycosidase from Sulfolobus solfataricus, by a structural comparison between the native, methylated protein and its Methylationunmethylated counterpart, recombinantly expressed in Escherichia coli.
1.0000	Methylation	The aim of this work is to clarify some effects of Methylationmethylation on the properties of Proteinbeta - glycosidase from Sulfolobus solfataricus, by a structural comparison between the native, methylated protein and its unmethylated counterpart, recombinantly expressed in Escherichia coli.

(1155)

Analysis by Fourier transform infrared spectroscopy indicated similar secondary structure contents for the two forms of the protein.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1156)

However, the study of temperature perturbation by Fourier transform infrared spectroscopy and turbidimetry evidenced denaturation and aggregation events more pronounced in recombinant than in native beta - glycosidase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1157)

Red Nile fluorescence analysis revealed significant differences of surface hydrophobicity between the two forms of the protein.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1158)

Unlike the native enzyme, which dissociated into SDS - resistant dimers upon exposure to the detergent, the recombinant enzyme partially dissociated into monomers.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1159)

By electrospray mapping, the methylation sites of the native protein were identified.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1160)

A computational analysis of beta - glycosidase three - dimensional structure and comparisons with other proteins from S. solfataricus revealed analogies in the localization of methylation sites in terms of secondary structural elements and overall topology.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1161)

These observations suggest a role for the methylation of lysyl residues, located in selected domains, in the thermal stabilization of beta - glycosidase from S. solfataricus.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Relation	Text (Head - Tail)
1.0000	Methylation	These observations suggest a role for the Methylationmethylation of Entitylysyl residues , located in selected domains, in the thermal stabilization of beta - glycosidase from S. solfataricus.
0.9999	Methylation	These observations suggest a role for the Methylationmethylation of Entitylysyl residues, located in selected domains, in the thermal stabilization of beta - glycosidase from S. solfataricus.

(1162)

Pathways for phosphatidylcholine biosynthesis in bacteria.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1163)

Phosphatidylcholine ( PC ) is the major membrane - forming phospholipid in eukaryotes with important structural and signalling functions.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1164)

Although many prokaryotes lack PC, it can be found in significant amounts in membranes of rather diverse bacteria.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1165)

Two pathways for PC biosynthesis are known in bacteria, the methylation pathway and the phosphatidylcholine synthase ( PCS ) pathway.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1166)

In the methylation pathway, phosphatidylethanolamine is methylated three times to yield PC, in reactions catalysed by one or several phospholipid N - methyltransferases ( PMTs ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1167)

In the PCS pathway, choline is condensed directly with CDP - diacylglyceride to form PC in a reaction catalysed by PCS.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1168)

Using cell - free extracts, it was demonstrated that Sinorhizobium meliloti, Agrobacterium tumefaciens, Rhizobium leguminosarum, Bradyrhizobium japonicum, Mesorhizobium loti and Legionella pneumophila have both PMT and PCS activities.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1169)

In addition, Rhodobacter sphaeroides has PMT activity and Brucella melitensis, Pseudomonas aeruginosa and Borrelia burgdorferi have PCS activities.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1170)

Genes from M. loti and L. pneumophila encoding a Pmt or a Pcs activity and the genes from P. aeruginosa and Borrelia burgdorferi responsible for Pcs activity have been identified.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1171)

Based on these functional assignments and on genomic data, one might predict that if bacteria contain PC as a membrane lipid, they usually possess both bacterial pathways for PC biosynthesis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1172)

However, important pathogens such as Brucella melitensis, P. aeruginosa and Borrelia burgdorferi seem to be exceptional as they possess only the PCS pathway for PC formation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1173)

Rapid screening for S - adenosylmethionine - dependent methylation products by enzyme - transferred isotope patterns analysis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1174)

We report here an isotopic labeling and mass spectrometric method to rapidly identify S - adenosylmethionine ( AdoMet ) - dependent methylation products.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1175)

In the presence of CH ( 3 ) - and CD ( 3 ) - labeled AdoMet, a methyl transfer product appears as a doublet separated by 3 Da in a mass spectrum, while other compounds show their normal isotopic distribution.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1176)

Based on this unique isotopic pattern, methylation product ( s ) can be easily detected even from a mixture of cellular components.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1177)

To validate our method, the product of human thiopurine methyltransferase ( TPMT, EC 2. 1. 1. 67 ) has been successfully identified from both an in vitro assay and a whole - cell assay.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1178)

This method is generally applicable to AdoMet - dependent transmethylation and other group - transfer reactions, and constitutes the first example of a general strategy of enzyme - transferred isotope patterns ( ETIPs ) analysis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1179)

Subunit - specific sulphation of oligosaccharides relating to charge - heterogeneity in porcine lutrophin isoforms.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1180)

Lutrophin ( LH ) consists of an array of isoforms with different charges and bioactivities.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1181)

This study was undertaken to clarify specifically how oligosaccharides of alpha and beta subunits contribute to LH isoform charges.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1182)

Porcine LH ( pLH ) was separated into four isoforms by isoelectric focusing ( IEF ), followed by subunit isolation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1183)

Their oligosaccharides were released by hydrazinolysis, labelled by reduction with NaB3H4, and fractionated by HPLC with a Mono Q column into five populations differing in the number of sulphate ( S ) and sialic acid ( N ) residues, designated as Neutral, N - 1, S - 1, S - N and S - 2.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1184)

Oligosaccharides were predominantly sulphated ( S - 1 and S - 2 ) and infrequently sialylated ( N - 1 and S - N ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1185)

Further analysis, including concanavalin A ( Con A ) affinity chromatography, desialylation, desulphation, sequential exoglycosidase digestion and methylation, clarified the structures of the acidic oligosaccharides.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1186)

All were of the biantennary complex type.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1187)

Their two peripheral branches were SO4 - 4GalNAc beta 1 - 4Glc - NAc and GalNAc beta 1 - 4GlcNAc or GlcNAc in S - 1, SO4 - 4GalNAc beta 1 - 4GlcNAc and Sia alpha 2 - 6Gal beta 1 - 4GlcNAc in S - N, and ( SO4 - 4GalNAc beta 1 - 4GlcNAc ) 2 in S - 2 ( where GalNAc is N - acetylgalactosamine and GlcNAc is N - acetylglucosamine ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1188)

Ten percent of S - 1 and of S - N had a bisecting GlcNAc residue.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1189)

Sulphate residues occurred in nearly the same amount for both subunits ; however, the alpha and beta subunits were sulphated differently.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1190)

S - 1 predominated in the alpha subunit, while S - 1 and S - 2 were major components in the beta subunit. ( ABSTRACT TRUNCATED AT 250 WORDS )

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1191)

The MTHFR 677C > T polymorphism is associated with an increased risk of hepatocellular carcinoma in patients with alcoholic cirrhosis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1192)

Methylenetetrahydrofolate reductase ( MTHFR ), a key enzyme in folate metabolism, plays a major role in the provision of methyl groups for DNA methylation and in the production of dTMP for DNA synthesis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1193)

Different polymorphisms have been described for this enzyme, the most studied being the C677T, which has been shown to be associated with predisposition to colorectal cancer in patients who consume a high alcohol diet.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1194)

The aim of this study was to determine whether the MTHFR polymorphism is related to hepatocellular carcinoma ( HCC ) in patients with alcoholic cirrhosis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1195)

MTHFR genotypes were determined in 300 liver transplant patients, 72 of whom had alcoholic cirrhosis with HCC and 122 of whom had alcoholic cirrhosis without HCC.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1196)

The remaining patients were transplanted for HCC on normal liver ( n = 27 ) or viral cirrhosis with HCC ( n = 49 ) or without HCC ( n = 30 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1197)

We also tested 80 healthy subjects.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1198)

Among the group of patients transplanted for alcoholic cirrhosis, the frequency of MTHFR variants CC versus CT and TT was significantly higher in patients with HCC than in patients without macroscopic evidence of HCC ( P = 0. 02 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1199)

This difference was not observed between patients with and without HCC developed either on viral cirrhosis or on non - cirrhotic liver.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1200)

If we considered all the patients transplanted for HCC, the MTHFR CC genotype was significantly higher in patients who had developed HCC on alcoholic cirrhosis rather than on viral cirrhosis ( P = 0. 002 ) or on non - cirrhotic livers ( P = 0. 02 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1201)

The relative risk for HCC in subjects with alcoholic cirrhosis and the CC genotype was 2. 03.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1202)

These results suggest that the MTHFR CC genotype increases the risk to develop HCC in patients who consume a high alcohol diet.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1203)

Mbd1 is recruited to both methylated and nonmethylated CpGs via distinct DNA binding domains.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1204)

MBD1 is a vertebrate methyl - CpG binding domain protein ( MBD ) that can bring about repression of methylated promoter DNA sequences.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1205)

Like other MBD proteins, MBD1 localizes to nuclear foci that in mice are rich in methyl - CpG.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1206)

In methyl - CpG - deficient mouse cells, however, Mbd1 remains localized to heterochromatic foci whereas other MBD proteins become dispersed in the nucleus.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1207)

We find that Mbd1a, a major mouse isoform, contains a CXXC domain ( CXXC - 3 ) that binds specifically to nonmethylated CpG, suggesting an explanation for methylation - independent localization.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1208)

Transfection studies demonstrate that the CXXC - 3 domain indeed targets nonmethylated CpG sites in vivo.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1209)

Repression of nonmethylated reporter genes depends on the CXXC - 3 domain, whereas repression of methylated reporters requires the MBD.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1210)

Our findings indicate that MBD1 can interpret the CpG dinucleotide as a repressive signal in vivo regardless of its methylation status.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1211)

Recombinant human zona pellucida proteins ZP1, ZP2 and ZP3 co - expressed in a human cell line.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1212)

AIM : To produce biologically active recombinant human ( rh ) ZP proteins in a human cell for use in sperm function tests.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1213)

METHODS : The human embryonic kidney cell line 293T was employed to produce rhZP1, rhZP2 and rhZP3 proteins individually and together by co - expression.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1214)

Presence of these proteins in the culture medium and cell lysate was assessed by Western blotting analysis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1215)

The effect of the recombinant proteins on the human AR was assessed.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1216)

RESULTS : RhZP2 and rhZP3 were secreted into the culture medium, whereas rhZP1 was found only in the cell lysate.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1217)

Interestingly, when all zona pellucida proteins were co - expressed in the same cells, rhZP1 was also secreted into the culture medium.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1218)

However, despite the presence of all three ZP proteins in sufficient concentration and evidence of heavy glycosylation on gel electrophoresis, biological activity to induce the AR was not observed.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1219)

CONCLUSION : RhZP1, rhZP2 and rhZP3 were successfully expressed in the human embryonic kidney cell line 293T.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1220)

It appears that an interaction amongst these proteins may be required for release of rhZP1 from the cell.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1221)

Although this approach is not satisfactory for producing active human ZP proteins, it makes a significant contribution to the understanding of the structural and functional characteristics of the ZP proteins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1222)

Leu - 574 of human HIF - 1alpha is a molecular determinant of prolyl hydroxylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1223)

Hypoxia - inducible factor ( HIF ) - 1alpha, a master regulator of oxygen homeostasis, regulates genes crucial for cell growth and survival.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1224)

In normoxia, HIF - 1alpha is constantly degraded via the ubiquitin - proteasome pathway.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1225)

The von Hippel - Lindau ( VHL ) E3 ubiquitin ligase binds HIF - 1alpha through specific recognition of hydroxylated Pro - 402 or Pro - 564, both of which are modified by the oxygen - dependent HIF prolyl hydroxylases ( PHDs / HPHs ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9956	Methylation	The von Hippel - Lindau ( VHL ) E3 ubiquitin ligase binds HIF - 1alpha through specific recognition of Hydroxylationhydroxylated Pro - 402 or EntityPro - 564 , both of which are modified by the oxygen - dependent HIF prolyl hydroxylases ( PHDs / HPHs ).
0.9918	Methylation	The von Hippel - Lindau ( VHL ) E3 ubiquitin ligase binds HIF - 1alpha through specific recognition of Hydroxylationhydroxylated EntityPro - 402 or Pro - 564, both of which are modified by the oxygen - dependent HIF prolyl hydroxylases ( PHDs / HPHs ).

(1226)

Despite the identification of a conserved Leu - X - X - Leu - Ala - Pro motif, the molecular requirement of HIF - 1alpha for PHDs / HPHs binding remains elusive.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1227)

Recently, we demonstrated that Leu - 574 of human HIF - 1alpha - - 10 residues downstream of Pro - 564 - - is essential for VHL recognition.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1228)

We show here that the role of Leu - 574 is to recruit PHD2 / HPH2 for Pro - 564 hydroxylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9971	Methylation	We show here that the role of Leu - 574 is to recruit ProteinPHD2 / HPH2 for Pro - 564 Hydroxylationhydroxylation .
0.9956	Methylation	We show here that the role of Leu - 574 is to recruit PHD2 / HPH2 for EntityPro - 564 Hydroxylationhydroxylation .

(1229)

An antibody specific for hydroxylated Pro - 564 has been used to determine the hydroxylation status ; mutation or deletion of Leu - 574 results in a significant decrease in the ratio of the hydroxylated HIF - 1alpha to the total amount.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9957	Methylation	An antibody specific for Hydroxylationhydroxylated EntityPro - 564 has been used to determine the hydroxylation status ; mutation or deletion of Leu - 574 results in a significant decrease in the ratio of the hydroxylated HIF - 1alpha to the total amount.
0.9937	Methylation	An antibody specific for hydroxylated Pro - 564 has been used to determine the hydroxylation status ; mutation or deletion of Leu - 574 results in a significant decrease in the ratio of the Hydroxylationhydroxylated ProteinHIF - 1alpha to the total amount.

(1230)

The nine - residue spacing between Pro - 564 and Leu - 574 is not obligatory for prolyl hydroxylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9969	Methylation	The nine - residue spacing between Pro - 564 and Leu - 574 is not obligatory for Entityprolyl Hydroxylationhydroxylation .

(1231)

Furthermore, mutation of Leu - 574 disrupts the binding of PHD2 / HPH2, a key prolyl hydroxylase for oxygen - dependent proteolysis of HIF - 1alpha.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1232)

Hence, our findings indicate that Leu - 574 is essential for recruiting PHD2 / HPH2, thereby providing a molecular basis for modulating HIF - 1alpha activity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1233)

Transferrin microheterogeneity in fetal blood.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1234)

OBJECTIVES : To investigate the distribution of microheterogeneous subfractions of transferrin in fetal blood and the influence of highly sialylated transferrins on fetal growth.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1235)

STUDY METHOD : Serum transferrin concentrations were determined by a standard turbidimetric assay.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1236)

Microheterogeneous transferrin subfractions were assessed by crossed immunoisoelectric focusing.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1237)

RESULTS : In normal term infants, total serum transferrin concentrations and percent distribution of highly sialylated transferrins ( > or = 5 - sialo - transferrins ) were markedly lower ; the percent distributions of hyposialylated transferrins ( 0 - and 1 - sialo - transferrins ) were apparently higher than those in non - pregnant and pregnant women.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1238)

There was no significant positive correlation between the serum concentrations of total transferrin or highly sialylated transferrins in infants'blood and birth weights ( r = 0. 187, p = 0. 582 ; r = 0. 374, p = 0. 257, respectively ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1239)

CONCLUSION : The transferrin microheterogeneity pattern shifted towards reduced glycosylation and sialylation in addition to a decrease in total transferrin concentration in fetal blood compared to that of non - pregnant and pregnant women.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9978	Methylation	CONCLUSION : The Proteintransferrin microheterogeneity pattern shifted towards reduced Glycosylationglycosylation and sialylation in addition to a decrease in total transferrin concentration in fetal blood compared to that of non - pregnant and pregnant women.

(1240)

The concentrations of serum total transferrin and the highly sialylated transferrins in fetal blood, if higher than a certain level, did not seem to have any influence on normal fetal growth.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1241)

Heterochromatin and tri - methylated lysine 20 of histone H4 in animals.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9993	Methylation	Heterochromatin and Methylationtri - methylated Entitylysine 20 of histone H4 in animals.

(1242)

Tri - methylated lysine 20 on histone H4 ( Me ( 3 ) K20H4 ) is a marker of constitutive heterochromatin in murine interphase and metaphase cells.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Relation	Text (Head - Tail)
0.9989	Methylation	MethylationTri - methylated Entitylysine 20 on histone H4 ( Me ( 3 ) K20H4 ) is a marker of constitutive heterochromatin in murine interphase and metaphase cells.
0.9968	Methylation	MethylationTri - methylated Entitylysine 20 on histone H4 ( Me ( 3 ) K20H4 ) is a marker of constitutive heterochromatin in murine interphase and metaphase cells.

(1243)

Heterochromatin marked by Me ( 3 ) K20H4 replicates late during S phase of the cell cycle.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1244)

Serum starvation increases the number of cells that exhibit high levels of Me ( 3 ) K20H4 at constitutive heterochromatin.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1245)

Me ( 3 ) K20H4 is also present at the centromeric heterochromatin of most meiotic chromosomes during spermatogenesis and at the pseudoautosomal region, as well as at some telomeres.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1246)

It is not present on the XY - body.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1247)

During murine embryogenesis the maternal pronucleus contains Me ( 3 ) K20H4 ; Me ( 3 ) K20H4 is absent from the paternal pronucleus.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1248)

On Drosophila polytene chromosomes Me ( 3 ) K20H4 is present in a'punctate pattern'at many chromosomal bands, including the chromocenter.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1249)

In coccids it is present on the facultatively heterochromatinised paternal chromosome set.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1250)

We also present evidence that Me ( 3 ) K20H4 is dependent upon H3 - specific Suv ( 3 ) 9 histone methyltransferase activity, suggesting that there may be'epigenetic cross - talk'between histones H3 and H4.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1251)

The histone modification pattern of active genes revealed through genome - wide chromatin analysis of a higher eukaryote.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1252)

The covalent modification of nucleosomal histones has emerged as a major determinant of chromatin structure and gene activity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1253)

To understand the interplay between various histone modifications, including acetylation and methylation, we performed a genome - wide chromatin structure analysis in a higher eukaryote.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	To understand the interplay between various Proteinhistone modifications, including Acetylationacetylation and methylation, we performed a genome - wide chromatin structure analysis in a higher eukaryote.
0.9990	Methylation	To understand the interplay between various Proteinhistone modifications, including acetylation and Methylationmethylation , we performed a genome - wide chromatin structure analysis in a higher eukaryote.

(1254)

We found a binary pattern of histone modifications among euchromatic genes, with active genes being hyperacetylated for H3 and H4 and hypermethylated at Lys 4 and Lys 79 of H3, and inactive genes being hypomethylated and deacetylated at the same residues.

Precision: 61.54

Recall: 100.00

F1: 76.19

Score	Relation	Text (Head - Tail)
1.0000	Methylation	We found a binary pattern of histone modifications among euchromatic genes, with active genes being Acetylationhyperacetylated for ProteinH3 and H4 and hypermethylated at Lys 4 and Lys 79 of H3, and inactive genes being hypomethylated and deacetylated at the same residues.
0.9997	Methylation	We found a binary pattern of histone modifications among euchromatic genes, with active genes being hyperacetylated for H3 and H4 and Methylationhypermethylated at EntityLys 4 and Lys 79 of H3, and inactive genes being hypomethylated and deacetylated at the same residues.
0.9991	Methylation	We found a binary pattern of histone modifications among euchromatic genes, with active genes being Acetylationhyperacetylated for H3 and ProteinH4 and hypermethylated at Lys 4 and Lys 79 of H3, and inactive genes being hypomethylated and deacetylated at the same residues.
0.9828	Methylation	We found a binary pattern of histone modifications among euchromatic genes, with active genes being hyperacetylated for H3 and H4 and Methylationhypermethylated at Lys 4 and EntityLys 79 of H3, and inactive genes being hypomethylated and deacetylated at the same residues.
0.8726	Methylation	We found a binary pattern of histone modifications among euchromatic genes, with active genes being hyperacetylated for H3 and H4 and hypermethylated at EntityLys 4 and Lys 79 of H3, and inactive genes being Methylationhypomethylated and deacetylated at the same residues.
0.8613	Methylation	We found a binary pattern of histone modifications among euchromatic genes, with active genes being hyperacetylated for H3 and H4 and hypermethylated at Lys 4 and EntityLys 79 of H3, and inactive genes being Methylationhypomethylated and deacetylated at the same residues.
0.7035	Methylation	We found a binary pattern of histone modifications among euchromatic genes, with active genes being hyperacetylated for H3 and ProteinH4 and hypermethylated at Lys 4 and Lys 79 of H3, and inactive genes being hypomethylated and Deacetylationdeacetylated at the same residues.
0.4697	Methylation	We found a binary pattern of histone modifications among euchromatic genes, with active genes being hyperacetylated for ProteinH3 and H4 and hypermethylated at Lys 4 and Lys 79 of H3, and inactive genes being hypomethylated and Deacetylationdeacetylated at the same residues.
0.9985	Methylation	We found a binary pattern of histone modifications among euchromatic genes, with active genes being hyperacetylated for H3 and H4 and Methylationhypermethylated at EntityLys 4 and Lys 79 of H3, and inactive genes being hypomethylated and deacetylated at the same residues.
0.9500	Methylation	We found a binary pattern of histone modifications among euchromatic genes, with active genes being hyperacetylated for H3 and H4 and Methylationhypermethylated at Lys 4 and EntityLys 79 of H3, and inactive genes being hypomethylated and deacetylated at the same residues.
0.6089	Methylation	We found a binary pattern of histone modifications among euchromatic genes, with active genes being hyperacetylated for H3 and H4 and hypermethylated at Lys 4 and EntityLys 79 of H3, and inactive genes being Methylationhypomethylated and deacetylated at the same residues.
0.5887	Methylation	We found a binary pattern of histone modifications among euchromatic genes, with active genes being hyperacetylated for H3 and H4 and hypermethylated at EntityLys 4 and Lys 79 of H3, and inactive genes being Methylationhypomethylated and deacetylated at the same residues.
0.4885	Methylation	We found a binary pattern of histone modifications among euchromatic genes, with active genes being hyperacetylated for H3 and ProteinH4 and hypermethylated at Lys 4 and Lys 79 of H3, and inactive genes being Methylationhypomethylated and deacetylated at the same residues.

(1255)

Furthermore, the degree of modification correlates with the level of transcription, and modifications are largely restricted to transcribed regions, suggesting that their regulation is tightly linked to polymerase activity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1256)

Less frequent promoter hypermethylation of DLC - 1 gene in primary breast cancers.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9958	Methylation	Less frequent Entitypromoter DNA_methylationhypermethylation of DLC - 1 gene in primary breast cancers.

(1257)

Absence or low expression of DLC - 1, a tumor suppressor gene, in breast cancers has been shown recently.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1258)

LOH of 8p12 - p22, on which DLC - 1 is located, is frequent in breast cancers, but the correlation between low expression of DLC - 1 and LOH has not been confirmed.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1259)

To determine the implication of aberrant methylation, one of the most frequent mechanisms of silencing the tumor suppressor or cancer - related genes, we examined the methylation status of DLC - 1 promoter region in breast cancer cell lines and primary breast tumors.

Precision: 20.00

Recall: 100.00

F1: 33.33

Score	Relation	Text (Head - Tail)
0.9973	Methylation	To determine the implication of aberrant methylation, one of the most frequent mechanisms of silencing the tumor suppressor or cancer - related genes, we examined the DNA_methylationmethylation status of DLC - 1 Entitypromoter region in breast cancer cell lines and primary breast tumors.
0.9950	Methylation	To determine the implication of aberrant methylation, one of the most frequent mechanisms of silencing the tumor suppressor or cancer - related genes, we examined the DNA_methylationmethylation status of DLC - 1 promoter Entityregion in breast cancer cell lines and primary breast tumors.
0.9946	Methylation	To determine the implication of aberrant methylation, one of the most frequent mechanisms of silencing the tumor suppressor or cancer - related genes, we examined the DNA_methylationmethylation status of DLC - 1 Entitypromoter region in breast cancer cell lines and primary breast tumors.
0.4963	Methylation	To determine the implication of aberrant DNA_methylationmethylation , one of the most frequent mechanisms of silencing the tumor suppressor or cancer - related genes, we examined the methylation status of DLC - 1 promoter Entityregion in breast cancer cell lines and primary breast tumors.
0.4678	Methylation	To determine the implication of aberrant DNA_methylationmethylation , one of the most frequent mechanisms of silencing the tumor suppressor or cancer - related genes, we examined the methylation status of DLC - 1 Entitypromoter region in breast cancer cell lines and primary breast tumors.

(1260)

The hypermethylation status was examined by MSP and 25 % of cell lines harbored a methylated allele.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1261)

The gene silencing by methylation was also confirmed by the re - expression of DLC - 1 by the 5 - aza - 2'- deoxycytidine treatment in DLC - 1 hypermethylated cell line.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9992	Methylation	The gene silencing by methylation was also confirmed by the re - expression of DLC - 1 by the 5 - aza - 2'- deoxycytidine treatment in ProteinDLC - 1 DNA_methylationhypermethylated cell line.

(1262)

But the methylation of DLC - 1 gene was less frequently shown in primary breast cancers ( 10 % ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	But the DNA_methylationmethylation of ProteinDLC - 1 gene was less frequently shown in primary breast cancers ( 10 % ).

(1263)

These data suggest that hypermethylation is responsible for silencing of DLC - 1 gene in a limited portion of breast cancers.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	These data suggest that DNA_methylationhypermethylation is responsible for silencing of ProteinDLC - 1 gene in a limited portion of breast cancers.

(1264)

Glycosylation of the ENV spike of primate immunodeficiency viruses and antibody neutralization.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	GlycosylationGlycosylation of the ProteinENV spike of primate immunodeficiency viruses and antibody neutralization.

(1265)

Neutralizing antibody titers have been correlated with protection following vaccination against many viral pathogens.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1266)

The logical target of protective antibody responses elicited by potential HIV vaccines should be the viral Env spike on the surface of the virion.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1267)

However, the potency and titers of neutralizing antibodies that arise during HIV infection are generally discouragingly low and the antibodies that do arise recognize mainly autologous virus.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1268)

This is thought to be a result of a combination of immunodominance of hypervariable regions of the Env protein that can easily escape neutralization, antibody reactivity to gp160 " decoy " protein in cell surface debris or monomeric gp120, conformational constraints within the Env trimer that create unfavorable antibody binding conditions and extensive glycosylation of the exposed regions of Env within the trimer.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9998	Methylation	This is thought to be a result of a combination of immunodominance of hypervariable regions of the Env protein that can easily escape neutralization, antibody reactivity to gp160 " decoy " protein in cell surface debris or monomeric gp120, conformational constraints within the Env trimer that create unfavorable antibody binding conditions and extensive Glycosylationglycosylation of the exposed regions of ProteinEnv within the trimer.

(1269)

This review will describe current knowledge regarding glycosylation as a mechanism of neutralization resistance and discuss experimental approaches used to overcome this resistance.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1270)

Part of the strategy toward development of an optimally immunogenic Env spike will likely require modification of Env glycosylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9985	Methylation	Part of the strategy toward development of an optimally immunogenic Env spike will likely require modification of ProteinEnv Glycosylationglycosylation .

(1271)

Comparison of methylation - specific polymerase chain reaction ( MSP ) with reverse transcriptase - polymerase chain reaction ( RT - PCR ) in peripheral blood of gastric cancer patients.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1272)

BACKGROUND AND OBJECTIVES : To achieve a complete cure in gastric cancer, primary and recurrent tumors must be detected at an early stage.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1273)

This study was designed to compare carcinoembryonic antigen ( CEA ) - specific reverse transcriptase - polymerase chain reaction ( RT - PCR ) and methylation - specific polymerase chain reaction ( MSP ) for p16, E - cadherin, and retinoic acid receptor beta ( RARbeta ) genes using blood samples from gastric cancer patients.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1274)

METHODS : Preoperative blood samples obtained from 41 patients with gastric cancer, including 9 with early - stage disease, and were subjected to RT - PCR and MSP assays.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1275)

RESULTS : Ten of 41 ( 24 % ) patients exhibited a CEA - specific signal by RT - PCR.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1276)

Positive rates were 11, 13, 50, and 50 % in stages I, II, III, and IV, respectively.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1277)

A significant association was found between RT - PCR results and stage ( P < 0. 01 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1278)

The MSP assay detected hypermethylation of p16 in 9 patients ( 22 % ), E - cadherin in 9 patients ( 22 % ), and RARbeta in 6 patients ( 15 % ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9994	Methylation	The MSP assay detected DNA_methylationhypermethylation of p16 in 9 patients ( 22 % ), ProteinE - cadherin in 9 patients ( 22 % ), and RARbeta in 6 patients ( 15 % ).
0.9993	Methylation	The MSP assay detected DNA_methylationhypermethylation of Proteinp16 in 9 patients ( 22 % ), E - cadherin in 9 patients ( 22 % ), and RARbeta in 6 patients ( 15 % ).
0.9988	Methylation	The MSP assay detected DNA_methylationhypermethylation of p16 in 9 patients ( 22 % ), E - cadherin in 9 patients ( 22 % ), and ProteinRARbeta in 6 patients ( 15 % ).

(1279)

Altogether, 18 patients ( 44 % ) showed hypermethylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1280)

The positive rates were 37, 50, 40, and 75 % in stages I, II, III, and IV, respectively.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1281)

A significant association was found between aberrant methylation and venous invasion ( P < 0. 05 ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1282)

Neither the CEA - specific signal nor hypermethylation was detected in serum from control volunteers.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1283)

CONCLUSIONS : The detection rate of MSP was higher than that of RT - PCR in gastric cancer.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1284)

Both assays can serve as markers that allow selection of those cases requiring more intensive screening and aggressive postoperative treatment.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1285)

Superoxide dismutase and catalase are required to detect (. - ) NO from both coupled and uncoupled neuronal no synthase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1286)

Despite numerous approaches to measuring nitric oxide ( (. - ) NO ) formation from purified NO synthase ( NOS ), it is still not clear whether (. - ) NO is a direct or indirect product of the NO synthase reaction.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1287)

The direct detection of catalytically formed (. - ) NO is complicated by side reactions with reactive oxide species like H ( 2 ) O ( 2 ) and superoxide.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1288)

The aim of the present study was therefore to reinvestigate these reactions both electrochemically and by chemiluminescence detection with particular emphasis on the requirement for cofactors and their interference with (. - ) NO detection.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1289)

Flavins were found to generate large amounts of H ( 2 ) O ( 2 ) and were therefore excluded from subsequent incubations.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1290)

Under conditions of both coupled and uncoupled catalysis, SOD was absolutely required to detect (. - ) NO from NOS.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1291)

H ( 2 ) O ( 2 ) formation took place also in the presence of SOD and gave a smaller yet significant interfering signal.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1292)

Similar data were obtained when the proposed intermediate N ( omega ) - hydroxy - l - arginine was utilized as substrate.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1293)

In conclusion, standard Clark - type ( ) NO electrodes are cross - sensitive to H ( 2 ) O ( 2 ) and therefore both SOD and catalase are absolutely required to specifically detect (. - ) NO from NOS.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1294)

Widespread activation of antibiotic biosynthesis by S - adenosylmethionine in streptomycetes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1295)

The effect of S - adenosylmethionine ( SAM ) on the production of various antibiotics was investigated to determine whether SAM - dependent methylation is required in biosynthetic pathways of antibiotics.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1296)

Pristinamycin II ( B ) and granaticin do not require SAM - dependent methylation in their biosynthesis pathways, and production of these two antibiotics was increased about 2 - fold when a low concentration ( 50 and 10 microM, respectively ) of SAM was treated ; in contrast, oleandomycin and avermectin B1a require SAM as a methyl donor in their biosynthesis, and production of these two antibiotics was increased 5 - fold and 6 - fold, depending on the SAM concentration within a certain range.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1297)

We also found that the transcription of a pathway - specific regulator, gra - ORF9, was activated by exogenous SAM treatment.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1298)

Production of oleandomycin and avermectin B1a was decreased by using a methyltransferase inhibitor, sinefungin, but the production levels of these antibiotics were restored to the control level by simultaneously adding SAM and sinefungin.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1299)

Interestingly, we have found a similar stimulatory effect of S - adenosylhomocysteine ( SAH ), the methylation product of SAM, on antibiotic production in the four strains.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1300)

Our results clearly demonstrate the widespread activation of antibiotic production using SAM in streptomycetes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1301)

A phosphatidylserine - binding site in the cytosolic fragment of Clostridium sordellii lethal toxin facilitates glucosylation of membrane - bound Rac and is required for cytotoxicity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1302)

Large clostridial toxins glucosylate some small G proteins on a threonine residue, thereby preventing their interactions with effector molecules and regulators.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1303)

We show that the glucosyltransferase domain of lethal toxin from Clostridium sordellii ( LT ( cyt ) ; amino acids 1 - 546 ), which is released into the cytosol during cell infection, binds preferentially to liposomes containing phosphatidylserine as compared with other anionic lipids.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1304)

The binding of LT ( cyt ) to phosphatidylserine increases by two orders of magnitude the rate of glucosylation of liposome - bound geranyl - geranylated Rac - GDP.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1305)

Limited proteolysis and deletion studies show that the binding site for phosphatidylserine lies within the first 18 N - terminal residues of LT ( cyt ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1306)

Deletion of these residues abolishes the effect of phosphatidylserine on the activity of LT ( cyt ) on liposome - bound geranyl - geranylated Rac - GDP and prevents the morphological effects induced by LT ( cyt ) microinjection into various cells, but it does not affect the intrinsic activity of LT ( cyt ) on non - geranyl - geranylated Rac - GDP in solution.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1307)

We conclude that the avidity of LT ( cyt ) for phosphatidylserine facilitates its targeting to the cytosolic leaflet of cell membranes and, notably, the plasma membrane, where this anionic lipid is abundant and where several targets of lethal toxin reside.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1308)

Arginine residues as stabilizing elements in proteins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1309)

Site - specific substitutions of arginine for lysine in the thermostable D - xylose isomerase ( XI ) from Actinoplanes missouriensis are shown to impart significant heat stability enhancement in the presence of sugar substrates most probably by interfering with nonenzymatic glycation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1310)

The same substitutions are also found to increase heat stability in the absence of any sugar derivatives, where a mechanism based on prevention of glycation can no longer be invoked.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1311)

This rather conservative substitution is moreover shown to improve thermostability in two other structurally unrelated proteins, human copper, zinc - superoxide dismutase ( CuZnSOD ) and D - glyceraldehyde - 3 - phosphate dehydrogenase ( GAPDH ) from Bacillus subtilis.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1312)

The stabilizing effect of Lys - - - - Arg substitutions is rationalized on the basis of a detailed analysis of the crystal structures of wild - type XI and of engineered variants with Lys - - - - Arg substitution at four distinct locations, residues 253, 309, 319, and 323.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1313)

Molecular model building analysis of the structures of wild - type and mutant CuZnSOD ( K9R ) and GAPDH ( G281K and G281R ) is used to explain the observed stability enhancement in these proteins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1314)

In addition to demonstrating that even thermostable proteins can lend themselves to further stability improvement, our findings provide direct evidence that arginine residues are important stabilizing elements in proteins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1315)

Moreover, the stabilizing role of electrostatic interactions, particularly between subunits in oligomeric proteins, is documented.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1316)

Human SWI / SNF - associated PRMT5 methylates histone H3 arginine 8 and negatively regulates expression of ST7 and NM23 tumor suppressor genes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9965	Methylation	Human SWI / SNF - associated ProteinPRMT5 Methylationmethylates histone H3 arginine 8 and negatively regulates expression of ST7 and NM23 tumor suppressor genes.

(1317)

Protein arginine methyltransferases ( PRMTs ) have been implicated in transcriptional activation and repression, but their role in controlling cell growth and proliferation remains obscure.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1318)

We have recently shown that PRMT5 can interact with flag - tagged BRG1 - and hBRM - based hSWI / SNF chromatin remodelers and that both complexes can specifically methylate histones H3 and H4.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9992	Methylation	We have recently shown that PRMT5 can interact with flag - tagged BRG1 - and hBRM - based hSWI / SNF chromatin remodelers and that both complexes can specifically Methylationmethylate histones H3 and ProteinH4 .
0.9981	Methylation	We have recently shown that PRMT5 can interact with flag - tagged BRG1 - and hBRM - based hSWI / SNF chromatin remodelers and that both complexes can specifically Methylationmethylate Proteinhistones H3 and H4.

(1319)

Here we report that PRMT5 can be found in association with endogenous hSWI / SNF complexes, which can methylate H3 and H4 N - terminal tails, and show that H3 arginine 8 and H4 arginine 3 are preferred sites of methylation by recombinant and hSWI / SNF - associated PRMT5.

Precision: 60.00

Recall: 100.00

F1: 75.00

Score	Relation	Text (Head - Tail)
0.9996	Methylation	Here we report that PRMT5 can be found in association with endogenous hSWI / SNF complexes, which can methylate H3 and H4 N - terminal tails, and show that H3 arginine 8 and H4 arginine 3 are preferred sites of Methylationmethylation by recombinant and hSWI / SNF - associated ProteinPRMT5 .
0.9996	Methylation	Here we report that PRMT5 can be found in association with endogenous hSWI / SNF complexes, which can methylate H3 and H4 N - terminal tails, and show that H3 arginine 8 and H4 Entityarginine 3 are preferred sites of Methylationmethylation by recombinant and hSWI / SNF - associated PRMT5.
0.9957	Methylation	Here we report that PRMT5 can be found in association with endogenous hSWI / SNF complexes, which can methylate H3 and H4 N - terminal tails, and show that H3 Entityarginine 8 and H4 arginine 3 are preferred sites of Methylationmethylation by recombinant and hSWI / SNF - associated PRMT5.
0.9879	Methylation	Here we report that PRMT5 can be found in association with endogenous hSWI / SNF complexes, which can methylate H3 and H4 N - terminal tails, and show that H3 Entityarginine 8 and H4 arginine 3 are preferred sites of Methylationmethylation by recombinant and hSWI / SNF - associated PRMT5.
0.5024	Methylation	Here we report that PRMT5 can be found in association with endogenous hSWI / SNF complexes, which can Catalysismethylate H3 and H4 N - terminal tails, and show that H3 Entityarginine 8 and H4 arginine 3 are preferred sites of methylation by recombinant and hSWI / SNF - associated PRMT5.

(1320)

To elucidate the role played by PRMT5 in gene regulation, we have established a PRMT5 antisense cell line and determined by microarray analysis that more genes are derepressed when PRMT5 levels are reduced.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1321)

Among the affected genes, we show that suppressor of tumorigenicity 7 ( ST7 ) and nonmetastatic 23 ( NM23 ) are direct targets of PRMT5 - containing BRG1 and hBRM complexes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1322)

Furthermore, we demonstrate that expression of ST7 and NM23 is reduced in a cell line that overexpresses PRMT5 and that this decrease in expression correlates with H3R8 methylation, H3K9 deacetylation, and increased transformation of NIH 3T3 cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1323)

These findings suggest that the BRG1 - and hBRM - associated PRMT5 regulates cell growth and proliferation by controlling expression of genes involved in tumor suppression.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1324)

Sex - and tissue - specific expression of maintenance and de novo DNA methyltransferases upon low dose X - irradiation in mice.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1325)

DNA methylation is crucial for normal development, proliferation, and proper maintenance of genome stability for a given organism.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1326)

A variety of DNA damaging agents that are known to affect genome stability were also shown to alter DNA methylation patterns.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1327)

We have recently pioneered the studies in the area of the radiation effects on DNA methylation, and found that radiation exposure led to substantial dose - dependent and tissue - specific DNA hypomethylation, which was much more pronounced in spleen and liver of female animals.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1328)

The exact mechanisms of radiation - induced DNA hypomethylation are still to be uncovered.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1329)

We have previously shown that one of those mechanisms may potentially be DNA repair related.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1330)

Another possible mechanism may be linked to changes in the expression of DNA methyltransferases ( DNMTs ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1331)

In the current study, we examined the radiation - induced changes in expression of maintenance DNMT1, and de novo methyltransferases DNMT3a and DNMT3b in spleen and liver of irradiated animals.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1332)

This was paralleled by the studies of acute and chronic IR - induced methylation changes in spleen and liver of intact animals, as well as in animals with altered sex hormone status.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1333)

Here we report that radiation - induced DNA methylation changes correlated with radiation - induced alterations in expression of DNA methyltransferases.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1334)

We present the data on tissue - specificity in radiation - induced expression of DNA methyltransferases, and prove that changes in the expression of de novo methyltransferases DNMT3a and DNMT3b are the most important in radiation - induced DNA methylation alterations.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1335)

We also discuss the role of sex hormones, especially estrogen, in the generation of the sex - specific radiation - induced methylation changes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1336)

Characterization of a novel amylolytic enzyme encoded by a gene from a soil - derived metagenomic library.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1337)

It has been estimated that less than 1 % of the microorganisms in nature can be cultivated by conventional techniques.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1338)

Thus, the classical approach of isolating enzymes from pure cultures allows the analysis of only a subset of the total naturally occurring microbiota in environmental samples enriched in microorganisms.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1339)

To isolate useful microbial enzymes from uncultured soil microorganisms, a metagenome was isolated from soil samples, and a metagenomic library was constructed by using the pUC19 vector.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1340)

The library was screened for amylase activity, and one clone from among approximately 30, 000 recombinant Escherichia coli clones showed amylase activity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1341)

Sequencing of the clone revealed a novel amylolytic enzyme expressed from a novel gene.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1342)

The putative amylase gene ( amyM ) was overexpressed and purified for characterization.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1343)

Optimal conditions for the enzyme activity of the AmyM protein were 42 degrees C and pH 9. 0 ; Ca2 + stabilized the activity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1344)

The amylase hydrolyzed soluble starch and cyclodextrins to produce high levels of maltose and hydrolyzed pullulan to panose.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1345)

The enzyme showed a high transglycosylation activity, making alpha - ( 1, 4 ) linkages exclusively.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1346)

The hydrolysis and transglycosylation properties of AmyM suggest that it has novel characteristics and can be regarded as an intermediate type of maltogenic amylase, alpha - amylase, and 4 - alpha - glucanotransferase.

Precision: 0.00

Recall: 0.00

F1: 0.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	The hydrolysis and Glycosylationtransglycosylation properties of ProteinAmyM suggest that it has novel characteristics and can be regarded as an intermediate type of maltogenic amylase, alpha - amylase, and 4 - alpha - glucanotransferase.
0.9714	Methylation	The hydrolysis and Glycosylationtransglycosylation properties of AmyM suggest that it has novel characteristics and can be regarded as an intermediate type of maltogenic amylase, alpha - amylase, and Protein4 - alpha - glucanotransferase .

(1347)

4 - Hydroxyphenylpyruvate dioxygenase.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1348)

4 - Hydroxyphenylpyruvate dioxygenase ( HPPD ) is an Fe ( II ) - dependent, non - heme oxygenase that catalyzes the conversion of 4 - hydroxyphenylpyruvate to homogentisate.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1349)

This reaction involves decarboxylation, substituent migration and aromatic oxygenation in a single catalytic cycle.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1350)

HPPD is a member of the alpha - keto acid dependent oxygenases that typically require an alpha - keto acid ( almost exclusively alpha - ketoglutarate ) and molecular oxygen to either oxygenate or oxidize a third molecule.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1351)

As an exception in this class of enzymes HPPD has only two substrates, does not use alpha - ketoglutarate, and incorporates both atoms of dioxygen into the aromatic product, homogentisate.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1352)

The tertiary structure of the enzyme would suggest that its mechanism converged with that of other alpha - keto acid enzymes from an extradiol dioxygenase progenitor.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1353)

The transformation catalyzed by HPPD has both agricultural and therapeutic significance.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1354)

HPPD catalyzes the second step in the pathway for the catabolism of tyrosine, that is common to essentially all aerobic forms of life.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1355)

In plants this pathway has an anabolic branch from homogentisate that forms essential isoprenoid redox cofactors such as plastoquinone and tocopherol.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1356)

Naturally occurring multi - ketone molecules act as allelopathic agents by inhibiting HPPD and preventing the production of homogentisate and hence required redox cofactors.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1357)

This has been the basis for the development of a range of very effective herbicides that are currently used commercially.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1358)

In humans, deficiencies of specific enzymes of the tyrosine catabolism pathway give rise to a number of severe metabolic disorders.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1359)

Interestingly, HPPD inhibitor / herbicide molecules act also as therapeutic agents for a number of debilitating and lethal inborn defects in tyrosine catabolism by preventing the accumulation of toxic metabolites.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1360)

Distinct dynamics and distribution of histone methyl - lysine derivatives in mouse development.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1361)

Histone methylation acts as an epigenetic regulator of chromatin activity through the modification of arginine and lysine residues on histones H3 and H4.

Precision: 66.67

Recall: 100.00

F1: 80.00

Score	Relation	Text (Head - Tail)
0.9995	Methylation	Histone Methylationmethylation acts as an epigenetic regulator of chromatin activity through the modification of Entityarginine and lysine residues on histones H3 and H4.
0.9981	Methylation	Histone Methylationmethylation acts as an epigenetic regulator of chromatin activity through the modification of arginine and Entitylysine residues on histones H3 and H4.
0.9968	Methylation	Histone Methylationmethylation acts as an epigenetic regulator of chromatin activity through the modification of arginine and Entitylysine residues on histones H3 and H4.

(1362)

In the case of lysine, this includes the formation of mono -, di -, or trimethyl groups, each of which is presumed to represent a distinct functional state at the cellular level.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1363)

To examine the potential developmental roles of these modifications, we determined the global patterns of lysine methylation involving K9 on histone H3 and K20 on histone H4 in midgestation mouse embryos.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
1.0000	Methylation	To examine the potential developmental roles of these modifications, we determined the global patterns of lysine Methylationmethylation involving EntityK9 on histone H3 and K20 on histone H4 in midgestation mouse embryos.
0.9971	Methylation	To examine the potential developmental roles of these modifications, we determined the global patterns of lysine Methylationmethylation involving K9 on histone H3 and EntityK20 on histone H4 in midgestation mouse embryos.

(1364)

For each lysine target site, we observed distinct subnuclear distributions of the mono - and trimethyl versions in 10T1 / 2 cells that were conserved within primary cultures and within the 3D - tissue architecture of the embryo.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1365)

Interestingly, three of these modifications, histone H3 trimethyl K9, histone H4 monomethyl K20, and histone H4 trimethyl K20 exhibited marked differences in their distribution within the neuroepithelium.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9970	Methylation	Interestingly, three of these Methylationmodifications , histone H3 trimethyl EntityK9 , histone H4 monomethyl K20, and histone H4 trimethyl K20 exhibited marked differences in their distribution within the neuroepithelium.
0.9966	Methylation	Interestingly, three of these Methylationmodifications , histone H3 trimethyl K9, histone H4 monomethyl EntityK20 , and histone H4 trimethyl K20 exhibited marked differences in their distribution within the neuroepithelium.
0.9941	Methylation	Interestingly, three of these Methylationmodifications , histone H3 trimethyl K9, histone H4 monomethyl K20, and histone H4 trimethyl EntityK20 exhibited marked differences in their distribution within the neuroepithelium.

(1366)

Specifically, both histone H3 trimethyl K9 and H4 monomethyl K20 were elevated in proliferating cells of the neural tube, which in the case of the K9 modification was limited to mitotic cells on the luminal surface.

Precision: 33.33

Recall: 100.00

F1: 50.00

Score	Relation	Text (Head - Tail)
0.9991	Methylation	Specifically, both histone H3 trimethyl K9 and H4 monomethyl K20 were elevated in proliferating cells of the neural tube, which in the case of the EntityK9 Methylationmodification was limited to mitotic cells on the luminal surface.
0.4343	Methylation	Specifically, both histone H3 trimethyl K9 and H4 monomethyl EntityK20 were elevated in proliferating cells of the neural tube, which in the case of the K9 Methylationmodification was limited to mitotic cells on the luminal surface.
0.4136	Methylation	Specifically, both histone H3 trimethyl EntityK9 and H4 monomethyl K20 were elevated in proliferating cells of the neural tube, which in the case of the K9 Methylationmodification was limited to mitotic cells on the luminal surface.

(1367)

In contrast, histone H4 trimethyl K20 was progressively lost from these medial regions and became enriched in differentiating neurons in the ventrolateral neural tube.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1368)

The inverse relationship of histone H4 K20 methyl derivatives is even more striking during skeletal and cardiac myogenesis where the accumulation of the trimethyl modification in pericentromeric heterochromatin suggests a role in gene silencing in postmitotic muscle cells.

Precision: 10.00

Recall: 100.00

F1: 18.18

Score	Relation	Text (Head - Tail)
0.9962	Methylation	The inverse relationship of histone H4 EntityK20 methyl derivatives is even more striking during skeletal and cardiac myogenesis where the accumulation of the Methylationtrimethyl modification in pericentromeric heterochromatin suggests a role in gene silencing in postmitotic muscle cells.
0.9997	Methylation	The inverse relationship of histone H4 EntityK20 Methylationmethyl derivatives is even more striking during skeletal and cardiac myogenesis where the accumulation of the trimethyl modification in pericentromeric heterochromatin suggests a role in gene silencing in postmitotic muscle cells.
0.9964	Methylation	The inverse relationship of histone H4 EntityK20 methyl derivatives is even more striking during skeletal and cardiac myogenesis where the accumulation of the trimethyl Methylationmodification in pericentromeric heterochromatin suggests a role in gene silencing in postmitotic muscle cells.
0.9932	Methylation	The inverse relationship of histone H4 EntityK20 methyl derivatives is even more striking during skeletal and cardiac myogenesis where the accumulation of the Methylationtrimethyl modification in pericentromeric heterochromatin suggests a role in gene silencing in postmitotic muscle cells.
0.6355	Methylation	The inverse relationship of histone H4 K20 methyl derivatives is even more striking during skeletal and cardiac myogenesis where the accumulation of the Methylationtrimethyl modification Methylationmodification in pericentromeric heterochromatin suggests a role in gene silencing in postmitotic muscle cells.
0.5638	Methylation	The inverse relationship of histone H4 K20 methyl derivatives is even more striking during skeletal and cardiac myogenesis where the accumulation of the Methylationtrimethyl modification Methylationmodification in pericentromeric heterochromatin suggests a role in gene silencing in postmitotic muscle cells.
0.5345	Methylation	The inverse relationship of histone H4 K20 methyl derivatives is even more striking during skeletal and cardiac myogenesis where the accumulation of the Methylationtrimethyl Methylationtrimethyl modification in pericentromeric heterochromatin suggests a role in gene silencing in postmitotic muscle cells.
0.5077	Methylation	The inverse relationship of histone H4 K20 methyl derivatives is even more striking during skeletal and cardiac myogenesis where the accumulation of the Methylationtrimethyl Methylationmodification in pericentromeric heterochromatin suggests a role in gene silencing in postmitotic muscle cells.
0.4898	Methylation	The inverse relationship of histone H4 K20 methyl derivatives is even more striking during skeletal and cardiac myogenesis where the accumulation of the Methylationtrimethyl Methylationmodification in pericentromeric heterochromatin suggests a role in gene silencing in postmitotic muscle cells.
0.4421	Methylation	The inverse relationship of histone H4 K20 methyl derivatives is even more striking during skeletal and cardiac myogenesis where the accumulation of the Methylationtrimethyl modification Methylationtrimethyl modification in pericentromeric heterochromatin suggests a role in gene silencing in postmitotic muscle cells.

(1369)

Importantly, our results establish that histone lysine methylation occurs in a highly dynamic manner that is consistent with their function in an epigenetic program for cell division and differentiation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9982	Methylation	Importantly, our results establish that histone Entitylysine Methylationmethylation occurs in a highly dynamic manner that is consistent with their function in an epigenetic program for cell division and differentiation.

(1370)

Decreased expression of lysyl hydroxylase 2 ( LH2 ) in skin fibroblasts from three Ehlers - Danlos patients does not result from mutations in either the coding or proximal promoter region of the LH2 gene.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1371)

The Ehlers - Danlos syndromes ( EDS ) are a heterogeneous group of inherited connective tissue disorders characterized by tissue fragility, hyperelasticity of the skin and joint hypermobility.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1372)

This phenotype, accompanied by kyphoscoliosis and / or ocular fragility, is present in patients with the autosomal recessive type VI form of EDS.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1373)

These patients have significantly decreased levels of lysyl hydroxylase ( LH ) activity, due to mutations in the LH1 gene.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1374)

LH hydroxylates specific lysine residues in the collagen molecule that are precursors for the formation of cross - links which provide collagen with its tensile strength.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1375)

No disorder has been directly linked to decreased expression of LH2 and LH3, two other isoforms of LH.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1376)

This study describes 3 patients with mixed phenotypes of EDS, who have significantly decreased mRNAs for LH2, but normal levels of LH1 and LH3 mRNAs, in their skin fibroblasts.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1377)

In contrast to the effect of LH1 deficiency in EDS VI patients, the decreased expression of LH2 does not affect LH activity, bifunctional collagen cross - links ( measured after reduction as dihydroxylysinonorleucine ( DHLNL ) and hydroxylysinonorleucine ( HLNL ) ), or helical lysine hydroxylation in these cell lines.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1378)

Sequence analysis of full length LH2 cDNAs and 1kb of the promoter region of LH2 does not show mutations that could explain the decreased expression of LH2.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1379)

These results suggest that the deficiency of LH2 in these fibroblasts may be caused by changes in other factors required for the expression of LH2.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1380)

Expression and purification of functionally active hyaluronan - binding domains from human cartilage link protein, aggrecan and versican : formation of ternary complexes with defined hyaluronan oligosaccharides.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1381)

The chondroitin sulfate proteoglycan aggrecan forms link protein - stabilized complexes with hyaluronan ( HA ), via its N - terminal G1 - domain, that provide cartilage with its load bearing properties.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1382)

Similar aggregates ( potentially containing new members of the link protein family ), in which other chondroitin sulfate proteoglycans ( i. e. versican, brevican, and neurocan ) substitute for aggrecan, may contribute to the structural integrity of many other tissues including skin and brain.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1383)

In this study, cartilage link protein ( cLP ) and the G1 - domains of aggrecan ( AG1 ) and versican ( VG1 ) were expressed in Drosophila S2 cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1384)

The recombinant human proteins were found to have properties similar to those described for the native molecules ( e. g. cLP was able to form oligomers, and HA decasaccharides were the minimum size that could compete effectively for their binding to polymeric HA ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1385)

Gel filtration and protein cross - linking / matrix - assisted laser desorption ionization time - of - flight peptide fingerprinting showed that cLP and AG1 interact in the absence or presence of HA.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1386)

Conversely, cLP and VG1 did not bind directly to each other in solution yet formed ternary complexes with HA24.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1387)

N - linked glycosylation of AG1 and VG1 was demonstrated to be unnecessary for either HA binding or the formation of ternary complexes.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1388)

Surprisingly, the length of HA required to accommodate two G1 - domains was found to be significantly larger for aggrecan than versican, which may reflect differences in the conformation of HA stabilized on binding these proteins.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1389)

Protective mechanism of epigallocatechin - 3 - gallate against Helicobacter pylori - induced gastric epithelial cytotoxicity via the blockage of TLR - 4 signaling.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1390)

BACKGROUND : Helicobacter pylori infection leads to gastric mucosal damage by several mechanisms including the direct effect of virulence factors produced by H. pylori, propagation of inflammation, oxidative stress, DNA damage, and induction of apoptosis. ( - ) - Epigallocatechin - 3 - gallate ( EGCG ), one of the green tea catechins, is known to suppress H. pylori - induced gastritis through its antioxidative and antibacterial actions.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1391)

In this study, we evaluated the protective mechanism of EGCG against H. pylori - induced cytotoxicity in gastric epithelial cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1392)

MATERIALS AND METHODS : MTT assays and dye exclusion assays were performed to analyze the effect of EGCG on the viability of gastric epithelial cells.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1393)

The degree of DNA damage was evaluated by Comet assay and apoptotic DNA fragmentation assay.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1394)

To investigate the effect of EGCG on H. pylori - induced toll - like receptor 4 ( TLR - 4 ) signaling, reverse transcription - polymerase chain reaction and Western blot analysis corresponding to glycosylated TLR - 4 were carried out.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9986	Methylation	To investigate the effect of EGCG on H. pylori - induced toll - like receptor 4 ( TLR - 4 ) signaling, reverse transcription - polymerase chain reaction and Western blot analysis corresponding to Glycosylationglycosylated ProteinTLR - 4 were carried out.

(1395)

Lipoxygenase metabolites were measured with reverse - phase, high - performance liquid chromatography.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1396)

RESULTS : EGCG pretreatment effectively rescued gastric mucosal cells from the H. pylori - induced apoptotic cell death and DNA damage, and administration of this catechin enhanced gastric epithelial cell proliferation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1397)

Helicobacter pylori infection stimulated the glycosylation of TLR - 4, which initiates intracellular signaling in the infected host cell, but the pretreatment with EGCG completely blocked the TLR - 4 glycosylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9999	Methylation	Helicobacter pylori infection stimulated the Glycosylationglycosylation of ProteinTLR - 4 , which initiates intracellular signaling in the infected host cell, but the pretreatment with EGCG completely blocked the TLR - 4 glycosylation.
0.9993	Methylation	Helicobacter pylori infection stimulated the glycosylation of TLR - 4, which initiates intracellular signaling in the infected host cell, but the pretreatment with EGCG completely blocked the ProteinTLR - 4 Glycosylationglycosylation .

(1398)

The blockage of TLR - 4 activation by EGCG resulted in inactivation of extracellular signal response kinase 1 / 2 and of nuclear factor - kappaB, the downstream molecules of TLR - 4 signaling induced by H. pylori.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1399)

This disturbance of H. pylori - induced host cell signaling by EGCG attenuated the synthesis of the proinflammatory mediator, hydroxyeicosatetraenoic acid.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1400)

CONCLUSIONS : EGCG pretreatment showed significant cytoprotective effects against H. pylori - induced gastric cytotoxicity via interference of the TLR - 4 signaling induced by H. pylori.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1401)

Thus, our result implies that continuous intakes of green tea could prevent the deleterious consequences of H. pylori infection.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1402)

Bare - faced curassow lysozyme carrying amino acid substitutions at subsites E and F shows a change in activity against chitooligosaccharide caused by a local conformational change.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1403)

A new form of avian lysozyme, bare - faced curassow lysozyme ( BCL ), was purified and chemically sequenced.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1404)

Of the 26 substitutions relative to chicken lysozyme, three, F34Y, T47S, and R114H, are of substrate - interacting residues in the E and F subsites, which would contribute to the acceptor binding for transglycosylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1405)

T47S is a novel substitution in this lysozyme class.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1406)

While other lysozymes also have substitutions at positions 114 and 34, they also contain numerous others, including ones in the other substrate binding sites, A - D.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1407)

Furthermore, T47S lies on the left side, while F34Y and R114H are located on the right side of the E - F subsites.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1408)

BCL therefore should allow comparison of the independent contributions of these sites to substrate binding and transglycosylation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1409)

The activity toward the N - acetylglucosamine pentamer revealed that the substitutions at the E - F sites reduced the binding free energies at the E - F sites and the rate constant for transglycosylation without the conformation change of other substrate binding sites on the protein.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1410)

MD simulation analysis of BCL suggested that the substituted amino acids changed the local conformation of this lysozyme at the E - F sites.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1411)

Histone H2B ubiquitylation is associated with elongating RNA polymerase II.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9959	Methylation	ProteinHistone H2B Ubiquitinationubiquitylation is associated with elongating RNA polymerase II.

(1412)

Rad6 - mediated ubiquitylation of histone H2B at lysine 123 has been linked to transcriptional activation and the regulation of lysine methylation on histone H3.

Precision: 66.67

Recall: 100.00

F1: 80.00

Score	Relation	Text (Head - Tail)
0.9936	Methylation	Rad6 - mediated Ubiquitinationubiquitylation of histone H2B at Entitylysine 123 has been linked to transcriptional activation and the regulation of lysine methylation on histone H3.
0.9927	Methylation	Rad6 - mediated ubiquitylation of histone H2B at lysine 123 has been linked to transcriptional activation and the regulation of Entitylysine Methylationmethylation on histone H3.
0.9764	Methylation	Rad6 - mediated Ubiquitinationubiquitylation of histone H2B at Entitylysine 123 has been linked to transcriptional activation and the regulation of lysine methylation on histone H3.

(1413)

However, how Rad6 and H2B ubiquitylation contribute to the transcription and histone methylation processes is poorly understood.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9985	Methylation	However, how Rad6 and ProteinH2B Ubiquitinationubiquitylation contribute to the transcription and histone methylation processes is poorly understood.
0.9977	Methylation	However, how Rad6 and H2B ubiquitylation contribute to the transcription and Proteinhistone Methylationmethylation processes is poorly understood.

(1414)

Here, we show that the Paf1 transcription elongation complex and the E3 ligase for Rad6, Bre1, mediate an association of Rad6 with the hyperphosphorylated ( elongating ) form of RNA polymerase II ( Pol II ).

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1415)

This association appears to be necessary for the transcriptional activities of Rad6, as deletion of various Paf1 complex members or Bre1 abolishes H2B ubiquitylation ( ubH2B ) and reduces the recruitment of Rad6 to the promoters and transcribed regions of active genes.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Relation	Text (Head - Tail)
0.9945	Methylation	This association appears to be necessary for the transcriptional activities of Rad6, as deletion of various Paf1 complex members or Bre1 abolishes ProteinH2B Ubiquitinationubiquitylation ( ubH2B ) and reduces the recruitment of Rad6 to the promoters and transcribed regions of active genes.
0.7104	Methylation	This association appears to be necessary for the transcriptional activities of Rad6, as deletion of various Paf1 complex members or Bre1 abolishes ProteinH2B Ubiquitinationubiquitylation ( ubH2B ) and reduces the recruitment of Rad6 to the promoters and transcribed regions of active genes.

(1416)

Using the inducible GAL1 gene as a model, we find that the recruitment of Rad6 upon activation occurs rapidly and transiently across the gene and coincides precisely with the appearance of Pol II.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1417)

Significantly, during GAL1 activation in an rtf1 deletion mutant, Rad6 accumulates at the promoter but is absent from the transcribed region.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1418)

This fact suggests that Rad6 is recruited to promoters independently of the Paf1 complex but then requires this complex for entrance into the coding region of genes in a Pol II - associated manner.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1419)

In support of a role for Rad6 - dependent H2B ubiquitylation in transcription elongation, we find that ubH2B levels are dramatically reduced in strains bearing mutations of the Pol II C - terminal domain ( CTD ) and abolished by inactivation of Kin28, the serine 5 CTD kinase that promotes the transition from initiation to elongation.

Precision: 50.00

Recall: 100.00

F1: 66.67

Score	Relation	Text (Head - Tail)
0.9863	Methylation	In support of a role for Rad6 - dependent ProteinH2B Ubiquitinationubiquitylation in transcription elongation, we find that ubH2B levels are dramatically reduced in strains bearing mutations of the Pol II C - terminal domain ( CTD ) and abolished by inactivation of Kin28, the serine 5 CTD kinase that promotes the transition from initiation to elongation.
0.7766	Methylation	In support of a role for ProteinRad6 - dependent H2B Ubiquitinationubiquitylation in transcription elongation, we find that ubH2B levels are dramatically reduced in strains bearing mutations of the Pol II C - terminal domain ( CTD ) and abolished by inactivation of Kin28, the serine 5 CTD kinase that promotes the transition from initiation to elongation.

(1420)

Furthermore, synthetic genetic array analysis reveals that the Rad6 complex interacts genetically with a number of known or suspected transcription elongation factors.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

(1421)

Finally, we show that Saccharomyces cerevisiae mutants bearing defects in the pathway to H2B ubiquitylation display transcription elongation defects as assayed by 6 - azauracil sensitivity.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9990	Methylation	Finally, we show that Saccharomyces cerevisiae mutants bearing defects in the pathway to ProteinH2B Ubiquitinationubiquitylation display transcription elongation defects as assayed by 6 - azauracil sensitivity.

(1422)

Collectively, our results indicate a role for Rad6 and H2B ubiquitylation during the elongation cycle of transcription and suggest a mechanism by which H3 methylation may be regulated.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)
0.9982	Methylation	Collectively, our results indicate a role for Rad6 and ProteinH2B Ubiquitinationubiquitylation during the elongation cycle of transcription and suggest a mechanism by which H3 methylation may be regulated.
0.9982	Methylation	Collectively, our results indicate a role for Rad6 and H2B ubiquitylation during the elongation cycle of transcription and suggest a mechanism by which ProteinH3 Methylationmethylation may be regulated.

(1423)

Glycosylation - related gene expression in prion diseases : PrPSc accumulation in scrapie infected GT1 cells depends on beta - 1, 4 - linked GalNAc - 4 - SO4 hyposulfation.

Precision: 100.00

Recall: 100.00

F1: 100.00

Score	Relation	Text (Head - Tail)

Relation Extraction Examples (1424)